Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              19     869
XP_005267290                                                           19     869
NP_055907                                                              16    7355
XP_006726695                                                           16    7355
XP_005255242                                                           16    7355
XP_005255241                                                           16    7355
XP_005255240                                                           16    7355
XP_005255239                                                           16    7355
NP_005133                                                              15    9605
XP_005273967                                                           15    9605

>NP_005091
          Length = 1782

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  STINSVAEQ 9
          STIN VAEQ
Sbjct: 63 STINGVAEQ 71



>XP_005267290
          Length = 1782

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  STINSVAEQ 9
          STIN VAEQ
Sbjct: 63 STINGVAEQ 71



>NP_055907
          Length = 3661

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    INSVAEQ 9
            INSVA+Q
Sbjct: 1107 INSVAQQ 1113



>XP_006726695
          Length = 747

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   INSVAEQ 9
           INSVA+Q
Sbjct: 622 INSVAQQ 628



>XP_005255242
          Length = 3609

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    INSVAEQ 9
            INSVA+Q
Sbjct: 1055 INSVAQQ 1061



>XP_005255241
          Length = 3637

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    INSVAEQ 9
            INSVA+Q
Sbjct: 1055 INSVAQQ 1061



>XP_005255240
          Length = 3663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    INSVAEQ 9
            INSVA+Q
Sbjct: 1081 INSVAQQ 1087



>XP_005255239
          Length = 3689

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    INSVAEQ 9
            INSVA+Q
Sbjct: 1107 INSVAQQ 1113



>NP_005133
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   STINSVAE 8
           S+IN++AE
Sbjct: 371 SSINNIAE 378



>XP_005273967
          Length = 392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   STINSVAE 8
           S+IN++AE
Sbjct: 346 SSINNIAE 353



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.287   0.0999    0.217 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 275,135
Number of extensions: 271
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)