Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           19     869
NP_004561                                                              19     869
NP_001275703                                                           19     869
XP_005253450                                                           19     869
XP_006719151                                                           19     869
NP_112224                                                              17    4312
NP_689914                                                              17    4312
XP_005253212                                                           17    4312
XP_005253211                                                           17    4312
NP_751945                                                              16    5631
NP_001029288                                                           16    5631
NP_056451                                                              16    5631
XP_006712195                                                           16    5631
XP_005264689                                                           16    5631
NP_065433                                                              16    7355
NP_001123380                                                           16    7355
NP_065749                                                              16    7355
NP_001014980                                                           16    7355
XP_005269098                                                           16    7355
XP_005269097                                                           16    7355
XP_005269096                                                           16    7355
NP_775184                                                              15    9605
NP_115785                                                              15    9605
NP_004804                                                              15    9605
NP_476515                                                              15    9605
NP_002159                                                              15    9605
NP_001269316                                                           15    9605
NP_001277043                                                           15    9605
NP_005887                                                              15    9605
NP_001269315                                                           15    9605
NP_001276839                                                           15    9605
NP_071942                                                              15    9605
NP_115945                                                              15    9605
NP_699197                                                              15    9605
NP_001004485                                                           15    9605
>NP_001275701
          Length = 1486

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SVLVCVETK 9
           SVL CVETK
Sbjct: 439 SVLGCVETK 447
>NP_004561
          Length = 1445

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SVLVCVETK 9
           SVL CVETK
Sbjct: 439 SVLGCVETK 447
>NP_001275703
          Length = 1264

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SVLVCVETK 9
           SVL CVETK
Sbjct: 217 SVLGCVETK 225
>XP_005253450
          Length = 1486

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SVLVCVETK 9
           SVL CVETK
Sbjct: 439 SVLGCVETK 447
>XP_006719151
          Length = 1486

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SVLVCVETK 9
           SVL CVETK
Sbjct: 439 SVLGCVETK 447
>NP_112224
          Length = 1849

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    SVLVCVE 7
            SVLVC+E
Sbjct: 1405 SVLVCLE 1411
>NP_689914
          Length = 5058

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLVCVET 8
           VL+C+ET
Sbjct: 465 VLICLET 471
>XP_005253212
          Length = 1838

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    SVLVCVE 7
            SVLVC+E
Sbjct: 1394 SVLVCLE 1400
>XP_005253211
          Length = 1881

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    SVLVCVE 7
            SVLVC+E
Sbjct: 1437 SVLVCLE 1443
>NP_751945
          Length = 543

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   VLVCVET 8
           VLVC ET
Sbjct: 454 VLVCCET 460
>NP_001029288
          Length = 523

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   VLVCVET 8
           VLVC ET
Sbjct: 434 VLVCCET 440
>NP_056451
          Length = 522

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   VLVCVET 8
           VLVC ET
Sbjct: 433 VLVCCET 439
>XP_006712195
          Length = 507

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   VLVCVET 8
           VLVC ET
Sbjct: 418 VLVCCET 424
>XP_005264689
          Length = 508

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   VLVCVET 8
           VLVC ET
Sbjct: 419 VLVCCET 425
>NP_065433
          Length = 1091

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   VLVCVET 8
           +LVC+E+
Sbjct: 94  ILVCIES 100
>NP_001123380
          Length = 575

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  SVLVCVE 7
          S+LVC E
Sbjct: 87 SILVCAE 93
>NP_065749
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   VLVCVET 8
           +LVCV T
Sbjct: 425 ILVCVST 431
>NP_001014980
          Length = 302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   VLVCVET 8
           VL+C+E+
Sbjct: 232 VLICIES 238
>XP_005269098
          Length = 446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   VLVCVET 8
           +LVCV T
Sbjct: 394 ILVCVST 400
>XP_005269097
          Length = 446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   VLVCVET 8
           +LVCV T
Sbjct: 394 ILVCVST 400
>XP_005269096
          Length = 446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   VLVCVET 8
           +LVCV T
Sbjct: 394 ILVCVST 400
>NP_775184
          Length = 152

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  LVCVETK 9
          L CVET+
Sbjct: 68 LACVETE 74
>NP_115785
          Length = 581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   CVETK 9
           CVETK
Sbjct: 549 CVETK 553
>NP_004804
          Length = 336

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  SVLVCVE 7
          SVL CVE
Sbjct: 87 SVLECVE 93
>NP_476515
          Length = 346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  SVLVCVE 7
          SVL CVE
Sbjct: 87 SVLECVE 93
>NP_002159
          Length = 452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SVLVCVETK 9
           SVLVC + K
Sbjct: 332 SVLVCPDGK 340
>NP_001269316
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SVLVCVETK 9
           SVLVC + K
Sbjct: 293 SVLVCPDGK 301
>NP_001277043
          Length = 322

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SVLVCVETK 9
           SVLVC + K
Sbjct: 202 SVLVCPDGK 210
>NP_005887
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SVLVCVETK 9
           SVLVC + K
Sbjct: 293 SVLVCPDGK 301
>NP_001269315
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SVLVCVETK 9
           SVLVC + K
Sbjct: 293 SVLVCPDGK 301
>NP_001276839
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SVLVCVETK 9
           SVLVC + K
Sbjct: 280 SVLVCPDGK 288
>NP_071942
          Length = 173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  VLVCVETK 9
          VLV VETK
Sbjct: 86 VLVDVETK 93
>NP_115945
          Length = 152

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  LVCVETK 9
          L CVET+
Sbjct: 68 LACVETE 74
>NP_699197
          Length = 3571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    SVLVCVET 8
            S L C+ET
Sbjct: 2534 SALTCLET 2541
>NP_001004485
          Length = 319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LVCVET 8
           LVCV+T
Sbjct: 187 LVCVDT 192
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.129    0.359 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 636,061
Number of extensions: 813
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package