Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              18    1135
XP_005262424                                                           18    1135
NP_001092874                                                           16    7355
NP_001092875                                                           16    7355
NP_001153632                                                           16    7355
NP_001153633                                                           16    7355
NP_932173                                                              16    7355
NP_000326                                                              16    7355
XP_006713346                                                           16    7355
XP_006713345                                                           16    7355
NP_008879                                                              15    9605
NP_001099001                                                           15    9605
NP_065870                                                              15    9605
NP_001156001                                                           15    9605
XP_005248808                                                           15    9605
XP_005248806                                                           15    9605
XP_005248805                                                           15    9605
XP_005248802                                                           15    9605
XP_005248801                                                           15    9605
XP_005248800                                                           15    9605
>NP_722576
          Length = 3080

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SQMVEFSVL 9
            SQMVEF VL
Sbjct: 1858 SQMVEFPVL 1866
>XP_005262424
          Length = 2875

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SQMVEFSVL 9
            SQMVEF VL
Sbjct: 1653 SQMVEFPVL 1661
>NP_001092874
          Length = 2016

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SQMVEFSVL 9
            +Q +E+SVL
Sbjct: 1806 TQFIEYSVL 1814
>NP_001092875
          Length = 1998

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SQMVEFSVL 9
            +Q +E+SVL
Sbjct: 1788 TQFIEYSVL 1796
>NP_001153632
          Length = 1983

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SQMVEFSVL 9
            +Q +E+SVL
Sbjct: 1773 TQFIEYSVL 1781
>NP_001153633
          Length = 1962

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SQMVEFSVL 9
            +Q +E+SVL
Sbjct: 1752 TQFIEYSVL 1760
>NP_932173
          Length = 2016

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SQMVEFSVL 9
            +Q +E+SVL
Sbjct: 1806 TQFIEYSVL 1814
>NP_000326
          Length = 2015

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SQMVEFSVL 9
            +Q +E+SVL
Sbjct: 1805 TQFIEYSVL 1813
>XP_006713346
          Length = 2016

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SQMVEFSVL 9
            +Q +E+SVL
Sbjct: 1806 TQFIEYSVL 1814
>XP_006713345
          Length = 2016

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SQMVEFSVL 9
            +Q +E+SVL
Sbjct: 1806 TQFIEYSVL 1814
>NP_008879
          Length = 471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MVEFSV 8
           MVEFSV
Sbjct: 129 MVEFSV 134
>NP_001099001
          Length = 1319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  MVEFSV 8
          MVEFSV
Sbjct: 7  MVEFSV 12
>NP_065870
          Length = 1302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  MVEFSV 8
          MVEFSV
Sbjct: 7  MVEFSV 12
>NP_001156001
          Length = 1515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  MVEFSV 8
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248808
          Length = 1121

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  MVEFSV 8
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248806
          Length = 1317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  MVEFSV 8
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248805
          Length = 1345

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  MVEFSV 8
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248802
          Length = 1515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  MVEFSV 8
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248801
          Length = 1541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  MVEFSV 8
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248800
          Length = 1541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  MVEFSV 8
          MVEFSV
Sbjct: 7  MVEFSV 12
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.128    0.301 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 320,582
Number of extensions: 357
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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