Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              18    1935
XP_006716142                                                           18    1935
XP_005250088                                                           18    1935
XP_006716141                                                           18    1935
XP_005250085                                                           18    1935
XP_006716140                                                           18    1935
XP_005250084                                                           18    1935
XP_005250083                                                           18    1935
XP_005250082                                                           18    1935
NP_073747                                                              16    5631
NP_004710                                                              16    5631
XP_006719755                                                           16    5631
XP_005269287                                                           16    5631
XP_005271187                                                           16    5631
NP_056080                                                              16    7355
NP_001165980                                                           16    7355
NP_001244030                                                           16    7355
NP_001165988                                                           16    7355
NP_056070                                                              16    7355
NP_115749                                                              16    7355
NP_001243478                                                           16    7355
XP_005253860                                                           16    7355
XP_006718094                                                           16    7355
XP_006718093                                                           16    7355
XP_006718092                                                           16    7355
XP_006718091                                                           16    7355
XP_006718090                                                           16    7355
XP_005249023                                                           16    7355
XP_005249022                                                           16    7355
XP_005246453                                                           16    7355
XP_006712435                                                           16    7355
>NP_733751
          Length = 4911

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSADKSKAD 9
           SSAD SKAD
Sbjct: 772 SSADISKAD 780
>XP_006716142
          Length = 4927

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSADKSKAD 9
           SSAD SKAD
Sbjct: 773 SSADISKAD 781
>XP_005250088
          Length = 4928

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSADKSKAD 9
           SSAD SKAD
Sbjct: 773 SSADISKAD 781
>XP_006716141
          Length = 4959

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSADKSKAD 9
           SSAD SKAD
Sbjct: 773 SSADISKAD 781
>XP_005250085
          Length = 4979

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSADKSKAD 9
           SSAD SKAD
Sbjct: 773 SSADISKAD 781
>XP_006716140
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSADKSKAD 9
           SSAD SKAD
Sbjct: 773 SSADISKAD 781
>XP_005250084
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSADKSKAD 9
           SSAD SKAD
Sbjct: 773 SSADISKAD 781
>XP_005250083
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSADKSKAD 9
           SSAD SKAD
Sbjct: 772 SSADISKAD 780
>XP_005250082
          Length = 4983

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSADKSKAD 9
           SSAD SKAD
Sbjct: 773 SSADISKAD 781
>NP_073747
          Length = 532

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SADKSKAD 9
           SAD+S+AD
Sbjct: 360 SADQSQAD 367
>NP_004710
          Length = 1463

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    SSADKSKAD 9
            +SAD SK D
Sbjct: 1366 ASADSSKTD 1374
>XP_006719755
          Length = 1418

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    SSADKSKAD 9
            +SAD SK D
Sbjct: 1321 ASADSSKTD 1329
>XP_005269287
          Length = 1527

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    SSADKSKAD 9
            +SAD SK D
Sbjct: 1430 ASADSSKTD 1438
>XP_005271187
          Length = 406

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SADKSKAD 9
           SAD+S+AD
Sbjct: 234 SADQSQAD 241
>NP_056080
          Length = 733

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SADKSKA 8
           +ADKSKA
Sbjct: 719 NADKSKA 725
>NP_001165980
          Length = 733

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SADKSKA 8
           +ADKSKA
Sbjct: 719 NADKSKA 725
>NP_001244030
          Length = 256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   SSADKSKAD 9
           S ADK K D
Sbjct: 112 SKADKPKVD 120
>NP_001165988
          Length = 733

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SADKSKA 8
           +ADKSKA
Sbjct: 719 NADKSKA 725
>NP_056070
          Length = 1755

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    SSADKSKAD 9
            SS DK KA+
Sbjct: 1022 SSRDKDKAE 1030
>NP_115749
          Length = 256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   SSADKSKAD 9
           S ADK K D
Sbjct: 112 SKADKPKVD 120
>NP_001243478
          Length = 256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   SSADKSKAD 9
           S ADK K D
Sbjct: 112 SKADKPKVD 120
>XP_005253860
          Length = 267

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SSADKSKAD 9
           +S+DKS+ D
Sbjct: 243 NSSDKSRGD 251
>XP_006718094
          Length = 190

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  SSADKSKAD 9
          S ADK K D
Sbjct: 46 SKADKPKVD 54
>XP_006718093
          Length = 191

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  SSADKSKAD 9
          S ADK K D
Sbjct: 47 SKADKPKVD 55
>XP_006718092
          Length = 191

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  SSADKSKAD 9
          S ADK K D
Sbjct: 47 SKADKPKVD 55
>XP_006718091
          Length = 236

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   SSADKSKAD 9
           S ADK K D
Sbjct: 112 SKADKPKVD 120
>XP_006718090
          Length = 255

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   SSADKSKAD 9
           S ADK K D
Sbjct: 111 SKADKPKVD 119
>XP_005249023
          Length = 1052

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SSADKSKAD 9
           SS DK KA+
Sbjct: 319 SSRDKDKAE 327
>XP_005249022
          Length = 1755

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    SSADKSKAD 9
            SS DK KA+
Sbjct: 1022 SSRDKDKAE 1030
>XP_005246453
          Length = 675

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SADKSKA 8
           +ADKSKA
Sbjct: 661 NADKSKA 667
>XP_006712435
          Length = 733

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SADKSKA 8
           +ADKSKA
Sbjct: 719 NADKSKA 725
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.274   0.0915    0.202 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 418,496
Number of extensions: 550
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.5 bits)
S2: 28 (15.4 bits)

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