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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_064450                                                              19     869
XP_005273257                                                           19     869
NP_001004752                                                           17    3301
NP_001135918                                                           16    7355
NP_065208                                                              16    7355
NP_945327                                                              16    7355
XP_005273533                                                           16    7355
NP_149420                                                              16    7355
NP_065210                                                              16    7355
NP_065209                                                              16    7355
NP_000028                                                              16    7355
NP_000709                                                              15    9605
NP_001230741                                                           15    9605
NP_001005288                                                           15    9605

>NP_064450
          Length = 1722

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SRIIAMGVL 9
           SRIIAMG+L
Sbjct: 563 SRIIAMGLL 571



>XP_005273257
          Length = 1726

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SRIIAMGVL 9
           SRIIAMG+L
Sbjct: 563 SRIIAMGLL 571



>NP_001004752
          Length = 312

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SRIIAMGVL 9
           SRII MG+L
Sbjct: 141 SRIIQMGLL 149



>NP_001135918
          Length = 1897

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    SRIIAMG 7
            SRIIA+G
Sbjct: 1014 SRIIALG 1020



>NP_065208
          Length = 1856

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SRIIAMG 7
           SRIIA+G
Sbjct: 973 SRIIALG 979



>NP_945327
          Length = 599

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IIAMGVL 9
           ++AMGVL
Sbjct: 431 VVAMGVL 437



>XP_005273533
          Length = 1921

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    SRIIAMG 7
            SRIIA+G
Sbjct: 1014 SRIIALG 1020



>NP_149420
          Length = 312

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SRIIAMGV 8
           +R+IA+GV
Sbjct: 137 TRVIALGV 144



>NP_065210
          Length = 1719

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SRIIAMG 7
           SRIIA+G
Sbjct: 973 SRIIALG 979



>NP_065209
          Length = 1881

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SRIIAMG 7
           SRIIA+G
Sbjct: 973 SRIIALG 979



>NP_000028
          Length = 1880

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SRIIAMG 7
           SRIIA+G
Sbjct: 973 SRIIALG 979



>NP_000709
          Length = 2339

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RIIAMGVL 9
            +IIA GVL
Sbjct: 1521 KIIAFGVL 1528



>NP_001230741
          Length = 2237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RIIAMGVL 9
            +IIA GVL
Sbjct: 1521 KIIAFGVL 1528



>NP_001005288
          Length = 314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SRIIAMGV 8
           +RI+AMG+
Sbjct: 141 NRILAMGL 148



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.339    0.149    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 327,539
Number of extensions: 493
Number of successful extensions: 14
Number of sequences better than 10000.0: 14
Number of HSP's gapped: 14
Number of HSP's successfully gapped: 14
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)