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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           20     509
NP_004561                                                              20     509
XP_005253450                                                           20     509
XP_006719151                                                           20     509
NP_852125                                                              19     869
NP_780775                                                              17    2528
NP_001230199                                                           17    2528
NP_055176                                                              17    2528
NP_001276900                                                           17    3301
NP_001276901                                                           17    3301
NP_001120844                                                           17    3301
NP_003419                                                              17    3301
XP_006719810                                                           17    3301
XP_005266243                                                           17    3301
XP_005266242                                                           17    3301
NP_653271                                                              16    5631
NP_001269353                                                           16    5631
XP_006722357                                                           16    7355
XP_006723077                                                           15    9605
NP_001073905                                                           15    9605

>NP_001275701
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  SSQVSLGFE 9
          SSQVSLGF+
Sbjct: 33 SSQVSLGFD 41



>NP_004561
          Length = 1445

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  SSQVSLGFE 9
          SSQVSLGF+
Sbjct: 33 SSQVSLGFD 41



>XP_005253450
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  SSQVSLGFE 9
          SSQVSLGF+
Sbjct: 33 SSQVSLGFD 41



>XP_006719151
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  SSQVSLGFE 9
          SSQVSLGF+
Sbjct: 33 SSQVSLGFD 41



>NP_852125
          Length = 407

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   SSQVSLGFE 9
           SSQ+SLGF+
Sbjct: 397 SSQISLGFK 405



>NP_780775
          Length = 1026

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SQVSLGFE 9
           SQVSLG+E
Sbjct: 914 SQVSLGWE 921



>NP_001230199
          Length = 1100

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SQVSLGFE 9
           SQVSLG+E
Sbjct: 988 SQVSLGWE 995



>NP_055176
          Length = 1100

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SQVSLGFE 9
           SQVSLG+E
Sbjct: 988 SQVSLGWE 995



>NP_001276900
          Length = 738

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SSQVSLGFE 9
          SS VSLG+E
Sbjct: 42 SSLVSLGYE 50



>NP_001276901
          Length = 738

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SSQVSLGFE 9
          SS VSLG+E
Sbjct: 42 SSLVSLGYE 50



>NP_001120844
          Length = 738

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SSQVSLGFE 9
          SS VSLG+E
Sbjct: 42 SSLVSLGYE 50



>NP_003419
          Length = 738

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SSQVSLGFE 9
          SS VSLG+E
Sbjct: 42 SSLVSLGYE 50



>XP_006719810
          Length = 738

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SSQVSLGFE 9
          SS VSLG+E
Sbjct: 42 SSLVSLGYE 50



>XP_005266243
          Length = 738

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SSQVSLGFE 9
          SS VSLG+E
Sbjct: 42 SSLVSLGYE 50



>XP_005266242
          Length = 738

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SSQVSLGFE 9
          SS VSLG+E
Sbjct: 42 SSLVSLGYE 50



>NP_653271
          Length = 1454

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SQVSLGF 8
           +QVSLGF
Sbjct: 560 NQVSLGF 566



>NP_001269353
          Length = 963

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  SQVSLGF 8
          +QVSLGF
Sbjct: 69 NQVSLGF 75



>XP_006722357
          Length = 347

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   QVSLGF 8
           QVSLGF
Sbjct: 181 QVSLGF 186



>XP_006723077
          Length = 384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SSQVSLGFE 9
           SS +SLG++
Sbjct: 107 SSGISLGYD 115



>NP_001073905
          Length = 430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SSQVSLGFE 9
           SS +SLG++
Sbjct: 107 SSGISLGYD 115



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.121    0.293 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 354,788
Number of extensions: 251
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)