Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245200                                                           22     134
NP_000240                                                              22     134
NP_001161089                                                           22     134
NP_001004056                                                           17    3301
NP_892027                                                              17    3301
NP_599152                                                              17    3301
NP_443166                                                              17    3301
XP_006713943                                                           17    3301
XP_005248019                                                           17    3301
XP_005248018                                                           17    3301
XP_006713942                                                           17    3301
XP_005248014                                                           17    3301
XP_006711207                                                           17    3301
NP_001244073                                                           17    4312
NP_001244074                                                           17    4312
NP_689651                                                              17    4312
NP_085911                                                              17    4312
NP_004390                                                              17    4312
XP_005268810                                                           17    4312
XP_005268809                                                           17    4312
XP_006719115                                                           17    4312
XP_005253390                                                           17    4312
XP_006719114                                                           17    4312
XP_006719113                                                           17    4312
XP_006719112                                                           17    4312
XP_006719111                                                           17    4312
XP_006719110                                                           17    4312
XP_006719109                                                           17    4312
XP_006719108                                                           17    4312
XP_006719107                                                           17    4312
XP_005253388                                                           17    4312
XP_006719106                                                           17    4312
XP_006719105                                                           17    4312
XP_006719104                                                           17    4312
NP_036416                                                              17    4312
NP_001276332                                                           16    5631
NP_001276333                                                           16    5631
NP_001276334                                                           16    5631
NP_071757                                                              16    5631
NP_115901                                                              16    5631
XP_006718008                                                           16    5631
XP_005270098                                                           16    5631
XP_005270092                                                           16    5631
XP_006718007                                                           16    5631
NP_689968                                                              16    5631
NP_001010898                                                           16    7355
XP_006710706                                                           16    7355
NP_116145                                                              15    9605
NP_859422                                                              15    9605
NP_001154992                                                           15    9605
NP_056986                                                              15    9605
NP_006339                                                              15    9605
NP_001094832                                                           15    9605
NP_001269802                                                           15    9605
NP_056503                                                              15    9605
XP_005251019                                                           15    9605
XP_005251017                                                           15    9605
XP_005251016                                                           15    9605
XP_005251014                                                           15    9605
XP_005251013                                                           15    9605
>NP_001245200
          Length = 687

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SREMIEIGC 9
           SRE+IEIGC
Sbjct: 225 SRELIEIGC 233
>NP_000240
          Length = 756

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SREMIEIGC 9
           SRE+IEIGC
Sbjct: 225 SRELIEIGC 233
>NP_001161089
          Length = 658

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SREMIEIGC 9
           SRE+IEIGC
Sbjct: 127 SRELIEIGC 135
>NP_001004056
          Length = 546

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   EMIEIGC 9
           EMIE GC
Sbjct: 483 EMIESGC 489
>NP_892027
          Length = 578

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   EMIEIGC 9
           EMIE GC
Sbjct: 515 EMIESGC 521
>NP_599152
          Length = 887

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SREMIEIGC 9
           ++EMI +GC
Sbjct: 366 NQEMIALGC 374
>NP_443166
          Length = 791

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SREMIEIGC 9
           ++EMI +GC
Sbjct: 366 NQEMIALGC 374
>XP_006713943
          Length = 367

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   EMIEIGC 9
           EMIE GC
Sbjct: 304 EMIESGC 310
>XP_005248019
          Length = 367

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   EMIEIGC 9
           EMIE GC
Sbjct: 304 EMIESGC 310
>XP_005248018
          Length = 384

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   EMIEIGC 9
           EMIE GC
Sbjct: 321 EMIESGC 327
>XP_006713942
          Length = 433

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   EMIEIGC 9
           EMIE GC
Sbjct: 370 EMIESGC 376
>XP_005248014
          Length = 532

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   EMIEIGC 9
           EMIE GC
Sbjct: 469 EMIESGC 475
>XP_006711207
          Length = 791

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SREMIEIGC 9
           ++EMI +GC
Sbjct: 366 NQEMIALGC 374
>NP_001244073
          Length = 970

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 174 SREMLETG 181
>NP_001244074
          Length = 880

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 148 SREMLETG 155
>NP_689651
          Length = 970

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 174 SREMLETG 181
>NP_085911
          Length = 906

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 174 SREMLETG 181
>NP_004390
          Length = 856

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 174 SREMLETG 181
>XP_005268810
          Length = 1005

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SREMIEIGC 9
           S EM+ IGC
Sbjct: 981 SLEMVLIGC 989
>XP_005268809
          Length = 1023

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    SREMIEIGC 9
            S EM+ IGC
Sbjct: 999  SLEMVLIGC 1007
>XP_006719115
          Length = 845

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 210 SREMLETG 217
>XP_005253390
          Length = 942

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 210 SREMLETG 217
>XP_006719114
          Length = 954

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 210 SREMLETG 217
>XP_006719113
          Length = 967

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 210 SREMLETG 217
>XP_006719112
          Length = 969

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 148 SREMLETG 155
>XP_006719111
          Length = 969

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 148 SREMLETG 155
>XP_006719110
          Length = 995

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 174 SREMLETG 181
>XP_006719109
          Length = 995

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 174 SREMLETG 181
>XP_006719108
          Length = 995

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 174 SREMLETG 181
>XP_006719107
          Length = 995

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 174 SREMLETG 181
>XP_005253388
          Length = 1006

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 210 SREMLETG 217
>XP_006719106
          Length = 1018

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 210 SREMLETG 217
>XP_006719105
          Length = 1031

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 210 SREMLETG 217
>XP_006719104
          Length = 1031

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SREMIEIG 8
           SREM+E G
Sbjct: 210 SREMLETG 217
>NP_036416
          Length = 1083

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    SREMIEIGC 9
            S EM+ IGC
Sbjct: 1059 SLEMVLIGC 1067
>NP_001276332
          Length = 987

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   REMIEIGC 9
           RE++E+ C
Sbjct: 696 RELLELSC 703
>NP_001276333
          Length = 871

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   REMIEIGC 9
           RE++E+ C
Sbjct: 580 RELLELSC 587
>NP_001276334
          Length = 1030

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   REMIEIGC 9
           RE++E+ C
Sbjct: 739 RELLELSC 746
>NP_071757
          Length = 1030

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   REMIEIGC 9
           RE++E+ C
Sbjct: 739 RELLELSC 746
>NP_115901
          Length = 769

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   REMIEIGC 9
           RE++EI C
Sbjct: 358 RELLEIKC 365
>XP_006718008
          Length = 666

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   REMIEIGC 9
           RE++E+ C
Sbjct: 375 RELLELSC 382
>XP_005270098
          Length = 829

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   REMIEIGC 9
           RE++E+ C
Sbjct: 538 RELLELSC 545
>XP_005270092
          Length = 872

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   REMIEIGC 9
           RE++E+ C
Sbjct: 581 RELLELSC 588
>XP_006718007
          Length = 1050

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   REMIEIGC 9
           RE++E+ C
Sbjct: 759 RELLELSC 766
>NP_689968
          Length = 248

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SREMIEIG 8
          SRE++E+G
Sbjct: 58 SREVLELG 65
>NP_001010898
          Length = 727

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   REMIEIGC 9
           +EM  +GC
Sbjct: 493 KEMFTVGC 500
>XP_006710706
          Length = 727

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   REMIEIGC 9
           +EM  +GC
Sbjct: 493 KEMFTVGC 500
>NP_116145
          Length = 371

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   SREMIEIGC 9
           +RE+  +GC
Sbjct: 92  TRELAALGC 100
>NP_859422
          Length = 839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  REMIEIGC 9
          RE+++ GC
Sbjct: 57 RELLQDGC 64
>NP_001154992
          Length = 823

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  REMIEIGC 9
          RE+++ GC
Sbjct: 57 RELLQDGC 64
>NP_056986
          Length = 2799

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    SREMIEIG 8
            +RE++E+G
Sbjct: 1977 TRELLELG 1984
>NP_006339
          Length = 860

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  REMIEIGC 9
          RE+++ GC
Sbjct: 57 RELLQDGC 64
>NP_001094832
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   SREMIEIG 8
           S+E++E+G
Sbjct: 221 SKEVVELG 228
>NP_001269802
          Length = 2798

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    SREMIEIG 8
            +RE++E+G
Sbjct: 1977 TRELLELG 1984
>NP_056503
          Length = 1060

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SREMIEI 7
           SREM+E+
Sbjct: 148 SREMLEL 154
>XP_005251019
          Length = 1811

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   SREMIEIG 8
           +RE++E+G
Sbjct: 987 TRELLELG 994
>XP_005251017
          Length = 2792

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    SREMIEIG 8
            +RE++E+G
Sbjct: 1971 TRELLELG 1978
>XP_005251016
          Length = 2795

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    SREMIEIG 8
            +RE++E+G
Sbjct: 1971 TRELLELG 1978
>XP_005251014
          Length = 2800

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    SREMIEIG 8
            +RE++E+G
Sbjct: 1977 TRELLELG 1984
>XP_005251013
          Length = 2801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    SREMIEIG 8
            +RE++E+G
Sbjct: 1977 TRELLELG 1984
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.141    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 437,042
Number of extensions: 951
Number of successful extensions: 60
Number of sequences better than 10000.0: 60
Number of HSP's gapped: 60
Number of HSP's successfully gapped: 60
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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