Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              19     869
NP_009297                                                              19     869
NP_689594                                                              16    5631
NP_001186074                                                           16    5631
NP_001186072                                                           16    5631
NP_001073278                                                           16    5631
XP_006712319                                                           16    5631
NP_001186073                                                           16    5631
NP_055054                                                              16    7355
NP_031397                                                              15    9605
XP_005272271                                                           15    9605
XP_006717354                                                           15    9605
>NP_005148
          Length = 1130

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SRSSEQMAS 9
            SR+SEQMAS
Sbjct: 1044 SRNSEQMAS 1052
>NP_009297
          Length = 1149

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SRSSEQMAS 9
            SR+SEQMAS
Sbjct: 1063 SRNSEQMAS 1071
>NP_689594
          Length = 3549

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SRSSEQMA 8
           SRSS++MA
Sbjct: 297 SRSSQEMA 304
>NP_001186074
          Length = 716

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  SRSSEQMA 8
          SRSS++MA
Sbjct: 75 SRSSQEMA 82
>NP_001186072
          Length = 971

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SRSSEQMA 8
           SRSS++MA
Sbjct: 330 SRSSQEMA 337
>NP_001073278
          Length = 938

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SRSSEQMA 8
           SRSS++MA
Sbjct: 297 SRSSQEMA 304
>XP_006712319
          Length = 3308

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  SRSSEQMA 8
          SRSS++MA
Sbjct: 56 SRSSQEMA 63
>NP_001186073
          Length = 3327

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  SRSSEQMA 8
          SRSS++MA
Sbjct: 75 SRSSQEMA 82
>NP_055054
          Length = 351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RSSEQMA 8
          RSSE+MA
Sbjct: 29 RSSEEMA 35
>NP_031397
          Length = 726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SRSSEQMAS 9
           SRSSE+ +S
Sbjct: 440 SRSSEESSS 448
>XP_005272271
          Length = 725

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SRSSEQMAS 9
           SRSSE+ +S
Sbjct: 440 SRSSEESSS 448
>XP_006717354
          Length = 726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SRSSEQMAS 9
           SRSSE+ +S
Sbjct: 440 SRSSEESSS 448
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.276   0.0834    0.166 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 249,139
Number of extensions: 156
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.7 bits)
S2: 28 (15.4 bits)

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