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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              18    1135
XP_006715292                                                           18    1135
XP_005246407                                                           17    4312
XP_006712401                                                           17    4312
XP_006712400                                                           17    4312
NP_055817                                                              16    5631
NP_296373                                                              16    5631
NP_001849                                                              15    9605

>NP_115927
          Length = 1321

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TAAMFRVVN 9
           TAAMF VVN
Sbjct: 334 TAAMFGVVN 342



>XP_006715292
          Length = 759

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TAAMFRVVN 9
           TAAMF VVN
Sbjct: 321 TAAMFGVVN 329



>XP_005246407
          Length = 462

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  TAAMFRVVN 9
          +AA+F VVN
Sbjct: 35 SAALFNVVN 43



>XP_006712401
          Length = 369

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  TAAMFRVVN 9
          +AA+F VVN
Sbjct: 35 SAALFNVVN 43



>XP_006712400
          Length = 408

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  TAAMFRVVN 9
          +AA+F VVN
Sbjct: 35 SAALFNVVN 43



>NP_055817
          Length = 621

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TAAMFRVVN 9
           TAA++ VVN
Sbjct: 372 TAALYGVVN 380



>NP_296373
          Length = 628

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TAAMFRVVN 9
           TAA++ VVN
Sbjct: 372 TAALYGVVN 380



>NP_001849
          Length = 1142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AAMFRV 7
           AAMFRV
Sbjct: 103 AAMFRV 108



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.340    0.131    0.351 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 208,359
Number of extensions: 122
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)