Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001167571                                                           17    2528
NP_127503                                                              17    2528
NP_127501                                                              17    2528
NP_065151                                                              17    2528
XP_006713333                                                           17    2528
XP_005265389                                                           17    2528
XP_005265388                                                           17    2528
XP_006713332                                                           17    2528
XP_006713331                                                           17    2528
XP_006713330                                                           17    2528
XP_006713329                                                           17    2528
XP_005265387                                                           17    2528
NP_060501                                                              17    3301
NP_000284                                                              17    4312
NP_001027005                                                           17    4312
NP_079335                                                              17    4312
XP_005256041                                                           17    4312
XP_005256040                                                           17    4312
XP_006715631                                                           17    4312
XP_006710061                                                           17    4312
XP_006710060                                                           17    4312
NP_003238                                                              16    5631
NP_689594                                                              16    5631
XP_006712319                                                           16    5631
NP_001186073                                                           16    5631
NP_003822                                                              16    5631
NP_002833                                                              16    7355
NP_001154912                                                           16    7355
NP_055968                                                              16    7355
NP_001277188                                                           16    7355
NP_009016                                                              16    7355
NP_065831                                                              16    7355
XP_006723375                                                           16    7355
XP_006715490                                                           16    7355
XP_005248760                                                           16    7355
XP_005248759                                                           16    7355
XP_006715489                                                           16    7355
XP_005248758                                                           16    7355
XP_006711904                                                           16    7355
NP_001026                                                              15    9605
NP_061027                                                              15    9605
NP_115518                                                              15    9605
NP_057058                                                              15    9605
XP_006722394                                                           15    9605
XP_005258167                                                           15    9605
XP_006722393                                                           15    9605
XP_006722392                                                           15    9605
XP_006722391                                                           15    9605
XP_006722390                                                           15    9605
XP_005258166                                                           15    9605
XP_006722389                                                           15    9605
XP_005258165                                                           15    9605
XP_005258164                                                           15    9605
XP_005266473                                                           15    9605
XP_006711873                                                           15    9605
XP_006711872                                                           15    9605
XP_006711871                                                           15    9605
XP_006711870                                                           15    9605
XP_006711869                                                           15    9605
XP_006711868                                                           15    9605
XP_006711867                                                           15    9605
XP_006711866                                                           15    9605
XP_006711865                                                           15    9605
>NP_001167571
          Length = 403

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TACLSTSK 8
           TACL T+K
Sbjct: 306 TACLETAK 313
>NP_127503
          Length = 403

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TACLSTSK 8
           TACL T+K
Sbjct: 306 TACLETAK 313
>NP_127501
          Length = 403

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TACLSTSK 8
           TACL T+K
Sbjct: 306 TACLETAK 313
>NP_065151
          Length = 360

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TACLSTSK 8
           TACL T+K
Sbjct: 263 TACLETAK 270
>XP_006713333
          Length = 360

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TACLSTSK 8
           TACL T+K
Sbjct: 263 TACLETAK 270
>XP_005265389
          Length = 383

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TACLSTSK 8
           TACL T+K
Sbjct: 286 TACLETAK 293
>XP_005265388
          Length = 403

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TACLSTSK 8
           TACL T+K
Sbjct: 306 TACLETAK 313
>XP_006713332
          Length = 403

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TACLSTSK 8
           TACL T+K
Sbjct: 306 TACLETAK 313
>XP_006713331
          Length = 403

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TACLSTSK 8
           TACL T+K
Sbjct: 306 TACLETAK 313
>XP_006713330
          Length = 424

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TACLSTSK 8
           TACL T+K
Sbjct: 327 TACLETAK 334
>XP_006713329
          Length = 424

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TACLSTSK 8
           TACL T+K
Sbjct: 327 TACLETAK 334
>XP_005265387
          Length = 426

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TACLSTSK 8
           TACL T+K
Sbjct: 329 TACLETAK 336
>NP_060501
          Length = 1151

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  CLSTSKI 9
          CLSTS+I
Sbjct: 23 CLSTSRI 29
>NP_000284
          Length = 1093

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   CLSTSKI 9
           CL TSKI
Sbjct: 559 CLGTSKI 565
>NP_001027005
          Length = 1086

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   CLSTSKI 9
           CL TSKI
Sbjct: 552 CLGTSKI 558
>NP_079335
          Length = 715

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TACLSTSKI 9
           +A LSTSKI
Sbjct: 112 SAALSTSKI 120
>XP_005256041
          Length = 1086

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   CLSTSKI 9
           CL TSKI
Sbjct: 552 CLGTSKI 558
>XP_005256040
          Length = 1093

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   CLSTSKI 9
           CL TSKI
Sbjct: 559 CLGTSKI 565
>XP_006715631
          Length = 648

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TACLSTSKI 9
           +A LSTSKI
Sbjct: 112 SAALSTSKI 120
>XP_006710061
          Length = 298

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TACLST 6
           TACLST
Sbjct: 267 TACLST 272
>XP_006710060
          Length = 425

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TACLST 6
           TACLST
Sbjct: 394 TACLST 399
>NP_003238
          Length = 1172

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   CLSTSKI 9
           C STSK+
Sbjct: 601 CFSTSKV 607
>NP_689594
          Length = 3549

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TACLSTSK 8
           T+C+STS+
Sbjct: 412 TSCVSTSQ 419
>XP_006712319
          Length = 3308

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TACLSTSK 8
           T+C+STS+
Sbjct: 171 TSCVSTSQ 178
>NP_001186073
          Length = 3327

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TACLSTSK 8
           T+C+STS+
Sbjct: 190 TSCVSTSQ 197
>NP_003822
          Length = 519

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TACLSTSK 8
           T C+ST K
Sbjct: 254 TGCISTGK 261
>NP_002833
          Length = 1115

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   TACLSTS 7
           T+C+STS
Sbjct: 671 TSCVSTS 677
>NP_001154912
          Length = 937

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   TACLSTS 7
           T+C+STS
Sbjct: 493 TSCVSTS 499
>NP_055968
          Length = 2039

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TACLSTSKI 9
            +AC STS I
Sbjct: 1356 SACASTSHI 1364
>NP_001277188
          Length = 1951

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TACLSTSKI 9
            +AC STS I
Sbjct: 1268 SACASTSHI 1276
>NP_009016
          Length = 308

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  ACLSTSKI 9
          ACL+ SKI
Sbjct: 83 ACLTGSKI 90
>NP_065831
          Length = 1392

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TACLST 6
           TAC+ST
Sbjct: 885 TACMST 890
>XP_006723375
          Length = 1137

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   TACLSTS 7
           T+C+STS
Sbjct: 693 TSCVSTS 699
>XP_006715490
          Length = 1181

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TACLSTSKI 9
           +AC STS I
Sbjct: 498 SACASTSHI 506
>XP_005248760
          Length = 1308

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TACLSTSKI 9
           +AC STS I
Sbjct: 625 SACASTSHI 633
>XP_005248759
          Length = 1951

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TACLSTSKI 9
            +AC STS I
Sbjct: 1268 SACASTSHI 1276
>XP_006715489
          Length = 2039

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TACLSTSKI 9
            +AC STS I
Sbjct: 1356 SACASTSHI 1364
>XP_005248758
          Length = 2039

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TACLSTSKI 9
            +AC STS I
Sbjct: 1356 SACASTSHI 1364
>XP_006711904
          Length = 630

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  TACLSTSK 8
          ++CLSTS+
Sbjct: 37 SSCLSTSQ 44
>NP_001026
          Length = 4967

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CLSTSK 8
           CLSTS+
Sbjct: 132 CLSTSR 137
>NP_061027
          Length = 4599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  ACLSTSK 8
          ACL T+K
Sbjct: 83 ACLGTNK 89
>NP_115518
          Length = 2537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    ACLSTSKI 9
            ACLST  +
Sbjct: 2400 ACLSTDSL 2407
>NP_057058
          Length = 319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TACLSTSKI 9
           T+CL  SK+
Sbjct: 123 TSCLMPSKL 131
>XP_006722394
          Length = 1845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    ACLSTSKI 9
            ACLST  +
Sbjct: 1707 ACLSTDSL 1714
>XP_005258167
          Length = 1856

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    ACLSTSKI 9
            ACLST  +
Sbjct: 1718 ACLSTDSL 1725
>XP_006722393
          Length = 2199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    ACLSTSKI 9
            ACLST  +
Sbjct: 2061 ACLSTDSL 2068
>XP_006722392
          Length = 2444

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    ACLSTSKI 9
            ACLST  +
Sbjct: 2306 ACLSTDSL 2313
>XP_006722391
          Length = 2448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    ACLSTSKI 9
            ACLST  +
Sbjct: 2310 ACLSTDSL 2317
>XP_006722390
          Length = 2467

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    ACLSTSKI 9
            ACLST  +
Sbjct: 2330 ACLSTDSL 2337
>XP_005258166
          Length = 2468

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    ACLSTSKI 9
            ACLST  +
Sbjct: 2330 ACLSTDSL 2337
>XP_006722389
          Length = 2506

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    ACLSTSKI 9
            ACLST  +
Sbjct: 2400 ACLSTDSL 2407
>XP_005258165
          Length = 2534

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    ACLSTSKI 9
            ACLST  +
Sbjct: 2396 ACLSTDSL 2403
>XP_005258164
          Length = 2538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    ACLSTSKI 9
            ACLST  +
Sbjct: 2400 ACLSTDSL 2407
>XP_005266473
          Length = 217

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   TACLSTSKI 9
           T+CL  SK+
Sbjct: 123 TSCLMPSKL 131
>XP_006711873
          Length = 4954

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CLSTSK 8
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711872
          Length = 2735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CLSTSK 8
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711871
          Length = 4906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CLSTSK 8
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711870
          Length = 4965

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CLSTSK 8
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711869
          Length = 4973

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CLSTSK 8
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711868
          Length = 4975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CLSTSK 8
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711867
          Length = 4977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CLSTSK 8
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711866
          Length = 4984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CLSTSK 8
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711865
          Length = 4985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CLSTSK 8
           CLSTS+
Sbjct: 132 CLSTSR 137
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.114    0.317 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 578,461
Number of extensions: 531
Number of successful extensions: 63
Number of sequences better than 10000.0: 63
Number of HSP's gapped: 63
Number of HSP's successfully gapped: 63
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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