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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              17    2528
NP_002909                                                              17    3301
XP_006722894                                                           17    3301
XP_006722893                                                           17    3301
XP_006722892                                                           17    3301
NP_689594                                                              16    5631
XP_005261689                                                           16    5631
XP_006724326                                                           16    5631
XP_006712319                                                           16    5631
NP_001186073                                                           16    5631
NP_079104                                                              16    7355
NP_671710                                                              16    7355
NP_079497                                                              16    7355
XP_005251638                                                           16    7355
NP_001025178                                                           15    9605
NP_001119                                                              15    9605
NP_001012992                                                           15    9605
NP_001258811                                                           15    9605
XP_006715078                                                           15    9605

>NP_115803
          Length = 1156

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSISAQLQQ 9
           SSISAQ QQ
Sbjct: 622 SSISAQQQQ 630




 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSISAQLQQ 9
           SSISAQ QQ
Sbjct: 634 SSISAQQQQ 642



>NP_002909
          Length = 979

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SSISAQLQQ 9
           S ISAQ+QQ
Sbjct: 564 SDISAQVQQ 572



>XP_006722894
          Length = 870

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SSISAQLQQ 9
           S ISAQ+QQ
Sbjct: 455 SDISAQVQQ 463



>XP_006722893
          Length = 870

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SSISAQLQQ 9
           S ISAQ+QQ
Sbjct: 455 SDISAQVQQ 463



>XP_006722892
          Length = 979

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SSISAQLQQ 9
           S ISAQ+QQ
Sbjct: 564 SDISAQVQQ 572



>NP_689594
          Length = 3549

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SSISAQLQQ 9
           SS+SA +QQ
Sbjct: 533 SSVSADVQQ 541



>XP_005261689
          Length = 677

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  SSISAQLQ 8
          +S+SAQLQ
Sbjct: 76 ASVSAQLQ 83



>XP_006724326
          Length = 717

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  SSISAQLQ 8
          +S+SAQLQ
Sbjct: 76 ASVSAQLQ 83



>XP_006712319
          Length = 3308

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SSISAQLQQ 9
           SS+SA +QQ
Sbjct: 292 SSVSADVQQ 300



>NP_001186073
          Length = 3327

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SSISAQLQQ 9
           SS+SA +QQ
Sbjct: 311 SSVSADVQQ 319



>NP_079104
          Length = 361

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SSISAQLQQ 9
           SS+S  LQQ
Sbjct: 93  SSVSGSLQQ 101



>NP_671710
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SISAQLQQ 9
          SISAQL++
Sbjct: 8  SISAQLEE 15



>NP_079497
          Length = 250

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SISAQLQQ 9
          SISAQL++
Sbjct: 8  SISAQLEE 15



>XP_005251638
          Length = 237

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SSISAQLQQ 9
           SS+S  LQQ
Sbjct: 93  SSVSGSLQQ 101



>NP_001025178
          Length = 825

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SSISAQLQQ 9
           SSI  +LQQ
Sbjct: 556 SSIDVELQQ 564



>NP_001119
          Length = 822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SSISAQLQQ 9
           SSI  +LQQ
Sbjct: 553 SSIDVELQQ 561



>NP_001012992
          Length = 316

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SSISAQLQQ 9
           +SIS +LQQ
Sbjct: 240 NSISPELQQ 248



>NP_001258811
          Length = 193

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SSISAQLQQ 9
           +SIS +LQQ
Sbjct: 117 NSISPELQQ 125



>XP_006715078
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SSISAQLQQ 9
           +SIS +LQQ
Sbjct: 199 NSISPELQQ 207



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.293    0.100    0.204 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 299,226
Number of extensions: 349
Number of successful extensions: 20
Number of sequences better than 10000.0: 19
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)