Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              18    1135
NP_116213                                                              16    5631
XP_005253413                                                           16    5631
XP_005253412                                                           16    5631
XP_005263884                                                           16    5631
XP_006712870                                                           16    5631
NP_001635                                                              16    5631
NP_001231892                                                           16    7355
NP_001104488                                                           16    7355
NP_660093                                                              16    7355
NP_065831                                                              16    7355
NP_892009                                                              16    7355
XP_006718429                                                           16    7355
XP_006718428                                                           16    7355
XP_006718427                                                           16    7355
NP_004855                                                              15    9605
NP_001274745                                                           15    9605
NP_055662                                                              15    9605
NP_002536                                                              15    9605
NP_001012393                                                           15    9605
NP_005796                                                              15    9605
XP_005271636                                                           15    9605
XP_005271635                                                           15    9605
XP_005271632                                                           15    9605
XP_006718909                                                           15    9605
XP_005271631                                                           15    9605
XP_006711752                                                           15    9605
XP_006711751                                                           15    9605
XP_006711750                                                           15    9605
XP_006711749                                                           15    9605
XP_005245729                                                           15    9605
XP_005245727                                                           15    9605
XP_005245726                                                           15    9605
XP_005245725                                                           15    9605
XP_005245724                                                           15    9605
XP_005245715                                                           15    9605
XP_006711748                                                           15    9605
XP_006711747                                                           15    9605
XP_006711746                                                           15    9605
XP_006711745                                                           15    9605
XP_006711744                                                           15    9605
XP_006711743                                                           15    9605
XP_006711742                                                           15    9605
>NP_004110
          Length = 993

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SNTGVSIQV 9
           S TGVSIQV
Sbjct: 633 SKTGVSIQV 641
>NP_116213
          Length = 555

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SNTGVSIQ 8
           SNTG+S Q
Sbjct: 274 SNTGLSTQ 281
>XP_005253413
          Length = 191

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  NTGVSIQV 9
          N+GV+IQ+
Sbjct: 91 NSGVTIQI 98
>XP_005253412
          Length = 236

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   NTGVSIQV 9
           N+GV+IQ+
Sbjct: 136 NSGVTIQI 143
>XP_005263884
          Length = 554

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SNTGVSIQ 8
           SNTG+S Q
Sbjct: 273 SNTGLSTQ 280
>XP_006712870
          Length = 555

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SNTGVSIQ 8
           SNTG+S Q
Sbjct: 274 SNTGLSTQ 281
>NP_001635
          Length = 236

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   NTGVSIQV 9
           N+GV+IQ+
Sbjct: 136 NSGVTIQI 143
>NP_001231892
          Length = 2488

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SNTGVSIQ 8
           S+TGVS++
Sbjct: 715 SSTGVSVE 722
>NP_001104488
          Length = 2365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SNTGVSIQ 8
           S+TGVS++
Sbjct: 628 SSTGVSVE 635
>NP_660093
          Length = 2429

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SNTGVSIQ 8
           S+TGVS++
Sbjct: 692 SSTGVSVE 699
>NP_065831
          Length = 1392

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    NTGVSI 7
            NTGVSI
Sbjct: 1325 NTGVSI 1330
>NP_892009
          Length = 2432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SNTGVSIQ 8
           S+TGVS++
Sbjct: 692 SSTGVSVE 699
>XP_006718429
          Length = 2416

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SNTGVSIQ 8
           S+TGVS++
Sbjct: 643 SSTGVSVE 650
>XP_006718428
          Length = 2455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SNTGVSIQ 8
           S+TGVS++
Sbjct: 715 SSTGVSVE 722
>XP_006718427
          Length = 2465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SNTGVSIQ 8
           S+TGVS++
Sbjct: 692 SSTGVSVE 699
>NP_004855
          Length = 308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SNTGVSIQ 8
           ++TGVS+Q
Sbjct: 288 TDTGVSLQ 295
>NP_001274745
          Length = 1078

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 977 NTGVSV 982
>NP_055662
          Length = 1087

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 966 NTGVSV 971
>NP_002536
          Length = 345

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 231 NTGVSV 236
>NP_001012393
          Length = 338

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 224 NTGVSV 229
>NP_005796
          Length = 310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  SNTGVSIQ 8
          S TGVS++
Sbjct: 78 SGTGVSVE 85
>XP_005271636
          Length = 207

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  NTGVSI 7
          NTGVS+
Sbjct: 93 NTGVSV 98
>XP_005271635
          Length = 245

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 131 NTGVSV 136
>XP_005271632
          Length = 326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 231 NTGVSV 236
>XP_006718909
          Length = 337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 223 NTGVSV 228
>XP_005271631
          Length = 344

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 230 NTGVSV 235
>XP_006711752
          Length = 1085

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 966 NTGVSV 971
>XP_006711751
          Length = 820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 626 NTGVSV 631
>XP_006711750
          Length = 930

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 736 NTGVSV 741
>XP_006711749
          Length = 965

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 771 NTGVSV 776
>XP_005245729
          Length = 1067

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 966 NTGVSV 971
>XP_005245727
          Length = 1079

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 977 NTGVSV 982
>XP_005245726
          Length = 1095

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 977 NTGVSV 982
>XP_005245725
          Length = 1096

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 977 NTGVSV 982
>XP_005245724
          Length = 1098

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 977 NTGVSV 982
>XP_005245715
          Length = 1115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 977 NTGVSV 982
>XP_006711748
          Length = 1160

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 966 NTGVSV 971
>XP_006711747
          Length = 1160

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 966 NTGVSV 971
>XP_006711746
          Length = 1160

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 966 NTGVSV 971
>XP_006711745
          Length = 1170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 977 NTGVSV 982
>XP_006711744
          Length = 1171

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 977 NTGVSV 982
>XP_006711743
          Length = 1171

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 977 NTGVSV 982
>XP_006711742
          Length = 1171

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTGVSI 7
           NTGVS+
Sbjct: 977 NTGVSV 982
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.301    0.117    0.282 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 495,895
Number of extensions: 746
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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