Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_065681                                                              22     103
NP_066124                                                              22     103
XP_006717999                                                           22     103
NP_003233                                                              17    2528
NP_001020018                                                           17    2528
NP_996531                                                              17    2528
XP_006719978                                                           17    2528
XP_005254087                                                           17    2528
XP_006713379                                                           17    2528
NP_001607                                                              17    3301
NP_001265509                                                           17    3301
NP_001265508                                                           17    3301
NP_055300                                                              17    3301
XP_005266067                                                           17    3301
XP_005262989                                                           17    3301
XP_005263900                                                           17    3301
NP_689490                                                              17    3301
NP_689943                                                              17    4312
NP_067055                                                              17    4312
NP_065102                                                              17    4312
XP_005266918                                                           17    4312
XP_005246870                                                           17    4312
XP_005246869                                                           17    4312
NP_001123917                                                           17    4312
NP_001274432                                                           17    4312
NP_006742                                                              17    4312
NP_689647                                                              16    5631
NP_055177                                                              16    7355
NP_001124335                                                           16    7355
NP_932164                                                              16    7355
NP_006200                                                              16    7355
NP_001035181                                                           16    7355
NP_001230280                                                           16    7355
NP_057202                                                              16    7355
NP_005654                                                              16    7355
NP_066015                                                              16    7355
XP_005258381                                                           16    7355
XP_006722580                                                           16    7355
XP_006722579                                                           16    7355
XP_005258380                                                           16    7355
XP_006716650                                                           16    7355
XP_006716649                                                           16    7355
XP_006716648                                                           16    7355
XP_006716647                                                           16    7355
XP_005250069                                                           16    7355
XP_005250068                                                           16    7355
XP_005250067                                                           16    7355
XP_006713980                                                           16    7355
XP_006713979                                                           16    7355
NP_055766                                                              15    9605
NP_821174                                                              15    9605
NP_060340                                                              15    9605
XP_003960868                                                           15    9605
XP_003846356                                                           15    9605
XP_005270777                                                           15    9605
XP_006710615                                                           15    9605
XP_005270776                                                           15    9605
XP_003846691                                                           15    9605
NP_694991                                                              15    9605
>NP_065681
          Length = 1072

 Score = 21.9 bits (45), Expect =   103
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TAFHMKGRA 9
           TAFH+KGRA
Sbjct: 742 TAFHLKGRA 750
>NP_066124
          Length = 1114

 Score = 21.9 bits (45), Expect =   103
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TAFHMKGRA 9
           TAFH+KGRA
Sbjct: 742 TAFHLKGRA 750
>XP_006717999
          Length = 1207

 Score = 21.9 bits (45), Expect =   103
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TAFHMKGRA 9
           TAFH+KGRA
Sbjct: 835 TAFHLKGRA 843
>NP_003233
          Length = 567

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   TAFHMKG 7
           TAFH KG
Sbjct: 325 TAFHAKG 331
>NP_001020018
          Length = 592

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   TAFHMKG 7
           TAFH KG
Sbjct: 350 TAFHAKG 356
>NP_996531
          Length = 340

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AFHMKGR 8
           +FH+KGR
Sbjct: 152 SFHLKGR 158
>XP_006719978
          Length = 340

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AFHMKGR 8
           +FH+KGR
Sbjct: 152 SFHLKGR 158
>XP_005254087
          Length = 340

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AFHMKGR 8
           +FH+KGR
Sbjct: 152 SFHLKGR 158
>XP_006713379
          Length = 628

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   TAFHMKG 7
           TAFH KG
Sbjct: 386 TAFHAKG 392
>NP_001607
          Length = 513

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   TAFHMKG 7
           TAFH KG
Sbjct: 267 TAFHEKG 273
>NP_001265509
          Length = 405

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   TAFHMKG 7
           TAFH KG
Sbjct: 159 TAFHEKG 165
>NP_001265508
          Length = 513

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   TAFHMKG 7
           TAFH KG
Sbjct: 267 TAFHEKG 273
>NP_055300
          Length = 199

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  TAFHMKGRA 9
          T F+M+GRA
Sbjct: 7  TYFNMRGRA 15
>XP_005266067
          Length = 190

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   TAFHMKGR 8
           T FHMK R
Sbjct: 129 TVFHMKRR 136
>XP_005262989
          Length = 168

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  TAFHMKGRA 9
          T F+M+GRA
Sbjct: 7  TYFNMRGRA 15
>XP_005263900
          Length = 361

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   TAFHMKG 7
           TAFH KG
Sbjct: 267 TAFHEKG 273
>NP_689490
          Length = 234

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   TAFHMKGR 8
           T FHMK R
Sbjct: 173 TVFHMKRR 180
>NP_689943
          Length = 1257

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TAFHMKGRA 9
            +AFHM G A
Sbjct: 1144 SAFHMSGFA 1152
>NP_067055
          Length = 536

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   AFHMKG 7
           AFH+KG
Sbjct: 104 AFHLKG 109
>NP_065102
          Length = 498

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TAFHMKGR 8
           TAFHM+ R
Sbjct: 213 TAFHMQVR 220
>XP_005266918
          Length = 1298

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TAFHMKGRA 9
            +AFHM G A
Sbjct: 1185 SAFHMSGFA 1193
>XP_005246870
          Length = 1252

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FHMKGRA 9
           FH KGR+
Sbjct: 147 FHQKGRS 153
>XP_005246869
          Length = 1255

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FHMKGRA 9
           FH KGR+
Sbjct: 147 FHQKGRS 153
>NP_001123917
          Length = 1259

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FHMKGRA 9
           FH KGR+
Sbjct: 147 FHQKGRS 153
>NP_001274432
          Length = 1237

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FHMKGRA 9
           FH KGR+
Sbjct: 147 FHQKGRS 153
>NP_006742
          Length = 1256

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FHMKGRA 9
           FH KGR+
Sbjct: 147 FHQKGRS 153
>NP_689647
          Length = 894

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AFHMKGR 8
           +FH KGR
Sbjct: 378 SFHSKGR 384
>NP_055177
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TAFHMKGR 8
           T F +KGR
Sbjct: 198 TGFRLKGR 205
>NP_001124335
          Length = 888

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   FHMKGR 8
           FH++GR
Sbjct: 242 FHLRGR 247
>NP_932164
          Length = 338

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TAFHMKGR 8
           T F +KGR
Sbjct: 198 TGFRLKGR 205
>NP_006200
          Length = 915

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   FHMKGR 8
           FH++GR
Sbjct: 242 FHLRGR 247
>NP_001035181
          Length = 863

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   FHMKGR 8
           FH++GR
Sbjct: 242 FHLRGR 247
>NP_001230280
          Length = 639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FHMKGRA 9
           +H KGRA
Sbjct: 263 YHEKGRA 269
>NP_057202
          Length = 625

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FHMKGRA 9
           +H KGRA
Sbjct: 263 YHEKGRA 269
>NP_005654
          Length = 539

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   FHMKGR 8
           FH KGR
Sbjct: 208 FHAKGR 213
>NP_066015
          Length = 2579

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TAFHMKGRA 9
           +A H +GRA
Sbjct: 410 SAIHQQGRA 418
>XP_005258381
          Length = 2224

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  TAFHMKGRA 9
          +A H +GRA
Sbjct: 55 SAIHQQGRA 63
>XP_006722580
          Length = 2532

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TAFHMKGRA 9
           +A H +GRA
Sbjct: 410 SAIHQQGRA 418
>XP_006722579
          Length = 2544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TAFHMKGRA 9
           +A H +GRA
Sbjct: 410 SAIHQQGRA 418
>XP_005258380
          Length = 2578

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TAFHMKGRA 9
           +A H +GRA
Sbjct: 410 SAIHQQGRA 418
>XP_006716650
          Length = 911

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   FHMKGR 8
           FH++GR
Sbjct: 242 FHLRGR 247
>XP_006716649
          Length = 936

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   FHMKGR 8
           FH++GR
Sbjct: 238 FHLRGR 243
>XP_006716648
          Length = 936

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   FHMKGR 8
           FH++GR
Sbjct: 242 FHLRGR 247
>XP_006716647
          Length = 940

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   FHMKGR 8
           FH++GR
Sbjct: 242 FHLRGR 247
>XP_005250069
          Length = 590

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FHMKGRA 9
           +H KGRA
Sbjct: 263 YHEKGRA 269
>XP_005250068
          Length = 615

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FHMKGRA 9
           +H KGRA
Sbjct: 239 YHEKGRA 245
>XP_005250067
          Length = 638

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FHMKGRA 9
           +H KGRA
Sbjct: 262 YHEKGRA 268
>XP_006713980
          Length = 458

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   FHMKGR 8
           FH KGR
Sbjct: 127 FHAKGR 132
>XP_006713979
          Length = 564

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   FHMKGR 8
           FH KGR
Sbjct: 233 FHAKGR 238
>NP_055766
          Length = 407

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   FHMKGR 8
           FH KGR
Sbjct: 339 FHPKGR 344
>NP_821174
          Length = 1023

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   FHMKGR 8
           FH+KG+
Sbjct: 143 FHLKGQ 148
>NP_060340
          Length = 1024

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   FHMKGR 8
           FH+KG+
Sbjct: 143 FHLKGQ 148
>XP_003960868
          Length = 208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  HMKGRA 9
          HMKG A
Sbjct: 6  HMKGEA 11
>XP_003846356
          Length = 208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  HMKGRA 9
          HMKG A
Sbjct: 6  HMKGEA 11
>XP_005270777
          Length = 453

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   AFHMKG 7
           +FH+KG
Sbjct: 224 SFHLKG 229
>XP_006710615
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   AFHMKG 7
           +FH+KG
Sbjct: 196 SFHLKG 201
>XP_005270776
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   AFHMKG 7
           +FH+KG
Sbjct: 196 SFHLKG 201
>XP_003846691
          Length = 208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  HMKGRA 9
          HMKG A
Sbjct: 6  HMKGEA 11
>NP_694991
          Length = 566

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   AFHMKG 7
           +FH+KG
Sbjct: 224 SFHLKG 229
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.131    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 591,880
Number of extensions: 702
Number of successful extensions: 59
Number of sequences better than 10000.0: 59
Number of HSP's gapped: 59
Number of HSP's successfully gapped: 59
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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