Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              18    1482
NP_009297                                                              18    1482
NP_057090                                                              17    3301
NP_001035282                                                           17    3301
NP_060303                                                              17    3301
XP_006725461                                                           17    3301
XP_006724090                                                           17    3301
NP_000203                                                              17    4312
NP_055847                                                              16    5631
XP_005266355                                                           16    5631
XP_005266354                                                           16    5631
XP_005266353                                                           16    5631
XP_005266352                                                           16    5631
NP_001804                                                              16    7355
NP_958441                                                              16    7355
NP_009148                                                              16    7355
NP_958439                                                              16    7355
NP_665858                                                              16    7355
NP_665859                                                              16    7355
NP_005219                                                              16    7355
NP_001273663                                                           16    7355
NP_848555                                                              16    7355
XP_005260719                                                           16    7355
XP_006723605                                                           16    7355
XP_006723604                                                           16    7355
XP_005247145                                                           16    7355
XP_005247144                                                           16    7355
XP_005247143                                                           16    7355
XP_006713548                                                           16    7355
NP_212134                                                              15    9605
NP_001238892                                                           15    9605
NP_001238891                                                           15    9605
NP_056410                                                              15    9605
NP_653259                                                              15    9605
NP_001113                                                              15    9605
NP_775961                                                              15    9605
NP_001138349                                                           15    9605
XP_006725061                                                           15    9605
XP_005262426                                                           15    9605
XP_005262425                                                           15    9605
XP_006723601                                                           15    9605
XP_005260712                                                           15    9605
XP_006716782                                                           15    9605
>NP_005148
          Length = 1130

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SVKEISDTV 9
            SVKEISD V
Sbjct: 1120 SVKEISDIV 1128
>NP_009297
          Length = 1149

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SVKEISDTV 9
            SVKEISD V
Sbjct: 1139 SVKEISDIV 1147
>NP_057090
          Length = 349

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VKEISDTV 9
           VKEI DTV
Sbjct: 341 VKEICDTV 348
>NP_001035282
          Length = 388

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KEISDTV 9
           KEISDT+
Sbjct: 261 KEISDTI 267
>NP_060303
          Length = 388

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KEISDTV 9
           KEISDT+
Sbjct: 261 KEISDTI 267
>XP_006725461
          Length = 388

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KEISDTV 9
           KEISDT+
Sbjct: 261 KEISDTI 267
>XP_006724090
          Length = 388

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KEISDTV 9
           KEISDT+
Sbjct: 261 KEISDTI 267
>NP_000203
          Length = 788

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SVKEISDT 8
           SVKE+ DT
Sbjct: 667 SVKELKDT 674
>NP_055847
          Length = 1447

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VKEISDTV 9
          VKEISD +
Sbjct: 19 VKEISDKI 26
>XP_005266355
          Length = 1448

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VKEISDTV 9
          VKEISD +
Sbjct: 19 VKEISDKI 26
>XP_005266354
          Length = 1411

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VKEISDTV 9
          VKEISD +
Sbjct: 19 VKEISDKI 26
>XP_005266353
          Length = 1445

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VKEISDTV 9
          VKEISD +
Sbjct: 19 VKEISDKI 26
>XP_005266352
          Length = 1446

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VKEISDTV 9
          VKEISD +
Sbjct: 19 VKEISDKI 26
>NP_001804
          Length = 2701

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    SVKEISDT 8
            +VKE+S+T
Sbjct: 1294 NVKEVSET 1301
>NP_958441
          Length = 705

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SVKEISD 7
           S+KEISD
Sbjct: 452 SLKEISD 458
>NP_009148
          Length = 212

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVKEISDTV 9
           ++KE+ DTV
Sbjct: 137 AIKELLDTV 145
>NP_958439
          Length = 628

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SVKEISD 7
           S+KEISD
Sbjct: 452 SLKEISD 458
>NP_665858
          Length = 212

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVKEISDTV 9
           ++KE+ DTV
Sbjct: 137 AIKELLDTV 145
>NP_665859
          Length = 212

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVKEISDTV 9
           ++KE+ DTV
Sbjct: 137 AIKELLDTV 145
>NP_005219
          Length = 1210

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SVKEISD 7
           S+KEISD
Sbjct: 452 SLKEISD 458
>NP_001273663
          Length = 2580

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    SVKEISDT 8
            +VKE+S+T
Sbjct: 1269 NVKEVSET 1276
>NP_848555
          Length = 197

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  VKEISDT 8
          VKEI DT
Sbjct: 86 VKEIGDT 92
>XP_005260719
          Length = 145

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  VKEISDT 8
          VKEI DT
Sbjct: 86 VKEIGDT 92
>XP_006723605
          Length = 176

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  VKEISDT 8
          VKEI DT
Sbjct: 64 VKEIGDT 70
>XP_006723604
          Length = 198

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  VKEISDT 8
          VKEI DT
Sbjct: 86 VKEIGDT 92
>XP_005247145
          Length = 212

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVKEISDTV 9
           ++KE+ DTV
Sbjct: 137 AIKELLDTV 145
>XP_005247144
          Length = 212

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVKEISDTV 9
           ++KE+ DTV
Sbjct: 137 AIKELLDTV 145
>XP_005247143
          Length = 212

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVKEISDTV 9
           ++KE+ DTV
Sbjct: 137 AIKELLDTV 145
>XP_006713548
          Length = 212

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVKEISDTV 9
           ++KE+ DTV
Sbjct: 137 AIKELLDTV 145
>NP_212134
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SVKEISDTV 9
           +V EISD+V
Sbjct: 168 NVTEISDSV 176
>NP_001238892
          Length = 791

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEISDTV 9
           KE+SDT+
Sbjct: 469 KELSDTM 475
>NP_001238891
          Length = 871

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEISDTV 9
           KE+SDT+
Sbjct: 469 KELSDTM 475
>NP_056410
          Length = 871

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEISDTV 9
           KE+SDT+
Sbjct: 469 KELSDTM 475
>NP_653259
          Length = 2073

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    VKEISDTV 9
            VKE+SD +
Sbjct: 2024 VKELSDII 2031
>NP_001113
          Length = 437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVKEISDTV 9
           S KE+SD++
Sbjct: 362 SFKEVSDSL 370
>NP_775961
          Length = 629

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VKEISDTV 9
           VKE+SD +
Sbjct: 225 VKELSDEI 232
>NP_001138349
          Length = 606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VKEISDTV 9
           VKE+SD +
Sbjct: 202 VKELSDEI 209
>XP_006725061
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEISDTV 9
           KE+SDT+
Sbjct: 469 KELSDTM 475
>XP_005262426
          Length = 2055

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    VKEISDTV 9
            VKE+SD +
Sbjct: 2006 VKELSDII 2013
>XP_005262425
          Length = 2086

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    VKEISDTV 9
            VKE+SD +
Sbjct: 2037 VKELSDII 2044
>XP_006723601
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SVKEISDTV 9
           +V EISD+V
Sbjct: 168 NVTEISDSV 176
>XP_005260712
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SVKEISDTV 9
           +V EISD+V
Sbjct: 168 NVTEISDSV 176
>XP_006716782
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVKEISDTV 9
           S KE+SD++
Sbjct: 362 SFKEVSDSL 370
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.296    0.115    0.257 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 453,237
Number of extensions: 766
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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