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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              18    1135
NP_001075109                                                           18    1135
NP_149110                                                              18    1935
XP_005256268                                                           18    1935
NP_149111                                                              17    4312
NP_001258011                                                           17    4312
XP_006725072                                                           17    4312
XP_006725071                                                           17    4312
XP_006725070                                                           17    4312
XP_005248440                                                           17    4312
XP_006714568                                                           17    4312
XP_005248439                                                           17    4312
NP_056073                                                              16    7355
XP_006717082                                                           16    7355
XP_006717081                                                           16    7355

>NP_008835
          Length = 4128

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    SKLLRMKL 9
            SKLLRMKL
Sbjct: 3649 SKLLRMKL 3656



>NP_001075109
          Length = 4097

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    SKLLRMKL 9
            SKLLRMKL
Sbjct: 3649 SKLLRMKL 3656



>NP_149110
          Length = 470

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   SSKLLRMKL 9
           SSK+LR+KL
Sbjct: 177 SSKMLRVKL 185



>XP_005256268
          Length = 366

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1  SSKLLRMKL 9
          SSK+LR+KL
Sbjct: 73 SSKMLRVKL 81



>NP_149111
          Length = 451

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SSKLLRMKL 9
           SSK LR+KL
Sbjct: 165 SSKTLRVKL 173



>NP_001258011
          Length = 311

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SSKLLRMKL 9
           SSK LR+KL
Sbjct: 165 SSKTLRVKL 173



>XP_006725072
          Length = 360

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SSKLLRMKL 9
           SSK LR+KL
Sbjct: 165 SSKTLRVKL 173



>XP_006725071
          Length = 440

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SSKLLRMKL 9
           SSK LR+KL
Sbjct: 165 SSKTLRVKL 173



>XP_006725070
          Length = 500

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SSKLLRMKL 9
           SSK LR+KL
Sbjct: 165 SSKTLRVKL 173



>XP_005248440
          Length = 360

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SSKLLRMKL 9
           SSK LR+KL
Sbjct: 165 SSKTLRVKL 173



>XP_006714568
          Length = 440

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SSKLLRMKL 9
           SSK LR+KL
Sbjct: 165 SSKTLRVKL 173



>XP_005248439
          Length = 500

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SSKLLRMKL 9
           SSK LR+KL
Sbjct: 165 SSKTLRVKL 173



>NP_056073
          Length = 1219

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KLLRMKL 9
           KLLR+KL
Sbjct: 226 KLLRLKL 232



>XP_006717082
          Length = 1151

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KLLRMKL 9
           KLLR+KL
Sbjct: 158 KLLRLKL 164



>XP_006717081
          Length = 1209

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KLLRMKL 9
           KLLR+KL
Sbjct: 226 KLLRLKL 232



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.130    0.296 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 296,726
Number of extensions: 289
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)