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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              18    1135
NP_065928                                                              17    3301
XP_005256527                                                           17    3301
NP_004546                                                              16    7355
NP_775186                                                              16    7355
NP_775188                                                              16    7355
NP_001231892                                                           16    7355
NP_001104488                                                           16    7355
NP_660093                                                              16    7355
NP_149107                                                              16    7355
NP_892009                                                              16    7355
XP_005268266                                                           16    7355
XP_006720366                                                           16    7355
XP_005268265                                                           16    7355
XP_006718429                                                           16    7355
XP_006718428                                                           16    7355
XP_006718427                                                           16    7355
NP_663735                                                              15    9605
NP_001177738                                                           15    9605
NP_113611                                                              15    9605
NP_001253981                                                           15    9605
XP_005257983                                                           15    9605
NP_068750                                                              15    9605
NP_663748                                                              15    9605
NP_775259                                                              15    9605
NP_001028748                                                           15    9605

>NP_002430
          Length = 1137

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SQTISGIVN 9
           SQ ISGIVN
Sbjct: 486 SQIISGIVN 494



>NP_065928
          Length = 4427

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QTISGIVN 9
           QT++G+VN
Sbjct: 887 QTLAGVVN 894



>XP_005256527
          Length = 4427

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QTISGIVN 9
           QT++G+VN
Sbjct: 887 QTLAGVVN 894



>NP_004546
          Length = 1068

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TISGIVN 9
           T+SG+VN
Sbjct: 837 TLSGLVN 843



>NP_775186
          Length = 1065

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TISGIVN 9
           T+SG+VN
Sbjct: 837 TLSGLVN 843



>NP_775188
          Length = 1075

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TISGIVN 9
           T+SG+VN
Sbjct: 837 TLSGLVN 843



>NP_001231892
          Length = 2488

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SQTISGIV 8
           SQT+SG V
Sbjct: 658 SQTVSGSV 665



>NP_001104488
          Length = 2365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SQTISGIV 8
           SQT+SG V
Sbjct: 571 SQTVSGSV 578



>NP_660093
          Length = 2429

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SQTISGIV 8
           SQT+SG V
Sbjct: 635 SQTVSGSV 642



>NP_149107
          Length = 979

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SQTISGIVN 9
           +Q +SGI+N
Sbjct: 567 NQCMSGIIN 575



>NP_892009
          Length = 2432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SQTISGIV 8
           SQT+SG V
Sbjct: 635 SQTVSGSV 642



>XP_005268266
          Length = 873

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SQTISGIVN 9
           +Q +SGI+N
Sbjct: 461 NQCMSGIIN 469



>XP_006720366
          Length = 971

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SQTISGIVN 9
           +Q +SGI+N
Sbjct: 579 NQCMSGIIN 587



>XP_005268265
          Length = 991

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SQTISGIVN 9
           +Q +SGI+N
Sbjct: 579 NQCMSGIIN 587



>XP_006718429
          Length = 2416

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SQTISGIV 8
           SQT+SG V
Sbjct: 586 SQTVSGSV 593



>XP_006718428
          Length = 2455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SQTISGIV 8
           SQT+SG V
Sbjct: 658 SQTVSGSV 665



>XP_006718427
          Length = 2465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SQTISGIV 8
           SQT+SG V
Sbjct: 635 SQTVSGSV 642



>NP_663735
          Length = 464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SQTISGIVN 9
           S+ +SGI+N
Sbjct: 118 SRFLSGIIN 126



>NP_001177738
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   SQTISGIV 8
           S TISGIV
Sbjct: 317 SITISGIV 324



>NP_113611
          Length = 464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SQTISGIVN 9
           S+ +SGI+N
Sbjct: 118 SRFLSGIIN 126



>NP_001253981
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QTISGIV 8
           QT+SGI+
Sbjct: 400 QTVSGIL 406



>XP_005257983
          Length = 850

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QTISGIV 8
           QT+SGI+
Sbjct: 400 QTVSGIL 406



>NP_068750
          Length = 703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   SQTISGIV 8
           S TISGIV
Sbjct: 348 SITISGIV 355



>NP_663748
          Length = 591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  QTISGIVN 9
          QT+S +VN
Sbjct: 45 QTVSQVVN 52



>NP_775259
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QTISGIV 8
           QT+SGI+
Sbjct: 400 QTVSGIL 406



>NP_001028748
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QTISGIV 8
           QT+SGI+
Sbjct: 400 QTVSGIL 406



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.122    0.296 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 396,141
Number of extensions: 507
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)