Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     509
XP_005262424                                                           20     509
NP_001027006                                                           17    2528
NP_001184033                                                           16    5631
NP_005924                                                              16    5631
NP_001243665                                                           16    5631
NP_036578                                                              16    5631
NP_001243663                                                           16    5631
NP_001243664                                                           16    5631
NP_001243662                                                           16    5631
NP_001243661                                                           16    5631
NP_003793                                                              16    5631
XP_006718903                                                           16    5631
XP_006718902                                                           16    5631
NP_006173                                                              16    7355
NP_001014796                                                           16    7355
NP_849144                                                              16    7355
XP_006713662                                                           16    7355
XP_006713661                                                           16    7355
XP_006713660                                                           16    7355
XP_005247434                                                           16    7355
XP_006713659                                                           16    7355
XP_006711408                                                           16    7355
XP_006711407                                                           16    7355
XP_005245278                                                           16    7355
NP_619639                                                              15    9605
NP_733842                                                              15    9605
NP_001269683                                                           15    9605
NP_001184113                                                           15    9605
NP_000435                                                              15    9605
NP_056049                                                              15    9605
NP_001091988                                                           15    9605
NP_002548                                                              15    9605
NP_002510                                                              15    9605
NP_061897                                                              15    9605
XP_006724995                                                           15    9605
XP_005248952                                                           15    9605
XP_005248951                                                           15    9605
XP_005248950                                                           15    9605
XP_005248949                                                           15    9605
XP_006712098                                                           15    9605
XP_006712097                                                           15    9605
>NP_722576
          Length = 3080

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    STNALSFPY 9
            ST ALSFPY
Sbjct: 1181 STYALSFPY 1189
>XP_005262424
          Length = 2875

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   STNALSFPY 9
           ST ALSFPY
Sbjct: 976 STYALSFPY 984
>NP_001027006
          Length = 1149

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    TNALSFP 8
            TN+LSFP
Sbjct: 1096 TNSLSFP 1102
>NP_001184033
          Length = 3972

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    STNALSFPY 9
            S  ALSFP+
Sbjct: 2364 SKEALSFPH 2372
>NP_005924
          Length = 3969

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    STNALSFPY 9
            S  ALSFP+
Sbjct: 2361 SKEALSFPH 2369
>NP_001243665
          Length = 298

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   TNALSFPY 9
           TNA S PY
Sbjct: 195 TNANSIPY 202
>NP_036578
          Length = 361

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   TNALSFPY 9
           TNA S PY
Sbjct: 225 TNANSIPY 232
>NP_001243663
          Length = 339

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   TNALSFPY 9
           TNA S PY
Sbjct: 203 TNANSIPY 210
>NP_001243664
          Length = 331

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   TNALSFPY 9
           TNA S PY
Sbjct: 195 TNANSIPY 202
>NP_001243662
          Length = 341

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   TNALSFPY 9
           TNA S PY
Sbjct: 205 TNANSIPY 212
>NP_001243661
          Length = 369

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   TNALSFPY 9
           TNA S PY
Sbjct: 233 TNANSIPY 240
>NP_003793
          Length = 1938

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   STNALSFPY 9
           STN   FP+
Sbjct: 306 STNPFDFPF 314
>XP_006718903
          Length = 2673

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    STNALSFPY 9
            S  ALSFP+
Sbjct: 1065 SKEALSFPH 1073
>XP_006718902
          Length = 2964

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    STNALSFPY 9
            S  ALSFP+
Sbjct: 1356 SKEALSFPH 1364
>NP_006173
          Length = 855

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   NALSFP 8
           NA+SFP
Sbjct: 316 NAISFP 321
>NP_001014796
          Length = 855

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   NALSFP 8
           NA+SFP
Sbjct: 316 NAISFP 321
>NP_849144
          Length = 2623

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    STNALSFP 8
            +T ALSFP
Sbjct: 1075 ATAALSFP 1082
>XP_006713662
          Length = 1846

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    STNALSFP 8
            +T ALSFP
Sbjct: 1147 ATAALSFP 1154
>XP_006713661
          Length = 1860

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    STNALSFP 8
            +T ALSFP
Sbjct: 1147 ATAALSFP 1154
>XP_006713660
          Length = 2623

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    STNALSFP 8
            +T ALSFP
Sbjct: 1075 ATAALSFP 1082
>XP_005247434
          Length = 2623

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    STNALSFP 8
            +T ALSFP
Sbjct: 1075 ATAALSFP 1082
>XP_006713659
          Length = 2695

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    STNALSFP 8
            +T ALSFP
Sbjct: 1147 ATAALSFP 1154
>XP_006711408
          Length = 571

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   NALSFP 8
           NA+SFP
Sbjct: 328 NAISFP 333
>XP_006711407
          Length = 855

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   NALSFP 8
           NA+SFP
Sbjct: 316 NAISFP 321
>XP_005245278
          Length = 867

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   NALSFP 8
           NA+SFP
Sbjct: 328 NAISFP 333
>NP_619639
          Length = 4074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    STNALSFPY 9
            S+N ++FP+
Sbjct: 2510 SSNLVAFPF 2518
>NP_733842
          Length = 3396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    STNALSFPY 9
            S+N ++FP+
Sbjct: 2510 SSNLVAFPF 2518
>NP_001269683
          Length = 695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   STNALSFP 8
           STN + FP
Sbjct: 544 STNQIRFP 551
>NP_001184113
          Length = 333

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   STNALSFPY 9
           S  ALS PY
Sbjct: 155 SAAALSIPY 163
>NP_000435
          Length = 749

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   STNALSFP 8
           STN + FP
Sbjct: 544 STNQIRFP 551
>NP_056049
          Length = 1346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  NALSFP 8
          N+LSFP
Sbjct: 85 NSLSFP 90
>NP_001091988
          Length = 1346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  NALSFP 8
          N+LSFP
Sbjct: 85 NSLSFP 90
>NP_002548
          Length = 678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NALSFPY 9
           NA+SF Y
Sbjct: 333 NAISFSY 339
>NP_002510
          Length = 1427

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    NALSFP 8
            NA+SFP
Sbjct: 1091 NAVSFP 1096
>NP_061897
          Length = 2071

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    STNALSFPY 9
            S  ALS PY
Sbjct: 1915 SNRALSTPY 1923
>XP_006724995
          Length = 462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NALSFPY 9
           NA+S PY
Sbjct: 430 NAISSPY 436
>XP_005248952
          Length = 1380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  NALSFP 8
          N+LSFP
Sbjct: 85 NSLSFP 90
>XP_005248951
          Length = 1381

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  NALSFP 8
          N+LSFP
Sbjct: 85 NSLSFP 90
>XP_005248950
          Length = 1386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  NALSFP 8
          N+LSFP
Sbjct: 85 NSLSFP 90
>XP_005248949
          Length = 1386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  NALSFP 8
          N+LSFP
Sbjct: 85 NSLSFP 90
>XP_006712098
          Length = 2059

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    STNALSFPY 9
            S  ALS PY
Sbjct: 1903 SNRALSTPY 1911
>XP_006712097
          Length = 2070

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    STNALSFPY 9
            S  ALS PY
Sbjct: 1914 SNRALSTPY 1922
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.123    0.362 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 534,297
Number of extensions: 497
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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