Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001257436                                                           22     134
NP_001257437                                                           22     134
NP_006281                                                              22     134
XP_006715618                                                           22     134
XP_005267176                                                           22     134
NP_003817                                                              18    1935
NP_001451                                                              17    4312
NP_001017979                                                           17    4312
NP_001153073                                                           17    4312
NP_004240                                                              17    4312
XP_005248274                                                           17    4312
XP_005248272                                                           17    4312
XP_005245096                                                           17    4312
NP_787054                                                              16    5631
NP_065761                                                              16    7355
NP_006258                                                              16    7355
XP_005264064                                                           16    7355
XP_005264063                                                           16    7355
XP_005264062                                                           16    7355
XP_005264061                                                           16    7355
XP_005264060                                                           16    7355
XP_005264059                                                           16    7355
NP_005545                                                              15    9605
NP_005546                                                              15    9605
NP_775109                                                              15    9605
NP_000414                                                              15    9605
NP_258259                                                              15    9605
NP_056932                                                              15    9605
NP_004684                                                              15    9605
NP_003301                                                              15    9605
NP_476429                                                              15    9605
NP_002263                                                              15    9605
XP_006719456                                                           15    9605
XP_005268916                                                           15    9605
XP_006719455                                                           15    9605
>NP_001257436
          Length = 790

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TCFKRTAAE 9
           TCFKRT AE
Sbjct: 538 TCFKRTTAE 546
>NP_001257437
          Length = 790

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TCFKRTAAE 9
           TCFKRT AE
Sbjct: 538 TCFKRTTAE 546
>NP_006281
          Length = 790

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TCFKRTAAE 9
           TCFKRT AE
Sbjct: 538 TCFKRTTAE 546
>XP_006715618
          Length = 577

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TCFKRTAAE 9
           TCFKRT AE
Sbjct: 325 TCFKRTTAE 333
>XP_005267176
          Length = 790

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TCFKRTAAE 9
           TCFKRT AE
Sbjct: 538 TCFKRTTAE 546
>NP_003817
          Length = 312

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TCFKRTAAE 9
           TC+K+T A+
Sbjct: 192 TCYKKTIAQ 200
>NP_001451
          Length = 471

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TCFKRT 6
          TCF+RT
Sbjct: 29 TCFERT 34
>NP_001017979
          Length = 221

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   CFKRTAAE 9
           CF++ AAE
Sbjct: 169 CFQKVAAE 176
>NP_001153073
          Length = 204

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   CFKRTAAE 9
           CF++ AAE
Sbjct: 169 CFQKVAAE 176
>NP_004240
          Length = 220

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   CFKRTAAE 9
           CF++ AAE
Sbjct: 169 CFQKVAAE 176
>XP_005248274
          Length = 194

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   CFKRTAAE 9
           CF++ AAE
Sbjct: 169 CFQKVAAE 176
>XP_005248272
          Length = 204

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   CFKRTAAE 9
           CF++ AAE
Sbjct: 169 CFQKVAAE 176
>XP_005245096
          Length = 416

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TCFKRT 6
          TCF+RT
Sbjct: 29 TCFERT 34
>NP_787054
          Length = 163

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  TCFKRTA 7
          TC++RT+
Sbjct: 69 TCYRRTS 75
>NP_065761
          Length = 583

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   CFKRTAA 8
           C KRTAA
Sbjct: 367 CEKRTAA 373
>NP_006258
          Length = 3224

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    CFKRTAAE 9
            CFK+T  E
Sbjct: 3032 CFKKTFEE 3039
>XP_005264064
          Length = 2282

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    CFKRTAAE 9
            CFK+T  E
Sbjct: 2090 CFKKTFEE 2097
>XP_005264063
          Length = 3136

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    CFKRTAAE 9
            CFK+T  E
Sbjct: 2944 CFKKTFEE 2951
>XP_005264062
          Length = 3232

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    CFKRTAAE 9
            CFK+T  E
Sbjct: 3040 CFKKTFEE 3047
>XP_005264061
          Length = 3232

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    CFKRTAAE 9
            CFK+T  E
Sbjct: 3040 CFKKTFEE 3047
>XP_005264060
          Length = 3250

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    CFKRTAAE 9
            CFK+T  E
Sbjct: 3058 CFKKTFEE 3065
>XP_005264059
          Length = 3258

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    CFKRTAAE 9
            CFK+T  E
Sbjct: 3066 CFKKTFEE 3073
>NP_005545
          Length = 564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KRTAAE 9
           KRTAAE
Sbjct: 259 KRTAAE 264
>NP_005546
          Length = 564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KRTAAE 9
           KRTAAE
Sbjct: 259 KRTAAE 264
>NP_775109
          Length = 564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KRTAAE 9
           KRTAAE
Sbjct: 259 KRTAAE 264
>NP_000414
          Length = 639

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KRTAAE 9
           KRTAAE
Sbjct: 274 KRTAAE 279
>NP_258259
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KRTAAE 9
           KRTAAE
Sbjct: 226 KRTAAE 231
>NP_056932
          Length = 638

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KRTAAE 9
           KRTAAE
Sbjct: 279 KRTAAE 284
>NP_004684
          Length = 551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KRTAAE 9
           KRTAAE
Sbjct: 245 KRTAAE 250
>NP_003301
          Length = 387

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1  TCFKRTA 7
          TC+ RT+
Sbjct: 82 TCYNRTS 88
>NP_476429
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KRTAAE 9
           KRTAAE
Sbjct: 296 KRTAAE 301
>NP_002263
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KRTAAE 9
           KRTAAE
Sbjct: 233 KRTAAE 238
>XP_006719456
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KRTAAE 9
           KRTAAE
Sbjct: 248 KRTAAE 253
>XP_005268916
          Length = 606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KRTAAE 9
           KRTAAE
Sbjct: 274 KRTAAE 279
>XP_006719455
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KRTAAE 9
           KRTAAE
Sbjct: 284 KRTAAE 289
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.123    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 431,262
Number of extensions: 286
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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