Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_694543                                                              18    1135
NP_004110                                                              18    1482
NP_004447                                                              17    3301
NP_001190176                                                           17    3301
XP_006715950                                                           17    3301
XP_006715946                                                           17    3301
XP_005250019                                                           17    3301
NP_001140                                                              16    7355
NP_001191332                                                           16    7355
NP_001191333                                                           16    7355
NP_066267                                                              16    7355
NP_001135918                                                           16    7355
NP_065208                                                              16    7355
XP_006717897                                                           16    7355
XP_006717896                                                           16    7355
XP_005269773                                                           16    7355
XP_006717866                                                           16    7355
XP_005269772                                                           16    7355
XP_006717865                                                           16    7355
XP_006717864                                                           16    7355
XP_006717863                                                           16    7355
XP_006717862                                                           16    7355
XP_006717861                                                           16    7355
XP_006717860                                                           16    7355
XP_006717859                                                           16    7355
XP_006717858                                                           16    7355
XP_006717857                                                           16    7355
XP_006717856                                                           16    7355
XP_006717855                                                           16    7355
XP_006717854                                                           16    7355
XP_006717853                                                           16    7355
XP_006717852                                                           16    7355
XP_006717851                                                           16    7355
XP_006717850                                                           16    7355
XP_006717849                                                           16    7355
XP_006717848                                                           16    7355
XP_006717847                                                           16    7355
XP_006717846                                                           16    7355
XP_006717845                                                           16    7355
XP_005273533                                                           16    7355
NP_065210                                                              16    7355
NP_065209                                                              16    7355
NP_000028                                                              16    7355
NP_001254505                                                           15    9605
NP_694942                                                              15    9605
NP_543140                                                              15    9605
NP_001004696                                                           15    9605
>NP_694543
          Length = 707

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1  SSMRGSREV 9
          SS+RG+REV
Sbjct: 75 SSLRGTREV 83
>NP_004110
          Length = 993

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSMRGSREV 9
           SSM GSREV
Sbjct: 735 SSMPGSREV 743
>NP_004447
          Length = 751

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  SSMRGSRE 8
          SS+RG+RE
Sbjct: 75 SSLRGTRE 82
>NP_001190176
          Length = 746

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  SSMRGSRE 8
          SS+RG+RE
Sbjct: 75 SSLRGTRE 82
>XP_006715950
          Length = 712

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  SSMRGSRE 8
          SS+RG+RE
Sbjct: 83 SSLRGTRE 90
>XP_006715946
          Length = 746

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  SSMRGSRE 8
          SS+RG+RE
Sbjct: 75 SSLRGTRE 82
>XP_005250019
          Length = 754

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  SSMRGSRE 8
          SS+RG+RE
Sbjct: 83 SSLRGTRE 90
>NP_001140
          Length = 1001

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SMRGSR 7
           SMRGSR
Sbjct: 130 SMRGSR 135
>NP_001191332
          Length = 1861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SMRGSR 7
           SMRGSR
Sbjct: 990 SMRGSR 995
>NP_001191333
          Length = 1868

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 997  SMRGSR 1002
>NP_066267
          Length = 4377

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 996  SMRGSR 1001
>NP_001135918
          Length = 1897

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SMRGSR 7
           SMRGSR
Sbjct: 966 SMRGSR 971
>NP_065208
          Length = 1856

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SMRGSR 7
           SMRGSR
Sbjct: 925 SMRGSR 930
>XP_006717897
          Length = 1160

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  SSMRGSREV 9
          + M GSRE+
Sbjct: 2  NKMEGSREI 10
>XP_006717896
          Length = 1186

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  SSMRGSREV 9
          + M GSRE+
Sbjct: 28 NKMEGSREI 36
>XP_005269773
          Length = 1867

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 996  SMRGSR 1001
>XP_006717866
          Length = 1877

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1014 SMRGSR 1019
>XP_005269772
          Length = 1885

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1014 SMRGSR 1019
>XP_006717865
          Length = 1898

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1018 SMRGSR 1023
>XP_006717864
          Length = 1924

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1018 SMRGSR 1023
>XP_006717863
          Length = 1972

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1018 SMRGSR 1023
>XP_006717862
          Length = 1981

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1018 SMRGSR 1023
>XP_006717861
          Length = 1984

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1018 SMRGSR 1023
>XP_006717860
          Length = 1993

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1018 SMRGSR 1023
>XP_006717859
          Length = 2758

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1018 SMRGSR 1023
>XP_006717858
          Length = 2775

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1018 SMRGSR 1023
>XP_006717857
          Length = 2794

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1018 SMRGSR 1023
>XP_006717856
          Length = 2833

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1018 SMRGSR 1023
>XP_006717855
          Length = 2837

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1018 SMRGSR 1023
>XP_006717854
          Length = 2852

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1018 SMRGSR 1023
>XP_006717853
          Length = 2854

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1018 SMRGSR 1023
>XP_006717852
          Length = 3508

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SMRGSR 7
           SMRGSR
Sbjct: 125 SMRGSR 130
>XP_006717851
          Length = 3513

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SMRGSR 7
           SMRGSR
Sbjct: 130 SMRGSR 135
>XP_006717850
          Length = 4364

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SMRGSR 7
           SMRGSR
Sbjct: 981 SMRGSR 986
>XP_006717849
          Length = 4383

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1000 SMRGSR 1005
>XP_006717848
          Length = 4392

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1009 SMRGSR 1014
>XP_006717847
          Length = 4395

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1012 SMRGSR 1017
>XP_006717846
          Length = 4396

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1013 SMRGSR 1018
>XP_006717845
          Length = 4397

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SMRGSR 7
            SMRGSR
Sbjct: 1014 SMRGSR 1019
>XP_005273533
          Length = 1921

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SMRGSR 7
           SMRGSR
Sbjct: 966 SMRGSR 971
>NP_065210
          Length = 1719

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SMRGSR 7
           SMRGSR
Sbjct: 925 SMRGSR 930
>NP_065209
          Length = 1881

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SMRGSR 7
           SMRGSR
Sbjct: 925 SMRGSR 930
>NP_000028
          Length = 1880

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SMRGSR 7
           SMRGSR
Sbjct: 925 SMRGSR 930
>NP_001254505
          Length = 1118

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SSMRGS 6
           SSMRGS
Sbjct: 715 SSMRGS 720
>NP_694942
          Length = 1123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SSMRGS 6
           SSMRGS
Sbjct: 720 SSMRGS 725
>NP_543140
          Length = 142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MRGSREV 9
           +RGSR+V
Sbjct: 114 LRGSRDV 120
>NP_001004696
          Length = 348

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RGSREV 9
           RGSRE+
Sbjct: 201 RGSREI 206
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.114    0.271 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 477,705
Number of extensions: 495
Number of successful extensions: 47
Number of sequences better than 10000.0: 47
Number of HSP's gapped: 47
Number of HSP's successfully gapped: 47
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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