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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000213                                                              20     390
NP_001087241                                                           20     390
XP_005265799                                                           20     390
XP_005265798                                                           20     390
XP_005265797                                                           20     390
NP_055318                                                              16    5631
XP_006719406                                                           16    5631
NP_009210                                                              16    7355
NP_653223                                                              16    7355
NP_001012496                                                           16    7355
NP_001273205                                                           16    7355
NP_001273203                                                           16    7355
NP_065982                                                              16    7355
NP_001273204                                                           16    7355
XP_006723057                                                           16    7355
NP_612144                                                              16    7355
NP_996816                                                              15    9605
NP_203748                                                              15    9605
NP_055309                                                              15    9605
NP_056432                                                              15    9605
XP_006721103                                                           15    9605
XP_005255314                                                           15    9605
XP_006721102                                                           15    9605
XP_005255313                                                           15    9605
XP_005255312                                                           15    9605
XP_006721101                                                           15    9605
XP_005255311                                                           15    9605
XP_005255310                                                           15    9605
XP_005254357                                                           15    9605
XP_005271772                                                           15    9605

>NP_000213
          Length = 976

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TAYGLIKSN 9
           TAYGLIKS+
Sbjct: 607 TAYGLIKSD 615



>NP_001087241
          Length = 972

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TAYGLIKSN 9
           TAYGLIKS+
Sbjct: 603 TAYGLIKSD 611



>XP_005265799
          Length = 973

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TAYGLIKSN 9
           TAYGLIKS+
Sbjct: 604 TAYGLIKSD 612



>XP_005265798
          Length = 976

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TAYGLIKSN 9
           TAYGLIKS+
Sbjct: 608 TAYGLIKSD 616



>XP_005265797
          Length = 977

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TAYGLIKSN 9
           TAYGLIKS+
Sbjct: 608 TAYGLIKSD 616



>NP_055318
          Length = 2785

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    TAYGLIKS 8
            TA+GL+K+
Sbjct: 2223 TAFGLLKA 2230



>XP_006719406
          Length = 2380

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    TAYGLIKS 8
            TA+GL+K+
Sbjct: 2223 TAFGLLKA 2230



>NP_009210
          Length = 475

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YGLIKS 8
           YGL+KS
Sbjct: 409 YGLVKS 414



>NP_653223
          Length = 773

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  TAYGLIK 7
          TAYGL++
Sbjct: 38 TAYGLLE 44



>NP_001012496
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YGLIKS 8
           YGL+KS
Sbjct: 405 YGLVKS 410



>NP_001273205
          Length = 720

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   TAYGLIKSN 9
           T Y L+K N
Sbjct: 185 TVYSLVKHN 193



>NP_001273203
          Length = 760

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   TAYGLIKSN 9
           T Y L+K N
Sbjct: 225 TVYSLVKHN 233



>NP_065982
          Length = 787

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   TAYGLIKSN 9
           T Y L+K N
Sbjct: 252 TVYSLVKHN 260



>NP_001273204
          Length = 720

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   TAYGLIKSN 9
           T Y L+K N
Sbjct: 185 TVYSLVKHN 193



>XP_006723057
          Length = 311

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YGLIKS 8
           YGL+KS
Sbjct: 245 YGLVKS 250



>NP_612144
          Length = 740

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TAYGLIKSN 9
           T+YG+ K N
Sbjct: 601 TSYGIQKGN 609



>NP_996816
          Length = 5202

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    TAYGLIKSN 9
            T Y  +KSN
Sbjct: 3091 TIYACVKSN 3099



>NP_203748
          Length = 883

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GLIKSN 9
           GL+KSN
Sbjct: 463 GLVKSN 468



>NP_055309
          Length = 1962

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AYGLIKSN 9
           A G++KSN
Sbjct: 810 ATGMVKSN 817



>NP_056432
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TAYGLI 6
           TAYGL+
Sbjct: 377 TAYGLL 382



>XP_006721103
          Length = 1597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AYGLIKSN 9
           A G++KSN
Sbjct: 778 ATGMVKSN 785



>XP_005255314
          Length = 1709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AYGLIKSN 9
           A G++KSN
Sbjct: 557 ATGMVKSN 564



>XP_006721102
          Length = 1820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AYGLIKSN 9
           A G++KSN
Sbjct: 668 ATGMVKSN 675



>XP_005255313
          Length = 1822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AYGLIKSN 9
           A G++KSN
Sbjct: 778 ATGMVKSN 785



>XP_005255312
          Length = 1871

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AYGLIKSN 9
           A G++KSN
Sbjct: 778 ATGMVKSN 785



>XP_006721101
          Length = 1907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AYGLIKSN 9
           A G++KSN
Sbjct: 778 ATGMVKSN 785



>XP_005255311
          Length = 1913

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AYGLIKSN 9
           A G++KSN
Sbjct: 810 ATGMVKSN 817



>XP_005255310
          Length = 1919

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AYGLIKSN 9
           A G++KSN
Sbjct: 778 ATGMVKSN 785



>XP_005254357
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TAYGLI 6
           TAYGL+
Sbjct: 377 TAYGLL 382



>XP_005271772
          Length = 884

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GLIKSN 9
           GL+KSN
Sbjct: 464 GLVKSN 469



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.127    0.342 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 521,099
Number of extensions: 996
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)