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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              19     665
NP_001127854                                                           16    5631
NP_055684                                                              16    5631
NP_001073583                                                           16    7355
NP_001073585                                                           16    7355
NP_001171811                                                           16    7355
NP_001171808                                                           16    7355
NP_003865                                                              16    7355
NP_001171810                                                           16    7355
XP_005269522                                                           16    7355
NP_057191                                                              15    9605
NP_001018054                                                           15    9605
NP_079328                                                              15    9605
XP_006714540                                                           15    9605
XP_006712837                                                           15    9605

>NP_000312
          Length = 928

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   VNSTANAE 9
           VNSTANAE
Sbjct: 622 VNSTANAE 629



>NP_001127854
          Length = 1114

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SVNSTANA 8
           S+NST+N+
Sbjct: 458 SINSTSNS 465



>NP_055684
          Length = 963

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SVNSTANA 8
           S+NST+N+
Sbjct: 472 SINSTSNS 479



>NP_001073583
          Length = 617

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VNSTANAE 9
           VNS ANA+
Sbjct: 110 VNSPANAD 117



>NP_001073585
          Length = 283

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VNSTANAE 9
           VNS ANA+
Sbjct: 110 VNSPANAD 117



>NP_001171811
          Length = 214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  SVNSTAN 7
          SVNST N
Sbjct: 30 SVNSTCN 36



>NP_001171808
          Length = 345

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SVNSTAN 7
           SVNST N
Sbjct: 144 SVNSTCN 150



>NP_003865
          Length = 328

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SVNSTAN 7
           SVNST N
Sbjct: 144 SVNSTCN 150



>NP_001171810
          Length = 272

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SVNSTAN 7
           SVNST N
Sbjct: 144 SVNSTCN 150



>XP_005269522
          Length = 680

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VNSTANAE 9
           VNS ANA+
Sbjct: 110 VNSPANAD 117



>NP_057191
          Length = 1074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVNSTANAE 9
           S+NST+N +
Sbjct: 476 SLNSTSNTK 484



>NP_001018054
          Length = 1215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   VNSTAN 7
           +NSTAN
Sbjct: 540 INSTAN 545



>NP_079328
          Length = 1328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   VNSTAN 7
           +NSTAN
Sbjct: 653 INSTAN 658



>XP_006714540
          Length = 1030

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVNSTANAE 9
           S+NST+N +
Sbjct: 432 SLNSTSNTK 440



>XP_006712837
          Length = 1328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   VNSTAN 7
           +NSTAN
Sbjct: 653 INSTAN 658



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.273   0.0878    0.192 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 292,043
Number of extensions: 420
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.5 bits)
S2: 28 (15.4 bits)