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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005350                                                              18    1135
NP_004463                                                              17    4312
NP_056144                                                              17    4312
XP_006722774                                                           17    4312
XP_005259895                                                           17    4312
XP_005259894                                                           17    4312
NP_001156757                                                           16    7355
NP_001156758                                                           16    7355
NP_005234                                                              16    7355
NP_001156759                                                           16    7355
NP_053733                                                              16    7355
XP_005261447                                                           16    7355
XP_006724230                                                           16    7355
XP_006724229                                                           16    7355
XP_005261446                                                           16    7355
NP_056224                                                              15    9605
NP_002527                                                              15    9605

>NP_005350
          Length = 552

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TAQASAAAQ 9
           TAQA+AAAQ
Sbjct: 453 TAQAAAAAQ 461



>NP_004463
          Length = 465

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AQASAAAQ 9
           AQA+AAAQ
Sbjct: 382 AQAAAAAQ 389



>NP_056144
          Length = 613

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  AQASAAAQ 9
          AQA+AAAQ
Sbjct: 3  AQAAAAAQ 10



>XP_006722774
          Length = 531

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  AQASAAAQ 9
          AQA+AAAQ
Sbjct: 42 AQAAAAAQ 49



>XP_005259895
          Length = 624

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  AQASAAAQ 9
          AQA+AAAQ
Sbjct: 42 AQAAAAAQ 49



>XP_005259894
          Length = 651

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  AQASAAAQ 9
          AQA+AAAQ
Sbjct: 42 AQAAAAAQ 49



>NP_001156757
          Length = 655

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TAQASAAAQ 9
           T QAS AAQ
Sbjct: 107 TTQASYAAQ 115



>NP_001156758
          Length = 600

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TAQASAAAQ 9
           T QAS AAQ
Sbjct: 107 TTQASYAAQ 115



>NP_005234
          Length = 656

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TAQASAAAQ 9
           T QAS AAQ
Sbjct: 107 TTQASYAAQ 115



>NP_001156759
          Length = 354

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TAQASAAAQ 9
           T QAS AAQ
Sbjct: 107 TTQASYAAQ 115



>NP_053733
          Length = 661

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TAQASAAAQ 9
           T QAS AAQ
Sbjct: 113 TTQASYAAQ 121



>XP_005261447
          Length = 613

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TAQASAAAQ 9
           T QAS AAQ
Sbjct: 107 TTQASYAAQ 115



>XP_006724230
          Length = 639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TAQASAAAQ 9
           T QAS AAQ
Sbjct: 107 TTQASYAAQ 115



>XP_006724229
          Length = 652

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TAQASAAAQ 9
           T QAS AAQ
Sbjct: 107 TTQASYAAQ 115



>XP_005261446
          Length = 669

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TAQASAAAQ 9
           T QAS AAQ
Sbjct: 107 TTQASYAAQ 115



>NP_056224
          Length = 3123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    TAQASAAA 8
            T+QASA+A
Sbjct: 1490 TSQASASA 1497



>NP_002527
          Length = 688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  AQASAAAQ 9
          AQA+AAA+
Sbjct: 22 AQAAAAAE 29



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.285   0.0815    0.169 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 162,378
Number of extensions: 255
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)