Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000305                                                              18    1135
XP_006717990                                                           18    1135
XP_006717989                                                           18    1135
NP_001245210                                                           18    1482
NP_000242                                                              18    1482
NP_733841                                                              18    1482
NP_004671                                                              18    1482
NP_001003895                                                           18    1482
XP_005264389                                                           18    1482
NP_001003894                                                           18    1482
NP_004816                                                              17    3301
XP_005259889                                                           17    3301
NP_001001722                                                           17    3301
NP_001035706                                                           17    4312
XP_005255000                                                           17    4312
XP_005254998                                                           17    4312
NP_001229792                                                           15    9605
NP_060463                                                              15    9605
NP_056040                                                              15    9605
NP_001121087                                                           15    9605
NP_036443                                                              15    9605
XP_006719905                                                           15    9605
XP_006719904                                                           15    9605
XP_005266496                                                           15    9605
XP_005266495                                                           15    9605
XP_006717498                                                           15    9605
XP_006717497                                                           15    9605
XP_005251811                                                           15    9605
XP_005251810                                                           15    9605
XP_005251809                                                           15    9605
XP_005251808                                                           15    9605
XP_005251807                                                           15    9605
XP_005251805                                                           15    9605
XP_005251803                                                           15    9605
XP_006717050                                                           15    9605
XP_005251802                                                           15    9605
XP_006717049                                                           15    9605
XP_006717048                                                           15    9605
XP_006717047                                                           15    9605
XP_006717046                                                           15    9605
XP_006717045                                                           15    9605
>NP_000305
          Length = 403

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  TAIIKEIVN 9
          TAIIKEIV+
Sbjct: 2  TAIIKEIVS 10
>XP_006717990
          Length = 175

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  TAIIKEIVN 9
          TAIIKEIV+
Sbjct: 2  TAIIKEIVS 10
>XP_006717989
          Length = 388

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  TAIIKEIVN 9
          TAIIKEIV+
Sbjct: 2  TAIIKEIVS 10
>NP_001245210
          Length = 868

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AIIKEIVN 9
           AI+KEIVN
Sbjct: 510 AIVKEIVN 517
>NP_000242
          Length = 934

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AIIKEIVN 9
           AI+KEIVN
Sbjct: 576 AIVKEIVN 583
>NP_733841
          Length = 540

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TAIIKEIVN 9
           T +IKEIVN
Sbjct: 311 TEVIKEIVN 319
>NP_004671
          Length = 554

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TAIIKEIVN 9
           T +IKEIVN
Sbjct: 311 TEVIKEIVN 319
>NP_001003895
          Length = 554

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TAIIKEIVN 9
           T +IKEIVN
Sbjct: 311 TEVIKEIVN 319
>XP_005264389
          Length = 921

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AIIKEIVN 9
           AI+KEIVN
Sbjct: 576 AIVKEIVN 583
>NP_001003894
          Length = 540

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TAIIKEIVN 9
           T +IKEIVN
Sbjct: 311 TEVIKEIVN 319
>NP_004816
          Length = 541

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TAIIKEIVN 9
           T +IKE+VN
Sbjct: 312 TEVIKEMVN 320
>XP_005259889
          Length = 1130

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TAIIKEIVN 9
           TAI KE++N
Sbjct: 417 TAICKEVLN 425
>NP_001001722
          Length = 541

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TAIIKEIVN 9
           T +IKE+VN
Sbjct: 312 TEVIKEMVN 320
>NP_001035706
          Length = 757

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   TAIIKEI 7
           TAIIKEI
Sbjct: 195 TAIIKEI 201
>XP_005255000
          Length = 721

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   TAIIKEI 7
           TAIIKEI
Sbjct: 195 TAIIKEI 201
>XP_005254998
          Length = 757

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   TAIIKEI 7
           TAIIKEI
Sbjct: 195 TAIIKEI 201
>NP_001229792
          Length = 643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AIIKEIVN 9
           A++KEI++
Sbjct: 109 AVVKEIIH 116
>NP_060463
          Length = 643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AIIKEIVN 9
           A++KEI++
Sbjct: 109 AVVKEIIH 116
>NP_056040
          Length = 3088

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   IIKEIVN 9
           ++KE++N
Sbjct: 348 VVKEVIN 354
>NP_001121087
          Length = 643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AIIKEIVN 9
           A++KEI++
Sbjct: 109 AVVKEIIH 116
>NP_036443
          Length = 393

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AIIKEIVN 9
           AI+KE+++
Sbjct: 303 AIVKEVID 310
>XP_006719905
          Length = 643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AIIKEIVN 9
           A++KEI++
Sbjct: 109 AVVKEIIH 116
>XP_006719904
          Length = 643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AIIKEIVN 9
           A++KEI++
Sbjct: 109 AVVKEIIH 116
>XP_005266496
          Length = 643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AIIKEIVN 9
           A++KEI++
Sbjct: 109 AVVKEIIH 116
>XP_005266495
          Length = 678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AIIKEIVN 9
           A++KEI++
Sbjct: 144 AVVKEIIH 151
>XP_006717498
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AIIKEIVN 9
           AI+KE+++
Sbjct: 197 AIVKEVID 204
>XP_006717497
          Length = 394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AIIKEIVN 9
           AI+KE+++
Sbjct: 303 AIVKEVID 310
>XP_005251811
          Length = 3060

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   IIKEIVN 9
           ++KE++N
Sbjct: 348 VVKEVIN 354
>XP_005251810
          Length = 3062

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   IIKEIVN 9
           ++KE++N
Sbjct: 348 VVKEVIN 354
>XP_005251809
          Length = 3063

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   IIKEIVN 9
           ++KE++N
Sbjct: 348 VVKEVIN 354
>XP_005251808
          Length = 3075

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   IIKEIVN 9
           ++KE++N
Sbjct: 348 VVKEVIN 354
>XP_005251807
          Length = 3088

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   IIKEIVN 9
           ++KE++N
Sbjct: 348 VVKEVIN 354
>XP_005251805
          Length = 3090

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   IIKEIVN 9
           ++KE++N
Sbjct: 348 VVKEVIN 354
>XP_005251803
          Length = 3091

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   IIKEIVN 9
           ++KE++N
Sbjct: 348 VVKEVIN 354
>XP_006717050
          Length = 3092

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   IIKEIVN 9
           ++KE++N
Sbjct: 348 VVKEVIN 354
>XP_005251802
          Length = 3092

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   IIKEIVN 9
           ++KE++N
Sbjct: 348 VVKEVIN 354
>XP_006717049
          Length = 3100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   IIKEIVN 9
           ++KE++N
Sbjct: 348 VVKEVIN 354
>XP_006717048
          Length = 3101

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   IIKEIVN 9
           ++KE++N
Sbjct: 348 VVKEVIN 354
>XP_006717047
          Length = 3101

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   IIKEIVN 9
           ++KE++N
Sbjct: 348 VVKEVIN 354
>XP_006717046
          Length = 3103

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   IIKEIVN 9
           ++KE++N
Sbjct: 348 VVKEVIN 354
>XP_006717045
          Length = 3104

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   IIKEIVN 9
           ++KE++N
Sbjct: 348 VVKEVIN 354
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.135    0.319 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 382,354
Number of extensions: 498
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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