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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_073713                                                              19     869
NP_001159405                                                           18    1482
NP_003826                                                              18    1482
NP_001075424                                                           18    1482
NP_000923                                                              16    7355
NP_003803                                                              16    7355
NP_996882                                                              16    7355
XP_005274109                                                           16    7355
XP_005246989                                                           16    7355
NP_001171812                                                           16    7355
NP_000544                                                              15    9605
NP_775894                                                              15    9605
NP_001072                                                              15    9605

>NP_073713
          Length = 985

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   SRQAVMSG 8
           SRQAVMSG
Sbjct: 94  SRQAVMSG 101



>NP_001159405
          Length = 484

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SRQAVMSGC 9
           S  A+MSGC
Sbjct: 256 SNDAIMSGC 264



>NP_003826
          Length = 674

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SRQAVMSGC 9
           S  A+MSGC
Sbjct: 259 SNDAIMSGC 267



>NP_001075424
          Length = 671

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SRQAVMSGC 9
           S  A+MSGC
Sbjct: 256 SNDAIMSGC 264



>NP_000923
          Length = 1234

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQAVMSGC 9
           RQA++ GC
Sbjct: 351 RQALLWGC 358



>NP_003803
          Length = 832

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  SRQAVMSGC 9
          SRQ  ++GC
Sbjct: 8  SRQPPLAGC 16



>NP_996882
          Length = 1551

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    RQAVMSGC 9
            RQ++MS C
Sbjct: 1532 RQSIMSVC 1539



>XP_005274109
          Length = 1234

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQAVMSGC 9
           RQA++ GC
Sbjct: 351 RQALLWGC 358



>XP_005246989
          Length = 832

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  SRQAVMSGC 9
          SRQ  ++GC
Sbjct: 8  SRQPPLAGC 16



>NP_001171812
          Length = 1167

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQAVMSGC 9
           RQA++ GC
Sbjct: 284 RQALLWGC 291



>NP_000544
          Length = 1432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    QAVMSGC 9
            QAV +GC
Sbjct: 1293 QAVKAGC 1299



>NP_775894
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  AVMSGC 9
          AVM GC
Sbjct: 14 AVMEGC 19



>NP_001072
          Length = 3623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    SRQAVMSGC 9
            S QAV  GC
Sbjct: 1502 SWQAVTGGC 1510



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.120    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 306,563
Number of extensions: 428
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)