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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     869
XP_005262424                                                           19     869
NP_001269939                                                           17    3301
NP_001269938                                                           17    3301
NP_116575                                                              17    3301
NP_057518                                                              17    3301
NP_851996                                                              16    7355
NP_001229537                                                           16    7355
NP_001070150                                                           16    7355
NP_001229536                                                           16    7355
NP_000606                                                              16    7355
NP_005568                                                              15    9605
NP_997248                                                              15    9605

>NP_722576
          Length = 3080

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   STAVSQQID 9
           ST VSQQID
Sbjct: 240 STTVSQQID 248



>XP_005262424
          Length = 2875

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  STAVSQQID 9
          ST VSQQID
Sbjct: 35 STTVSQQID 43



>NP_001269939
          Length = 996

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STAVSQQID 9
           STA +QQ+D
Sbjct: 786 STAAAQQLD 794



>NP_001269938
          Length = 1300

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    STAVSQQID 9
            STA +QQ+D
Sbjct: 1009 STAAAQQLD 1017



>NP_116575
          Length = 1243

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    STAVSQQID 9
            STA +QQ+D
Sbjct: 1033 STAAAQQLD 1041



>NP_057518
          Length = 1219

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    STAVSQQID 9
            STA +QQ+D
Sbjct: 1009 STAAAQQLD 1017



>NP_851996
          Length = 858

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  TAVSQQID 9
          TAVS Q+D
Sbjct: 16 TAVSLQVD 23



>NP_001229537
          Length = 726

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  TAVSQQID 9
          TAVS Q+D
Sbjct: 16 TAVSLQVD 23



>NP_001070150
          Length = 761

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  TAVSQQID 9
          TAVS Q+D
Sbjct: 16 TAVSLQVD 23



>NP_001229536
          Length = 884

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  TAVSQQID 9
          TAVS Q+D
Sbjct: 16 TAVSLQVD 23



>NP_000606
          Length = 848

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  TAVSQQID 9
          TAVS Q+D
Sbjct: 16 TAVSLQVD 23



>NP_005568
          Length = 2040

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    STAVSQQ 7
            STAVS+Q
Sbjct: 1256 STAVSEQ 1262



>NP_997248
          Length = 401

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   STAVSQQI 8
           +TAV+QQ+
Sbjct: 195 ATAVTQQV 202



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.297    0.106    0.236 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 240,944
Number of extensions: 122
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)