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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     665
XP_005262424                                                           19     665
NP_001072993                                                           18    1935
NP_004551                                                              17    4312
XP_006717184                                                           17    4312
XP_005252065                                                           17    4312
NP_001615                                                              16    5631
XP_005266896                                                           16    5631
NP_001265536                                                           16    7355
NP_001265537                                                           16    7355
NP_001265535                                                           16    7355
NP_001186                                                              16    7355
NP_001155177                                                           16    7355
NP_001073881                                                           15    9605
NP_001157570                                                           15    9605
NP_004376                                                              15    9605
NP_065899                                                              15    9605
XP_005252606                                                           15    9605
XP_006713622                                                           15    9605
XP_005247320                                                           15    9605
XP_005247319                                                           15    9605
XP_005247318                                                           15    9605
XP_005247315                                                           15    9605
XP_005247314                                                           15    9605
XP_005247313                                                           15    9605
XP_005247312                                                           15    9605

>NP_722576
          Length = 3080

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SNRITTAFS 9
            S+RITTAFS
Sbjct: 1482 SDRITTAFS 1490



>XP_005262424
          Length = 2875

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SNRITTAFS 9
            S+RITTAFS
Sbjct: 1277 SDRITTAFS 1285



>NP_001072993
          Length = 432

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SNRITTAFS 9
          SNRI TAF+
Sbjct: 77 SNRIKTAFT 85



>NP_004551
          Length = 943

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NRITTAFS 9
           NRIT AF+
Sbjct: 204 NRITAAFT 211



>XP_006717184
          Length = 803

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  NRITTAFS 9
          NRIT AF+
Sbjct: 64 NRITAAFT 71



>XP_005252065
          Length = 803

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  NRITTAFS 9
          NRIT AF+
Sbjct: 64 NRITAAFT 71



>NP_001615
          Length = 1723

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NRITTAFS 9
           N +TTAFS
Sbjct: 856 NTMTTAFS 863



>XP_005266896
          Length = 2131

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NRITTAFS 9
            N +TTAFS
Sbjct: 1264 NTMTTAFS 1271



>NP_001265536
          Length = 554

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NRITTAF 8
           NR+T+AF
Sbjct: 207 NRLTSAF 213



>NP_001265537
          Length = 526

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NRITTAF 8
           NR+T+AF
Sbjct: 179 NRLTSAF 185



>NP_001265535
          Length = 526

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NRITTAF 8
           NR+T+AF
Sbjct: 179 NRLTSAF 185



>NP_001186
          Length = 665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NRITTAF 8
           NR+T+AF
Sbjct: 318 NRLTSAF 324



>NP_001155177
          Length = 540

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NRITTAF 8
           NR+T+AF
Sbjct: 193 NRLTSAF 199



>NP_001073881
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RITTAFS 9
           RIT AFS
Sbjct: 163 RITNAFS 169



>NP_001157570
          Length = 1642

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    ITTAFS 9
            ITTAFS
Sbjct: 1207 ITTAFS 1212



>NP_004376
          Length = 3396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    ITTAFS 9
            ITTAFS
Sbjct: 1207 ITTAFS 1212



>NP_065899
          Length = 1359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SNRITTAFS 9
            S +IT+AFS
Sbjct: 1002 SPKITSAFS 1010



>XP_005252606
          Length = 1221

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SNRITTAFS 9
           S +IT+AFS
Sbjct: 864 SPKITSAFS 872



>XP_006713622
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RITTAFS 9
           RIT AFS
Sbjct: 163 RITNAFS 169



>XP_005247320
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RITTAFS 9
           RIT AFS
Sbjct: 163 RITNAFS 169



>XP_005247319
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RITTAFS 9
           RIT AFS
Sbjct: 163 RITNAFS 169



>XP_005247318
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RITTAFS 9
           RIT AFS
Sbjct: 163 RITNAFS 169



>XP_005247315
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RITTAFS 9
           RIT AFS
Sbjct: 163 RITNAFS 169



>XP_005247314
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RITTAFS 9
           RIT AFS
Sbjct: 163 RITNAFS 169



>XP_005247313
          Length = 1196

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RITTAFS 9
           RIT AFS
Sbjct: 164 RITNAFS 170



>XP_005247312
          Length = 1231

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RITTAFS 9
           RIT AFS
Sbjct: 199 RITNAFS 205



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.115    0.290 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 284,225
Number of extensions: 317
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)