Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              20     509
XP_006714302                                                           17    2528
XP_005263127                                                           17    2528
NP_061908                                                              17    2528
NP_001128360                                                           17    3301
NP_004550                                                              16    5631
NP_056162                                                              16    5631
NP_001138898                                                           16    5631
NP_056460                                                              16    5631
NP_689860                                                              16    5631
NP_001265360                                                           16    5631
NP_003642                                                              16    5631
XP_005254276                                                           16    5631
XP_005254275                                                           16    5631
XP_006720491                                                           16    5631
XP_006720490                                                           16    5631
XP_006720489                                                           16    5631
XP_006720488                                                           16    5631
XP_005254273                                                           16    5631
XP_006720487                                                           16    5631
XP_005254272                                                           16    5631
XP_006720486                                                           16    5631
XP_005254271                                                           16    5631
XP_005254270                                                           16    5631
NP_057066                                                              16    5631
NP_001449                                                              16    7355
NP_001120959                                                           16    7355
NP_705838                                                              16    7355
NP_775099                                                              16    7355
NP_056472                                                              16    7355
NP_002544                                                              16    7355
XP_006715973                                                           16    7355
NP_073619                                                              15    9605
NP_005727                                                              15    9605
NP_689801                                                              15    9605
XP_005268737                                                           15    9605
XP_006719250                                                           15    9605
>NP_004251
          Length = 1208

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SVEFDIVKL 9
           SVEFD+VKL
Sbjct: 984 SVEFDMVKL 992
>XP_006714302
          Length = 916

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVEFDIVKL 9
           S+EFD++ L
Sbjct: 105 SIEFDVITL 113
>XP_005263127
          Length = 1134

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVEFDIVKL 9
           S+EFD++ L
Sbjct: 105 SIEFDVITL 113
>NP_061908
          Length = 1135

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVEFDIVKL 9
           S+EFD++ L
Sbjct: 105 SIEFDVITL 113
>NP_001128360
          Length = 1358

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SVEFDIV 7
           S+EFDIV
Sbjct: 489 SIEFDIV 495
>NP_004550
          Length = 324

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SVEFDIVK 8
           +VEFD+V+
Sbjct: 108 TVEFDVVE 115
>NP_056162
          Length = 1052

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EFDIVK 8
           EFDIVK
Sbjct: 428 EFDIVK 433
>NP_001138898
          Length = 303

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SVEFDIVK 8
           +VEFD+V+
Sbjct: 140 TVEFDVVE 147
>NP_056460
          Length = 243

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SVEFDIVK 8
           S++FD+VK
Sbjct: 163 SLQFDLVK 170
>NP_689860
          Length = 508

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   SVEFDIVKL 9
           S++F+I+K+
Sbjct: 338 SIDFNIIKI 346
>NP_001265360
          Length = 243

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SVEFDIVK 8
           S++FD+VK
Sbjct: 163 SLQFDLVK 170
>NP_003642
          Length = 372

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SVEFDIVK 8
           +VEFD+V+
Sbjct: 140 TVEFDVVE 147
>XP_005254276
          Length = 380

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   SVEFDIVKL 9
           S++F+I+K+
Sbjct: 338 SIDFNIIKI 346
>XP_005254275
          Length = 399

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   SVEFDIVKL 9
           S++F+I+K+
Sbjct: 204 SIDFNIIKI 212
>XP_006720491
          Length = 433

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   SVEFDIVKL 9
           S++F+I+K+
Sbjct: 238 SIDFNIIKI 246
>XP_006720490
          Length = 450

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   SVEFDIVKL 9
           S++F+I+K+
Sbjct: 255 SIDFNIIKI 263
>XP_006720489
          Length = 486

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   SVEFDIVKL 9
           S++F+I+K+
Sbjct: 291 SIDFNIIKI 299
>XP_006720488
          Length = 492

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   SVEFDIVKL 9
           S++F+I+K+
Sbjct: 338 SIDFNIIKI 346
>XP_005254273
          Length = 499

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   SVEFDIVKL 9
           S++F+I+K+
Sbjct: 304 SIDFNIIKI 312
>XP_006720487
          Length = 508

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   SVEFDIVKL 9
           S++F+I+K+
Sbjct: 338 SIDFNIIKI 346
>XP_005254272
          Length = 521

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   SVEFDIVKL 9
           S++F+I+K+
Sbjct: 326 SIDFNIIKI 334
>XP_006720486
          Length = 533

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   SVEFDIVKL 9
           S++F+I+K+
Sbjct: 338 SIDFNIIKI 346
>XP_005254271
          Length = 533

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   SVEFDIVKL 9
           S++F+I+K+
Sbjct: 338 SIDFNIIKI 346
>XP_005254270
          Length = 533

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   SVEFDIVKL 9
           S++F+I+K+
Sbjct: 338 SIDFNIIKI 346
>NP_057066
          Length = 364

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SVEFDIVK 8
           +VEFD+V+
Sbjct: 143 TVEFDVVE 150
>NP_001449
          Length = 2725

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   VEFDIVK 8
           ++FDI+K
Sbjct: 817 IDFDIIK 823
>NP_001120959
          Length = 2692

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   VEFDIVK 8
           ++FDI+K
Sbjct: 817 IDFDIIK 823
>NP_705838
          Length = 4069

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    EFDIVKL 9
            E+D+VKL
Sbjct: 3105 EYDLVKL 3111
>NP_775099
          Length = 2595

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SVEFDIVKL 9
            + EFD+V+L
Sbjct: 2248 AAEFDLVQL 2256
>NP_056472
          Length = 2277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SVEFDIVKL 9
            + EFD+V+L
Sbjct: 1930 AAEFDLVQL 1938
>NP_002544
          Length = 435

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SVEFDIVK 8
           +V FDI+K
Sbjct: 422 TVNFDIIK 429
>XP_006715973
          Length = 2632

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   VEFDIVK 8
           ++FDI+K
Sbjct: 817 IDFDIIK 823
>NP_073619
          Length = 1183

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3 EFDIVK 8
         EFD+VK
Sbjct: 3 EFDLVK 8
>NP_005727
          Length = 376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SVEFDIVK 8
           S EF+IVK
Sbjct: 208 SSEFEIVK 215
>NP_689801
          Length = 836

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SVEFDIV 7
           S EFD+V
Sbjct: 742 STEFDVV 748
>XP_005268737
          Length = 719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SVEFDIV 7
           S EFD+V
Sbjct: 625 STEFDVV 631
>XP_006719250
          Length = 1141

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3 EFDIVK 8
         EFD+VK
Sbjct: 3 EFDLVK 8
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.146    0.368 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 379,444
Number of extensions: 449
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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