Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     390
XP_005262424                                                           20     390
NP_001274744                                                           18    1482
NP_001120792                                                           18    1482
NP_001274745                                                           18    1482
NP_055662                                                              18    1482
XP_006711752                                                           18    1482
XP_006711751                                                           18    1482
XP_006711750                                                           18    1482
XP_006711749                                                           18    1482
XP_005245731                                                           18    1482
XP_005245730                                                           18    1482
XP_005245729                                                           18    1482
XP_005245727                                                           18    1482
XP_005245726                                                           18    1482
XP_005245725                                                           18    1482
XP_005245724                                                           18    1482
XP_005245715                                                           18    1482
XP_006711748                                                           18    1482
XP_006711747                                                           18    1482
XP_006711746                                                           18    1482
XP_006711745                                                           18    1482
XP_006711744                                                           18    1482
XP_006711743                                                           18    1482
XP_006711742                                                           18    1482
NP_840054                                                              17    2528
XP_005262121                                                           17    2528
NP_443168                                                              17    2528
NP_733793                                                              17    3301
NP_004832                                                              17    3301
NP_001007103                                                           17    3301
NP_115814                                                              17    3301
NP_775871                                                              17    3301
XP_005267218                                                           17    3301
XP_006715641                                                           17    3301
XP_006715640                                                           17    3301
XP_006715639                                                           17    3301
XP_005245679                                                           17    3301
XP_005245678                                                           17    3301
XP_005245677                                                           17    3301
XP_006711697                                                           17    3301
NP_078961                                                              17    4312
XP_006719666                                                           17    4312
NP_694563                                                              17    4312
NP_001188333                                                           16    5631
NP_008921                                                              16    5631
NP_150229                                                              16    5631
NP_036595                                                              16    7355
NP_001276928                                                           16    7355
NP_001276929                                                           16    7355
NP_005375                                                              16    7355
NP_056235                                                              16    7355
NP_115545                                                              16    7355
NP_065868                                                              16    7355
NP_003128                                                              16    7355
XP_006719381                                                           16    7355
XP_006719380                                                           16    7355
XP_005247737                                                           16    7355
XP_006713778                                                           16    7355
NP_852002                                                              16    7355
NP_001156956                                                           16    7355
NP_079506                                                              16    7355
NP_000114                                                              15    9605
NP_002010                                                              15    9605
NP_001153392                                                           15    9605
NP_001153503                                                           15    9605
NP_001153502                                                           15    9605
NP_036284                                                              15    9605
NP_079215                                                              15    9605
NP_001191354                                                           15    9605
XP_006721337                                                           15    9605
XP_006721336                                                           15    9605
XP_005256210                                                           15    9605
XP_006719928                                                           15    9605
XP_006710605                                                           15    9605
NP_078822                                                              15    9605
>NP_722576
          Length = 3080

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           ST GQEFIE
Sbjct: 431 STAGQEFIE 439
>XP_005262424
          Length = 2875

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           ST GQEFIE
Sbjct: 226 STAGQEFIE 234
>NP_001274744
          Length = 976

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 349 STTGSQFLE 357
>NP_001120792
          Length = 983

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 356 STTGSQFLE 364
>NP_001274745
          Length = 1078

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 367 STTGSQFLE 375
>NP_055662
          Length = 1087

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 356 STTGSQFLE 364
>XP_006711752
          Length = 1085

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 356 STTGSQFLE 364
>XP_006711751
          Length = 820

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  STTGQEFIE 9
          STTG +F+E
Sbjct: 16 STTGSQFLE 24
>XP_006711750
          Length = 930

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 126 STTGSQFLE 134
>XP_006711749
          Length = 965

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 161 STTGSQFLE 169
>XP_005245731
          Length = 983

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 356 STTGSQFLE 364
>XP_005245730
          Length = 994

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 367 STTGSQFLE 375
>XP_005245729
          Length = 1067

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 356 STTGSQFLE 364
>XP_005245727
          Length = 1079

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 367 STTGSQFLE 375
>XP_005245726
          Length = 1095

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 367 STTGSQFLE 375
>XP_005245725
          Length = 1096

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 367 STTGSQFLE 375
>XP_005245724
          Length = 1098

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 367 STTGSQFLE 375
>XP_005245715
          Length = 1115

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 367 STTGSQFLE 375
>XP_006711748
          Length = 1160

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 356 STTGSQFLE 364
>XP_006711747
          Length = 1160

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 356 STTGSQFLE 364
>XP_006711746
          Length = 1160

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 356 STTGSQFLE 364
>XP_006711745
          Length = 1170

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 367 STTGSQFLE 375
>XP_006711744
          Length = 1171

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 367 STTGSQFLE 375
>XP_006711743
          Length = 1171

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 367 STTGSQFLE 375
>XP_006711742
          Length = 1171

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STTG +F+E
Sbjct: 367 STTGSQFLE 375
>NP_840054
          Length = 336

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           +TTG EFI+
Sbjct: 309 TTTGAEFID 317
>XP_005262121
          Length = 321

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           +TTG EFI+
Sbjct: 294 TTTGAEFID 302
>NP_443168
          Length = 398

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           +TTG EFI+
Sbjct: 371 TTTGAEFID 379
>NP_733793
          Length = 1280

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           S TG++F+E
Sbjct: 404 SVTGRQFVE 412
>NP_004832
          Length = 1139

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           S TG++F+E
Sbjct: 256 SVTGRQFVE 264
>NP_001007103
          Length = 755

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  STTGQEF 7
          ST+GQEF
Sbjct: 7  STSGQEF 13
>NP_115814
          Length = 780

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  STTGQEF 7
          ST+GQEF
Sbjct: 7  STSGQEF 13
>NP_775871
          Length = 8384

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    TTGQEFIE 9
            TTG+ FIE
Sbjct: 2272 TTGKSFIE 2279
>XP_005267218
          Length = 780

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  STTGQEF 7
          ST+GQEF
Sbjct: 7  STSGQEF 13
>XP_006715641
          Length = 780

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  STTGQEF 7
          ST+GQEF
Sbjct: 7  STSGQEF 13
>XP_006715640
          Length = 780

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  STTGQEF 7
          ST+GQEF
Sbjct: 7  STSGQEF 13
>XP_006715639
          Length = 815

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  STTGQEF 7
          ST+GQEF
Sbjct: 7  STSGQEF 13
>XP_005245679
          Length = 1142

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           S TG++F+E
Sbjct: 256 SVTGRQFVE 264
>XP_005245678
          Length = 1145

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           S TG++F+E
Sbjct: 259 SVTGRQFVE 267
>XP_005245677
          Length = 1173

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           S TG++F+E
Sbjct: 287 SVTGRQFVE 295
>XP_006711697
          Length = 1179

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           S TG++F+E
Sbjct: 296 SVTGRQFVE 304
>NP_078961
          Length = 723

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   STTGQEFI 8
           ST+G EFI
Sbjct: 269 STSGSEFI 276
>XP_006719666
          Length = 657

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   STTGQEFI 8
           ST+G EFI
Sbjct: 203 STSGSEFI 210
>NP_694563
          Length = 502

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           +T+GQ F+E
Sbjct: 116 ATSGQNFLE 124
>NP_001188333
          Length = 281

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GQEFIE 9
           GQEF+E
Sbjct: 209 GQEFVE 214
>NP_008921
          Length = 498

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GQEFIE 9
           GQEF+E
Sbjct: 209 GQEFVE 214
>NP_150229
          Length = 1359

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   STTGQEFIE 9
           S TG EF++
Sbjct: 750 SETGSEFVD 758
>NP_036595
          Length = 856

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   TGQEFIE 9
           TGQE+++
Sbjct: 643 TGQEYVQ 649
>NP_001276928
          Length = 462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  STTGQEFI 8
          STTG+E +
Sbjct: 39 STTGEELL 46
>NP_001276929
          Length = 462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  STTGQEFI 8
          STTG+E +
Sbjct: 39 STTGEELL 46
>NP_005375
          Length = 462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  STTGQEFI 8
          STTG+E +
Sbjct: 39 STTGEELL 46
>NP_056235
          Length = 597

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   STTGQEFI 8
           S TG+EF+
Sbjct: 443 SPTGKEFV 450
>NP_115545
          Length = 780

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TTGQEFIE 9
           TTGQ F++
Sbjct: 368 TTGQLFVQ 375
>NP_065868
          Length = 936

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STT Q+FI+
Sbjct: 150 STTVQKFID 158
>NP_003128
          Length = 655

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   STTGQEFIE 9
           ST GQ F E
Sbjct: 431 STVGQSFSE 439
>XP_006719381
          Length = 582

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   TGQEFIE 9
           TGQE+++
Sbjct: 369 TGQEYVQ 375
>XP_006719380
          Length = 685

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   TGQEFIE 9
           TGQE+++
Sbjct: 472 TGQEYVQ 478
>XP_005247737
          Length = 661

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STT Q+FI+
Sbjct: 128 STTVQKFID 136
>XP_006713778
          Length = 923

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STTGQEFIE 9
           STT Q+FI+
Sbjct: 137 STTVQKFID 145
>NP_852002
          Length = 520

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TTGQEFI 8
           +TGQ+FI
Sbjct: 424 STGQQFI 430
>NP_001156956
          Length = 546

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TTGQEFI 8
           +TGQ+FI
Sbjct: 450 STGQQFI 456
>NP_079506
          Length = 546

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TTGQEFI 8
           +TGQ+FI
Sbjct: 450 STGQQFI 456
>NP_000114
          Length = 1186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   STTGQEFIE 9
           + TGQ F+E
Sbjct: 763 TVTGQMFLE 771
>NP_002010
          Length = 1338

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   STTGQEFIE 9
           S TG+ F+E
Sbjct: 129 SDTGRPFVE 137
>NP_001153392
          Length = 687

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   STTGQEFIE 9
           S TG+ F+E
Sbjct: 129 SDTGRPFVE 137
>NP_001153503
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   STTGQEFIE 9
           S TG+ F+E
Sbjct: 129 SDTGRPFVE 137
>NP_001153502
          Length = 733

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   STTGQEFIE 9
           S TG+ F+E
Sbjct: 129 SDTGRPFVE 137
>NP_036284
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   STTGQEFIE 9
           S TG ++IE
Sbjct: 331 SDTGSQYIE 339
>NP_079215
          Length = 717

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   TGQEFIE 9
           TG+EF++
Sbjct: 323 TGEEFVK 329
>NP_001191354
          Length = 1640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    STTGQEFIE 9
            + TGQ F+E
Sbjct: 1217 TVTGQMFLE 1225
>XP_006721337
          Length = 591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   TGQEFIE 9
           TG+EF++
Sbjct: 197 TGEEFVK 203
>XP_006721336
          Length = 591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   TGQEFIE 9
           TG+EF++
Sbjct: 197 TGEEFVK 203
>XP_005256210
          Length = 716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   TGQEFIE 9
           TG+EF++
Sbjct: 322 TGEEFVK 328
>XP_006719928
          Length = 649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   STTGQEFIE 9
           S+ G EF+E
Sbjct: 493 SSQGSEFLE 501
>XP_006710605
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   STTGQEFIE 9
           S TG ++IE
Sbjct: 331 SDTGSQYIE 339
>NP_078822
          Length = 726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   STTGQEFIE 9
           S+ G EF+E
Sbjct: 570 SSQGSEFLE 578
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.122    0.311 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 581,469
Number of extensions: 919
Number of successful extensions: 76
Number of sequences better than 10000.0: 76
Number of HSP's gapped: 76
Number of HSP's successfully gapped: 76
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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