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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              18    1135
NP_009297                                                              18    1135
NP_002070                                                              16    5631
NP_114404                                                              16    5631
NP_076413                                                              16    5631
XP_006714556                                                           16    5631
XP_006714555                                                           16    5631
NP_001009877                                                           16    5631
NP_001243084                                                           15    9605
NP_001353                                                              15    9605
NP_079382                                                              15    9605
NP_060644                                                              15    9605

>NP_005148
          Length = 1130

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSRLAIGEE 9
           SSRLA GEE
Sbjct: 695 SSRLATGEE 703



>NP_009297
          Length = 1149

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSRLAIGEE 9
           SSRLA GEE
Sbjct: 714 SSRLATGEE 722



>NP_002070
          Length = 413

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  SSRLAIGEE 9
          +SRLA+G++
Sbjct: 84 ASRLALGDD 92



>NP_114404
          Length = 433

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SRLAIGEE 9
           SRLA+G E
Sbjct: 278 SRLALGSE 285



>NP_076413
          Length = 597

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SRLAIGEE 9
           SRL++GE+
Sbjct: 568 SRLSVGEQ 575



>XP_006714556
          Length = 617

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SRLAIGEE 9
           SRL++GE+
Sbjct: 588 SRLSVGEQ 595



>XP_006714555
          Length = 596

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SRLAIGEE 9
           SRL++GE+
Sbjct: 567 SRLSVGEQ 574



>NP_001009877
          Length = 544

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SRLAIGEE 9
           SRL++GE+
Sbjct: 515 SRLSVGEQ 522



>NP_001243084
          Length = 2290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    SRLAIGEE 9
            SRL  GEE
Sbjct: 1863 SRLGAGEE 1870



>NP_001353
          Length = 304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  SSRLAIGE 8
          +SRL +GE
Sbjct: 6  TSRLVVGE 13



>NP_079382
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SSRLAIGEE 9
           S RLA+G E
Sbjct: 244 SVRLALGRE 252



>NP_060644
          Length = 1059

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SSRLAIGEE 9
           +SRLA GE+
Sbjct: 345 ASRLARGED 353



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.121    0.284 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 392,867
Number of extensions: 471
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)