Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005219                                                              18    1135
NP_001276866                                                           18    1135
NP_004439                                                              18    1135
NP_001276865                                                           18    1135
NP_001005862                                                           18    1135
XP_005257197                                                           18    1135
XP_006721828                                                           18    1135
XP_006720036                                                           17    2528
XP_005268376                                                           17    2528
XP_006720034                                                           17    2528
XP_006720033                                                           17    2528
XP_005268375                                                           17    2528
XP_006720032                                                           17    2528
NP_060375                                                              17    2528
NP_056110                                                              16    5631
NP_001263200                                                           16    5631
NP_001155056                                                           16    5631
NP_060216                                                              16    5631
NP_056262                                                              16    5631
NP_001263201                                                           16    5631
NP_003916                                                              16    5631
NP_001263202                                                           16    5631
NP_001263199                                                           16    5631
NP_065937                                                              16    5631
XP_005258159                                                           16    5631
XP_006722384                                                           16    5631
XP_005271696                                                           16    5631
XP_005271695                                                           16    5631
XP_005271694                                                           16    5631
XP_005271693                                                           16    5631
XP_005271690                                                           16    5631
XP_005271689                                                           16    5631
XP_005271688                                                           16    5631
XP_005271687                                                           16    5631
XP_005263454                                                           16    5631
XP_005263453                                                           16    5631
NP_004295                                                              16    7355
NP_071942                                                              16    7355
NP_055625                                                              16    7355
NP_055836                                                              16    7355
NP_055045                                                              16    7355
NP_001247431                                                           16    7355
XP_005248755                                                           16    7355
XP_005248754                                                           16    7355
XP_006711734                                                           16    7355
XP_006711733                                                           16    7355
XP_006711732                                                           16    7355
XP_006711731                                                           16    7355
XP_006711730                                                           16    7355
XP_006711729                                                           16    7355
XP_005245695                                                           16    7355
XP_006711403                                                           16    7355
NP_653300                                                              16    7355
NP_000867                                                              15    9605
NP_002894                                                              15    9605
>NP_005219
          Length = 1210

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSKANKEIL 9
           S KANKEIL
Sbjct: 752 SPKANKEIL 760
>NP_001276866
          Length = 1055

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSKANKEIL 9
           S KANKEIL
Sbjct: 760 SPKANKEIL 768
>NP_004439
          Length = 1255

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSKANKEIL 9
           S KANKEIL
Sbjct: 760 SPKANKEIL 768
>NP_001276865
          Length = 1240

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSKANKEIL 9
           S KANKEIL
Sbjct: 745 SPKANKEIL 753
>NP_001005862
          Length = 1225

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSKANKEIL 9
           S KANKEIL
Sbjct: 730 SPKANKEIL 738
>XP_005257197
          Length = 1225

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSKANKEIL 9
           S KANKEIL
Sbjct: 730 SPKANKEIL 738
>XP_006721828
          Length = 1336

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSKANKEIL 9
           S KANKEIL
Sbjct: 841 SPKANKEIL 849
>XP_006720036
          Length = 296

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SKANKEIL 9
           SK NKEIL
Sbjct: 112 SKGNKEIL 119
>XP_005268376
          Length = 543

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SKANKEIL 9
           SK NKEIL
Sbjct: 493 SKGNKEIL 500
>XP_006720034
          Length = 613

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SKANKEIL 9
           SK NKEIL
Sbjct: 429 SKGNKEIL 436
>XP_006720033
          Length = 646

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SKANKEIL 9
           SK NKEIL
Sbjct: 462 SKGNKEIL 469
>XP_005268375
          Length = 655

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SKANKEIL 9
           SK NKEIL
Sbjct: 493 SKGNKEIL 500
>XP_006720032
          Length = 677

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SKANKEIL 9
           SK NKEIL
Sbjct: 493 SKGNKEIL 500
>NP_060375
          Length = 677

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SKANKEIL 9
           SK NKEIL
Sbjct: 493 SKGNKEIL 500
>NP_056110
          Length = 2005

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SSKANKEIL 9
           SS  NKEI+
Sbjct: 754 SSSGNKEII 762
>NP_001263200
          Length = 572

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SSKANKEIL 9
           SSK NKE L
Sbjct: 419 SSKYNKEAL 427
>NP_001155056
          Length = 913

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SSKANKEIL 9
           +++ANKE+L
Sbjct: 584 AARANKELL 592
>NP_060216
          Length = 929

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SSKANKEIL 9
           +++ANKE+L
Sbjct: 581 AARANKELL 589
>NP_056262
          Length = 1602

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SSKANKEIL 9
            SSK+NK I+
Sbjct: 1471 SSKSNKPII 1479
>NP_001263201
          Length = 540

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SSKANKEIL 9
           SSK NKE L
Sbjct: 419 SSKYNKEAL 427
>NP_003916
          Length = 580

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SSKANKEIL 9
           SSK NKE L
Sbjct: 419 SSKYNKEAL 427
>NP_001263202
          Length = 262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SSKANKEIL 9
           SSK NKE L
Sbjct: 101 SSKYNKEAL 109
>NP_001263199
          Length = 574

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SSKANKEIL 9
           SSK NKE L
Sbjct: 413 SSKYNKEAL 421
>NP_065937
          Length = 1077

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SKANKEIL 9
          S ANKE+L
Sbjct: 81 SAANKEVL 88
>XP_005258159
          Length = 1817

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SSKANKEIL 9
           SS  NKEI+
Sbjct: 566 SSSGNKEII 574
>XP_006722384
          Length = 1877

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SSKANKEIL 9
           SS  NKEI+
Sbjct: 626 SSSGNKEII 634
>XP_005271696
          Length = 1045

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SKANKEIL 9
          S ANKE+L
Sbjct: 81 SAANKEVL 88
>XP_005271695
          Length = 1046

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SKANKEIL 9
          S ANKE+L
Sbjct: 81 SAANKEVL 88
>XP_005271694
          Length = 1078

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SKANKEIL 9
          S ANKE+L
Sbjct: 81 SAANKEVL 88
>XP_005271693
          Length = 1080

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SKANKEIL 9
          S ANKE+L
Sbjct: 81 SAANKEVL 88
>XP_005271690
          Length = 1118

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SKANKEIL 9
          S ANKE+L
Sbjct: 59 SAANKEVL 66
>XP_005271689
          Length = 1137

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SKANKEIL 9
          S ANKE+L
Sbjct: 78 SAANKEVL 85
>XP_005271688
          Length = 1139

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SKANKEIL 9
          S ANKE+L
Sbjct: 81 SAANKEVL 88
>XP_005271687
          Length = 1140

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SKANKEIL 9
          S ANKE+L
Sbjct: 81 SAANKEVL 88
>XP_005263454
          Length = 1324

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SSKANKEIL 9
            SSK+NK I+
Sbjct: 1193 SSKSNKPII 1201
>XP_005263453
          Length = 1613

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SSKANKEIL 9
            SSK+NK I+
Sbjct: 1482 SSKSNKPII 1490
>NP_004295
          Length = 1620

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SKANKEIL 9
            SK+N+E+L
Sbjct: 1332 SKSNQEVL 1339
>NP_071942
          Length = 173

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  SKANKEIL 9
          +K+NKEI+
Sbjct: 92 TKSNKEIM 99
>NP_055625
          Length = 3117

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SSKANKEIL 9
            SSK NK+++
Sbjct: 2662 SSKENKDLI 2670
>NP_055836
          Length = 2723

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    SSKANKE 7
            SSKAN+E
Sbjct: 2303 SSKANQE 2309
>NP_055045
          Length = 627

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  SKANKE 7
          SKANKE
Sbjct: 48 SKANKE 53
>NP_001247431
          Length = 589

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  SKANKE 7
          SKANKE
Sbjct: 48 SKANKE 53
>XP_005248755
          Length = 2465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    SSKANKE 7
            SSKAN+E
Sbjct: 2045 SSKANQE 2051
>XP_005248754
          Length = 2583

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    SSKANKE 7
            SSKAN+E
Sbjct: 2163 SSKANQE 2169
>XP_006711734
          Length = 3136

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SSKANKEIL 9
            SSK NK+++
Sbjct: 2681 SSKENKDLI 2689
>XP_006711733
          Length = 3137

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SSKANKEIL 9
            SSK NK+++
Sbjct: 2682 SSKENKDLI 2690
>XP_006711732
          Length = 3154

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SSKANKEIL 9
            SSK NK+++
Sbjct: 2699 SSKENKDLI 2707
>XP_006711731
          Length = 3157

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SSKANKEIL 9
            SSK NK+++
Sbjct: 2702 SSKENKDLI 2710
>XP_006711730
          Length = 3157

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SSKANKEIL 9
            SSK NK+++
Sbjct: 2702 SSKENKDLI 2710
>XP_006711729
          Length = 3158

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SSKANKEIL 9
            SSK NK+++
Sbjct: 2703 SSKENKDLI 2711
>XP_005245695
          Length = 3158

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SSKANKEIL 9
            SSK NK+++
Sbjct: 2703 SSKENKDLI 2711
>XP_006711403
          Length = 961

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KANKEIL 9
           KAN+EIL
Sbjct: 374 KANQEIL 380
>NP_653300
          Length = 1029

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KANKEIL 9
           KAN+EIL
Sbjct: 442 KANQEIL 448
>NP_000867
          Length = 2491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KANKEI 8
           KANKE+
Sbjct: 167 KANKEV 172
>NP_002894
          Length = 200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ANKEIL 9
           ANKEIL
Sbjct: 178 ANKEIL 183
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.289    0.108    0.236 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 402,669
Number of extensions: 738
Number of successful extensions: 55
Number of sequences better than 10000.0: 55
Number of HSP's gapped: 55
Number of HSP's successfully gapped: 55
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)

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