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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_036342                                                              17    3301
NP_001093149                                                           17    3301
XP_005251870                                                           17    3301
NP_004781                                                              16    5631
NP_695008                                                              16    5631
NP_695010                                                              16    5631
NP_695009                                                              16    5631
NP_694551                                                              16    7355
NP_005553                                                              15    9605
NP_149024                                                              15    9605
NP_061486                                                              15    9605
NP_055873                                                              15    9605
NP_064577                                                              15    9605
XP_006719854                                                           15    9605
XP_006719853                                                           15    9605
XP_006711379                                                           15    9605
XP_005245207                                                           15    9605

>NP_036342
          Length = 653

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TASLAKELT 9
           TASL +ELT
Sbjct: 266 TASLPRELT 274



>NP_001093149
          Length = 653

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TASLAKELT 9
           TASL +ELT
Sbjct: 266 TASLPRELT 274



>XP_005251870
          Length = 653

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TASLAKELT 9
           TASL +ELT
Sbjct: 266 TASLPRELT 274



>NP_004781
          Length = 563

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ASLAKELT 9
           ASL KELT
Sbjct: 311 ASLQKELT 318



>NP_695008
          Length = 550

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ASLAKELT 9
           ASL KELT
Sbjct: 311 ASLQKELT 318



>NP_695010
          Length = 519

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ASLAKELT 9
           ASL KELT
Sbjct: 311 ASLQKELT 318



>NP_695009
          Length = 506

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ASLAKELT 9
           ASL KELT
Sbjct: 311 ASLQKELT 318



>NP_694551
          Length = 534

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TASLAKEL 8
           TASLAK L
Sbjct: 278 TASLAKAL 285



>NP_005553
          Length = 1193

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TASLAKELT 9
           T SLA++LT
Sbjct: 823 TKSLAQQLT 831



>NP_149024
          Length = 159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  ASLAKEL 8
          ASLAKE+
Sbjct: 91 ASLAKEM 97



>NP_061486
          Length = 1111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TASLAKELT 9
           T SLA++LT
Sbjct: 823 TKSLAQQLT 831



>NP_055873
          Length = 1905

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TASLAKELT 9
            +  LAKELT
Sbjct: 1033 SVQLAKELT 1041



>NP_064577
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TASLAKEL 8
           TASL K+L
Sbjct: 206 TASLVKQL 213



>XP_006719854
          Length = 1550

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TASLAKELT 9
           +  LAKELT
Sbjct: 678 SVQLAKELT 686



>XP_006719853
          Length = 1809

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TASLAKELT 9
           +  LAKELT
Sbjct: 937 SVQLAKELT 945



>XP_006711379
          Length = 1208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TASLAKELT 9
           T SLA++LT
Sbjct: 886 TKSLAQQLT 894



>XP_005245207
          Length = 1256

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TASLAKELT 9
           T SLA++LT
Sbjct: 886 TKSLAQQLT 894



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.295    0.105    0.231 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 328,988
Number of extensions: 445
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)