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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           19     665
NP_000170                                                              19     665
NP_001268422                                                           19     665
NP_001268423                                                           19     665
XP_005264328                                                           19     665
NP_000513                                                              16    5631

>NP_001268421
          Length = 1230

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    TASILMHA 8
            TASILMHA
Sbjct: 1067 TASILMHA 1074



>NP_000170
          Length = 1360

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    TASILMHA 8
            TASILMHA
Sbjct: 1197 TASILMHA 1204



>NP_001268422
          Length = 1058

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TASILMHA 8
           TASILMHA
Sbjct: 895 TASILMHA 902



>NP_001268423
          Length = 1058

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   TASILMHA 8
           TASILMHA
Sbjct: 895 TASILMHA 902



>XP_005264328
          Length = 1261

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    TASILMHA 8
            TASILMHA
Sbjct: 1098 TASILMHA 1105



>NP_000513
          Length = 388

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1 TASILMH 7
         TAS+L+H
Sbjct: 2 TASVLLH 8



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.128    0.336 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 401,325
Number of extensions: 359
Number of successful extensions: 6
Number of sequences better than 10000.0: 6
Number of HSP's gapped: 6
Number of HSP's successfully gapped: 6
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)