Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              18    1482
NP_001019849                                                           17    3301
NP_001269266                                                           17    3301
NP_001269267                                                           17    3301
NP_001165806                                                           17    3301
NP_001129497                                                           17    3301
NP_005023                                                              17    3301
NP_006360                                                              15    9605
XP_005272378                                                           15    9605
XP_005270433                                                           15    9605
NP_038460                                                              15    9605

>NP_004295
          Length = 1620

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    SSLAMLDL 9
            SSLAMLDL
Sbjct: 1219 SSLAMLDL 1226



>NP_001019849
          Length = 513

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SSLAMLDL 9
           SSLA+LDL
Sbjct: 170 SSLALLDL 177



>NP_001269266
          Length = 617

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   SSSLAMLDL 9
           SSSLA++DL
Sbjct: 537 SSSLAVVDL 545



>NP_001269267
          Length = 585

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   SSSLAMLDL 9
           SSSLA++DL
Sbjct: 505 SSSLAVVDL 513



>NP_001165806
          Length = 603

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   SSSLAMLDL 9
           SSSLA++DL
Sbjct: 523 SSSLAVVDL 531



>NP_001129497
          Length = 630

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   SSSLAMLDL 9
           SSSLA++DL
Sbjct: 550 SSSLAVVDL 558



>NP_005023
          Length = 630

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   SSSLAMLDL 9
           SSSLA++DL
Sbjct: 550 SSSLAVVDL 558



>NP_006360
          Length = 812

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SSSLAMLDL 9
           SSSL  LD+
Sbjct: 731 SSSLCQLDI 739



>XP_005272378
          Length = 1377

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SLAMLDL 9
            SLA+LDL
Sbjct: 1098 SLALLDL 1104



>XP_005270433
          Length = 820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SSSLAMLDL 9
           SSSL  LD+
Sbjct: 739 SSSLCQLDI 747



>NP_038460
          Length = 1378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SLAMLDL 9
            SLA+LDL
Sbjct: 1099 SLALLDL 1105



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.119    0.265 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 312,887
Number of extensions: 289
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)