Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              20     509
NP_671714                                                              20     509
XP_006715890                                                           20     509
XP_005250160                                                           20     509
XP_005250159                                                           20     509
NP_037444                                                              17    4312
NP_001093646                                                           17    4312
XP_005259935                                                           17    4312
XP_005259934                                                           17    4312
XP_005259933                                                           17    4312
XP_005259931                                                           17    4312
XP_005259930                                                           17    4312
NP_055625                                                              16    5631
NP_078824                                                              16    5631
XP_006716441                                                           16    5631
XP_005247816                                                           16    5631
XP_006713806                                                           16    5631
XP_006711734                                                           16    5631
XP_006711733                                                           16    5631
XP_006711732                                                           16    5631
XP_006711731                                                           16    5631
XP_006711730                                                           16    5631
XP_006711729                                                           16    5631
XP_005245695                                                           16    5631
NP_055453                                                              15    9605
NP_001074961                                                           15    9605
NP_872313                                                              15    9605
NP_060827                                                              15    9605
NP_001273608                                                           15    9605
XP_006725014                                                           15    9605
XP_006725013                                                           15    9605
XP_006725012                                                           15    9605
XP_005268732                                                           15    9605
XP_006711118                                                           15    9605
XP_006711117                                                           15    9605
>NP_005742
          Length = 3907

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SRQKESMKA 9
            SRQKE+MKA
Sbjct: 1977 SRQKEAMKA 1985
>NP_671714
          Length = 3899

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SRQKESMKA 9
            SRQKE+MKA
Sbjct: 1977 SRQKEAMKA 1985
>XP_006715890
          Length = 3860

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SRQKESMKA 9
            SRQKE+MKA
Sbjct: 1930 SRQKEAMKA 1938
>XP_005250160
          Length = 3939

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SRQKESMKA 9
            SRQKE+MKA
Sbjct: 2009 SRQKEAMKA 2017
>XP_005250159
          Length = 3952

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SRQKESMKA 9
            SRQKE+MKA
Sbjct: 2009 SRQKEAMKA 2017
>NP_037444
          Length = 719

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   RQKESMKA 9
           RQ+E+MKA
Sbjct: 376 RQEEAMKA 383
>NP_001093646
          Length = 717

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   RQKESMKA 9
           RQ+E+MKA
Sbjct: 376 RQEEAMKA 383
>XP_005259935
          Length = 646

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   RQKESMKA 9
           RQ+E+MKA
Sbjct: 303 RQEEAMKA 310
>XP_005259934
          Length = 646

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   RQKESMKA 9
           RQ+E+MKA
Sbjct: 303 RQEEAMKA 310
>XP_005259933
          Length = 646

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   RQKESMKA 9
           RQ+E+MKA
Sbjct: 303 RQEEAMKA 310
>XP_005259931
          Length = 716

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   RQKESMKA 9
           RQ+E+MKA
Sbjct: 376 RQEEAMKA 383
>XP_005259930
          Length = 718

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   RQKESMKA 9
           RQ+E+MKA
Sbjct: 376 RQEEAMKA 383
>NP_055625
          Length = 3117

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQKESMKA 9
            RQKE +KA
Sbjct: 2078 RQKERLKA 2085
>NP_078824
          Length = 865

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QKESMKA 9
           QKES+KA
Sbjct: 240 QKESLKA 246
>XP_006716441
          Length = 427

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RQKESMK 8
           R+KESMK
Sbjct: 171 RKKESMK 177
>XP_005247816
          Length = 806

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QKESMKA 9
           QKES+KA
Sbjct: 240 QKESLKA 246
>XP_006713806
          Length = 831

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QKESMKA 9
           QKES+KA
Sbjct: 206 QKESLKA 212
>XP_006711734
          Length = 3136

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQKESMKA 9
            RQKE +KA
Sbjct: 2097 RQKERLKA 2104
>XP_006711733
          Length = 3137

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQKESMKA 9
            RQKE +KA
Sbjct: 2098 RQKERLKA 2105
>XP_006711732
          Length = 3154

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQKESMKA 9
            RQKE +KA
Sbjct: 2115 RQKERLKA 2122
>XP_006711731
          Length = 3157

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQKESMKA 9
            RQKE +KA
Sbjct: 2118 RQKERLKA 2125
>XP_006711730
          Length = 3157

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQKESMKA 9
            RQKE +KA
Sbjct: 2118 RQKERLKA 2125
>XP_006711729
          Length = 3158

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQKESMKA 9
            RQKE +KA
Sbjct: 2119 RQKERLKA 2126
>XP_005245695
          Length = 3158

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQKESMKA 9
            RQKE +KA
Sbjct: 2119 RQKERLKA 2126
>NP_055453
          Length = 1416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SRQKESMK 8
           +RQK++MK
Sbjct: 614 TRQKKTMK 621
>NP_001074961
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  SRQKESMKA 9
          +++KE MKA
Sbjct: 81 NQEKEEMKA 89
>NP_872313
          Length = 452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  SRQKESMKA 9
          +++KE MKA
Sbjct: 81 NQEKEEMKA 89
>NP_060827
          Length = 491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SRQKESMKA 9
           S+ KE+MKA
Sbjct: 280 SQGKENMKA 288
>NP_001273608
          Length = 307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SRQKESMKA 9
           S+ KE+MKA
Sbjct: 96  SQGKENMKA 104
>XP_006725014
          Length = 1129

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SRQKESMK 8
           +RQK++MK
Sbjct: 587 TRQKKTMK 594
>XP_006725013
          Length = 1389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SRQKESMK 8
           +RQK++MK
Sbjct: 587 TRQKKTMK 594
>XP_006725012
          Length = 1388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SRQKESMK 8
           +RQK++MK
Sbjct: 586 TRQKKTMK 593
>XP_005268732
          Length = 550

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  SRQKESMKA 9
          +++KE MKA
Sbjct: 81 NQEKEEMKA 89
>XP_006711118
          Length = 1129

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SRQKESMK 8
           +RQK++MK
Sbjct: 587 TRQKKTMK 594
>XP_006711117
          Length = 1413

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SRQKESMK 8
           +RQK++MK
Sbjct: 611 TRQKKTMK 618
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.290   0.0969    0.209 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 484,750
Number of extensions: 733
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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