Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              21     229
XP_006713721                                                           21     229
NP_056312                                                              17    4312
NP_001273416                                                           17    4312
XP_006720531                                                           17    4312
XP_005254344                                                           17    4312
NP_115495                                                              16    5631
NP_001005499                                                           16    5631
NP_054815                                                              16    7355
NP_001265135                                                           16    7355
NP_001265130                                                           16    7355
NP_060081                                                              16    7355
NP_955361                                                              16    7355
NP_057422                                                              15    9605
NP_001138432                                                           15    9605
NP_001128251                                                           15    9605

>NP_006209
          Length = 1068

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  SYIFISVTQ 9
          SYIF+SVTQ
Sbjct: 67 SYIFVSVTQ 75



>XP_006713721
          Length = 1068

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  SYIFISVTQ 9
          SYIF+SVTQ
Sbjct: 67 SYIFVSVTQ 75



>NP_056312
          Length = 555

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   YIFISVTQ 9
           +IFIS+TQ
Sbjct: 377 WIFISLTQ 384



>NP_001273416
          Length = 494

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   YIFISVTQ 9
           +IFIS+TQ
Sbjct: 316 WIFISLTQ 323



>XP_006720531
          Length = 415

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   YIFISVTQ 9
           +IFIS+TQ
Sbjct: 237 WIFISLTQ 244



>XP_005254344
          Length = 556

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   YIFISVTQ 9
           +IFIS+TQ
Sbjct: 378 WIFISLTQ 385



>NP_115495
          Length = 6306

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    YIFISVT 8
            YIFI++T
Sbjct: 1787 YIFINIT 1793



>NP_001005499
          Length = 312

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YIFISVTQ 9
           YIF+ VT+
Sbjct: 102 YIFLGVTE 109



>NP_054815
          Length = 491

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SYIFISV 7
           S+IFISV
Sbjct: 189 SFIFISV 195



>NP_001265135
          Length = 504

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SYIFISV 7
           S+IFISV
Sbjct: 202 SFIFISV 208



>NP_001265130
          Length = 491

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SYIFISV 7
           S+IFISV
Sbjct: 189 SFIFISV 195



>NP_060081
          Length = 491

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SYIFISV 7
           S+IFISV
Sbjct: 189 SFIFISV 195



>NP_955361
          Length = 491

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SYIFISV 7
           S+IFISV
Sbjct: 189 SFIFISV 195



>NP_057422
          Length = 975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YIFISVTQ 9
           YIF+S T+
Sbjct: 713 YIFLSSTE 720



>NP_001138432
          Length = 544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  YIFISVTQ 9
          Y+F +VTQ
Sbjct: 86 YVFNAVTQ 93



>NP_001128251
          Length = 1015

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YIFISVTQ 9
           YIF+S T+
Sbjct: 753 YIFLSSTE 760



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.137    0.359 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 335,860
Number of extensions: 448
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)