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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001257436                                                           18    1935
NP_001257437                                                           18    1935
NP_006281                                                              18    1935
XP_006715618                                                           18    1935
XP_005267176                                                           18    1935
NP_001035194                                                           17    4312
NP_055527                                                              15    9605
NP_612361                                                              15    9605
XP_006721170                                                           15    9605
XP_006721169                                                           15    9605
XP_005255780                                                           15    9605

>NP_001257436
          Length = 790

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TAAEASSSL 9
           T AEASSSL
Sbjct: 543 TTAEASSSL 551



>NP_001257437
          Length = 790

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TAAEASSSL 9
           T AEASSSL
Sbjct: 543 TTAEASSSL 551



>NP_006281
          Length = 790

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TAAEASSSL 9
           T AEASSSL
Sbjct: 543 TTAEASSSL 551



>XP_006715618
          Length = 577

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TAAEASSSL 9
           T AEASSSL
Sbjct: 330 TTAEASSSL 338



>XP_005267176
          Length = 790

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TAAEASSSL 9
           T AEASSSL
Sbjct: 543 TTAEASSSL 551



>NP_001035194
          Length = 4493

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1    TAAEASSS 8
            T+AEASSS
Sbjct: 2725 TSAEASSS 2732




 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1    TAAEASSS 8
            T+AEASSS
Sbjct: 2961 TSAEASSS 2968



>NP_055527
          Length = 1707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TAAEASSS 8
           TAA ASSS
Sbjct: 331 TAATASSS 338



>NP_612361
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  TAAEASSS 8
          TAA ASSS
Sbjct: 18 TAAAASSS 25



>XP_006721170
          Length = 1707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TAAEASSS 8
           TAA ASSS
Sbjct: 331 TAATASSS 338



>XP_006721169
          Length = 1707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TAAEASSS 8
           TAA ASSS
Sbjct: 331 TAATASSS 338



>XP_005255780
          Length = 1707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TAAEASSS 8
           TAA ASSS
Sbjct: 331 TAATASSS 338



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.272   0.0829    0.164 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 221,508
Number of extensions: 190
Number of successful extensions: 12
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.6 bits)
S2: 28 (15.4 bits)