Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              20     299
NP_001269723                                                           17    3301
NP_055705                                                              17    3301
NP_001269722                                                           17    3301
NP_001035924                                                           17    3301
NP_878913                                                              17    3301
XP_006713549                                                           17    3301
NP_003757                                                              17    4312
NP_057204                                                              17    4312
NP_899196                                                              17    4312
XP_005257817                                                           17    4312
XP_005257816                                                           17    4312
XP_005257815                                                           17    4312
NP_078838                                                              16    5631
NP_060890                                                              16    5631
XP_006710817                                                           16    5631
XP_005271062                                                           16    5631
XP_005271061                                                           16    5631
XP_006710816                                                           16    5631
NP_542781                                                              16    5631
NP_001121361                                                           16    7355
NP_057714                                                              16    7355
NP_001121362                                                           16    7355
NP_001098998                                                           16    7355
NP_945346                                                              16    7355
NP_008855                                                              16    7355
NP_001071634                                                           16    7355
NP_003760                                                              16    7355
NP_060020                                                              16    7355
XP_006722833                                                           16    7355
XP_006722832                                                           16    7355
XP_005259639                                                           16    7355
XP_006722075                                                           16    7355
XP_005254494                                                           16    7355
XP_005254493                                                           16    7355
XP_006720627                                                           16    7355
XP_005266998                                                           16    7355
NP_006266                                                              15    9605
NP_001244289                                                           15    9605
NP_005617                                                              15    9605
NP_981949                                                              15    9605
NP_631895                                                              15    9605
NP_001244290                                                           15    9605
NP_004028                                                              15    9605
XP_005268057                                                           15    9605
XP_005268056                                                           15    9605
XP_005268055                                                           15    9605
XP_005270809                                                           15    9605
NP_008856                                                              15    9605
NP_001034554                                                           15    9605
>NP_004110
          Length = 993

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   DAEEAMYQ 9
           DAEEAMYQ
Sbjct: 949 DAEEAMYQ 956
>NP_001269723
          Length = 895

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   TDAEEAMY 8
           TD EE MY
Sbjct: 311 TDVEEKMY 318
>NP_055705
          Length = 893

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   TDAEEAMY 8
           TD EE MY
Sbjct: 311 TDVEEKMY 318
>NP_001269722
          Length = 711

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   TDAEEAMY 8
           TD EE MY
Sbjct: 127 TDVEEKMY 134
>NP_001035924
          Length = 1133

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   TDAEEAMY 8
           TD EE MY
Sbjct: 551 TDVEEKMY 558
>NP_878913
          Length = 1135

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   TDAEEAMY 8
           TD EE MY
Sbjct: 551 TDVEEKMY 558
>XP_006713549
          Length = 893

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   TDAEEAMY 8
           TD EE MY
Sbjct: 311 TDVEEKMY 318
>NP_003757
          Length = 450

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   DAEEAMYQ 9
           D EEA YQ
Sbjct: 223 DQEEAQYQ 230
>NP_057204
          Length = 624

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  DAEEAMYQ 9
          D EEA YQ
Sbjct: 27 DREEAFYQ 34
>NP_899196
          Length = 624

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  DAEEAMYQ 9
          D EEA YQ
Sbjct: 27 DREEAFYQ 34
>XP_005257817
          Length = 355

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   DAEEAMYQ 9
           D EEA YQ
Sbjct: 223 DQEEAQYQ 230
>XP_005257816
          Length = 382

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   DAEEAMYQ 9
           D EEA YQ
Sbjct: 223 DQEEAQYQ 230
>XP_005257815
          Length = 450

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   DAEEAMYQ 9
           D EEA YQ
Sbjct: 223 DQEEAQYQ 230
>NP_078838
          Length = 1094

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   DAEEAMYQ 9
           D +EA+YQ
Sbjct: 559 DEDEALYQ 566
>NP_060890
          Length = 547

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TDAEEAMY 8
           ++AEEA+Y
Sbjct: 259 SEAEEALY 266
>XP_006710817
          Length = 315

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  TDAEEAMY 8
          ++AEEA+Y
Sbjct: 27 SEAEEALY 34
>XP_005271062
          Length = 316

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  TDAEEAMY 8
          ++AEEA+Y
Sbjct: 28 SEAEEALY 35
>XP_005271061
          Length = 320

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TDAEEAMY 8
           ++AEEA+Y
Sbjct: 259 SEAEEALY 266
>XP_006710816
          Length = 476

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TDAEEAMY 8
           ++AEEA+Y
Sbjct: 188 SEAEEALY 195
>NP_542781
          Length = 261

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  DAEEAMY 8
          DAE+A+Y
Sbjct: 63 DAEDALY 69
>NP_001121361
          Length = 308

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TDAEEAMYQ 9
           T+A+E MY+
Sbjct: 232 TEAKEEMYK 240
>NP_057714
          Length = 308

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TDAEEAMYQ 9
           T+A+E MY+
Sbjct: 232 TEAKEEMYK 240
>NP_001121362
          Length = 281

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TDAEEAMYQ 9
           T+A+E MY+
Sbjct: 205 TEAKEEMYK 213
>NP_001098998
          Length = 867

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DAEEAMYQ 9
           DAEEA+ Q
Sbjct: 183 DAEEALKQ 190
>NP_945346
          Length = 829

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DAEEAMYQ 9
           DAEEA+ Q
Sbjct: 183 DAEEALKQ 190
>NP_008855
          Length = 248

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  DAEEAMY 8
          DAE+A+Y
Sbjct: 66 DAEDAVY 72
>NP_001071634
          Length = 201

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  DAEEAMY 8
          DAE+A+Y
Sbjct: 66 DAEDAVY 72
>NP_003760
          Length = 221

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  DAEEAMY 8
          DAE+A+Y
Sbjct: 64 DAEDAIY 70
>NP_060020
          Length = 545

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   DAEEAMYQ 9
           D+E+A+Y+
Sbjct: 266 DSEQALYE 273
>XP_006722833
          Length = 469

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   DAEEAMYQ 9
           D+E+A+Y+
Sbjct: 190 DSEQALYE 197
>XP_006722832
          Length = 540

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   DAEEAMYQ 9
           D+E+A+Y+
Sbjct: 268 DSEQALYE 275
>XP_005259639
          Length = 547

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   DAEEAMYQ 9
           D+E+A+Y+
Sbjct: 268 DSEQALYE 275
>XP_006722075
          Length = 196

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  DAEEAMY 8
          DAE+A+Y
Sbjct: 66 DAEDAVY 72
>XP_005254494
          Length = 308

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TDAEEAMYQ 9
           T+A+E MY+
Sbjct: 232 TEAKEEMYK 240
>XP_005254493
          Length = 308

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TDAEEAMYQ 9
           T+A+E MY+
Sbjct: 232 TEAKEEMYK 240
>XP_006720627
          Length = 308

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TDAEEAMYQ 9
           T+A+E MY+
Sbjct: 232 TEAKEEMYK 240
>XP_005266998
          Length = 762

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TDAEEA 6
           TDAEEA
Sbjct: 610 TDAEEA 615
>NP_006266
          Length = 344

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  DAEEAMYQ 9
          DA++A+Y+
Sbjct: 47 DADDAVYE 54
>NP_001244289
          Length = 879

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TDAEEAMYQ 9
           +D EE+ YQ
Sbjct: 527 SDLEESKYQ 535
>NP_005617
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  DAEEAMYQ 9
          DA++A+Y+
Sbjct: 47 DADDAVYE 54
>NP_981949
          Length = 760

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TDAEEAMYQ 9
           +D EE+ YQ
Sbjct: 408 SDLEESKYQ 416
>NP_631895
          Length = 798

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TDAEEAMYQ 9
           +D EE+ YQ
Sbjct: 446 SDLEESKYQ 454
>NP_001244290
          Length = 761

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TDAEEAMYQ 9
           +D EE+ YQ
Sbjct: 409 SDLEESKYQ 417
>NP_004028
          Length = 879

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TDAEEAMYQ 9
           +D EE+ YQ
Sbjct: 527 SDLEESKYQ 535
>XP_005268057
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  DAEEAMYQ 9
          DA++A+Y+
Sbjct: 49 DADDAVYE 56
>XP_005268056
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  DAEEAMYQ 9
          DA++A+Y+
Sbjct: 49 DADDAVYE 56
>XP_005268055
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  DAEEAMYQ 9
          DA++A+Y+
Sbjct: 49 DADDAVYE 56
>XP_005270809
          Length = 804

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TDAEEAMYQ 9
           +D EE+ YQ
Sbjct: 452 SDLEESKYQ 460
>NP_008856
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  DAEEAMYQ 9
          DA++A+Y+
Sbjct: 49 DADDAVYE 56
>NP_001034554
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  DAEEAMYQ 9
          DA++A+Y+
Sbjct: 49 DADDAVYE 56
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.110    0.276 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 513,293
Number of extensions: 893
Number of successful extensions: 50
Number of sequences better than 10000.0: 50
Number of HSP's gapped: 50
Number of HSP's successfully gapped: 50
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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