Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     665
NP_001075109                                                           19     665
NP_008958                                                              17    4312
XP_005247133                                                           17    4312
NP_057006                                                              16    5631
NP_001123237                                                           16    5631
NP_001243779                                                           16    5631
NP_596869                                                              16    5631
NP_056374                                                              16    5631
XP_006724887                                                           16    5631
XP_006722501                                                           16    5631
XP_005258301                                                           16    5631
XP_005258300                                                           16    5631
XP_006712787                                                           16    5631
XP_006712786                                                           16    5631
NP_001254479                                                           16    5631
NP_055828                                                              16    7355
NP_001009999                                                           16    7355
NP_001185902                                                           16    7355
NP_065939                                                              16    7355
NP_001185901                                                           16    7355
XP_006712581                                                           16    7355
XP_005246132                                                           16    7355
XP_005271131                                                           16    7355
XP_006710854                                                           16    7355
XP_006710537                                                           16    7355
XP_006710536                                                           16    7355
XP_006710535                                                           16    7355
XP_005245843                                                           16    7355
NP_001073973                                                           16    7355
NP_000670                                                              15    9605
NP_008832                                                              15    9605
NP_004438                                                              15    9605
NP_001018082                                                           15    9605
NP_001017523                                                           15    9605
NP_060382                                                              15    9605
NP_005133                                                              15    9605
NP_055953                                                              15    9605
NP_665803                                                              15    9605
NP_001158608                                                           15    9605
XP_005254461                                                           15    9605
XP_006720603                                                           15    9605
XP_006720602                                                           15    9605
XP_005268702                                                           15    9605
XP_006719120                                                           15    9605
XP_005253397                                                           15    9605
XP_005253396                                                           15    9605
XP_005273967                                                           15    9605
XP_005265876                                                           15    9605
XP_006714884                                                           15    9605
XP_005265875                                                           15    9605
XP_005263140                                                           15    9605
XP_006711599                                                           15    9605
XP_005245543                                                           15    9605
XP_006711598                                                           15    9605
XP_006711597                                                           15    9605
XP_006711596                                                           15    9605
XP_006711595                                                           15    9605
XP_006711594                                                           15    9605
NP_110391                                                              15    9605
NP_001005168                                                           15    9605
>NP_008835
          Length = 4128

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   SNNKELSI 8
           SNNKELSI
Sbjct: 354 SNNKELSI 361
>NP_001075109
          Length = 4097

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   SNNKELSI 8
           SNNKELSI
Sbjct: 354 SNNKELSI 361
>NP_008958
          Length = 1522

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    NNKELSIG 9
            NNKEL  G
Sbjct: 1495 NNKELGTG 1502
>XP_005247133
          Length = 1517

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    NNKELSIG 9
            NNKEL  G
Sbjct: 1490 NNKELGTG 1497
>NP_057006
          Length = 373

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SNNKEL 6
           SNNKEL
Sbjct: 111 SNNKEL 116
>NP_001123237
          Length = 373

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SNNKEL 6
           SNNKEL
Sbjct: 111 SNNKEL 116
>NP_001243779
          Length = 34350

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    NNKELS 7
            NNKELS
Sbjct: 4701 NNKELS 4706
>NP_596869
          Length = 33423

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    NNKELS 7
            NNKELS
Sbjct: 3774 NNKELS 3779
>NP_056374
          Length = 1596

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SNNKELSIG 9
           +NNK+L +G
Sbjct: 279 NNNKDLLLG 287
>XP_006724887
          Length = 373

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SNNKEL 6
           SNNKEL
Sbjct: 111 SNNKEL 116
>XP_006722501
          Length = 1403

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SNNKELSIG 9
           +NNK+L +G
Sbjct: 279 NNNKDLLLG 287
>XP_005258301
          Length = 1539

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SNNKELSIG 9
           +NNK+L +G
Sbjct: 279 NNNKDLLLG 287
>XP_005258300
          Length = 1596

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SNNKELSIG 9
           +NNK+L +G
Sbjct: 279 NNNKDLLLG 287
>XP_006712787
          Length = 33978

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    NNKELS 7
            NNKELS
Sbjct: 4702 NNKELS 4707
>XP_006712786
          Length = 35484

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    NNKELS 7
            NNKELS
Sbjct: 4702 NNKELS 4707
>NP_001254479
          Length = 35991

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    NNKELS 7
            NNKELS
Sbjct: 5018 NNKELS 5023
>NP_055828
          Length = 852

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NNKELSIG 9
           NNK +S+G
Sbjct: 402 NNKPVSLG 409
>NP_001009999
          Length = 876

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NNKELSIG 9
           NNK +S+G
Sbjct: 426 NNKPVSLG 433
>NP_001185902
          Length = 143

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  NNKELSIG 9
          N+K LS+G
Sbjct: 26 NDKHLSVG 33
>NP_065939
          Length = 1094

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  NNKELSIG 9
          N+K LS+G
Sbjct: 85 NDKHLSVG 92
>NP_001185901
          Length = 1035

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  NNKELSIG 9
          N+K LS+G
Sbjct: 26 NDKHLSVG 33
>XP_006712581
          Length = 1047

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  SNNKELS 7
          +NNKE+S
Sbjct: 64 ANNKEIS 70
>XP_005246132
          Length = 1047

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  SNNKELS 7
          +NNKE+S
Sbjct: 64 ANNKEIS 70
>XP_005271131
          Length = 376

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  NNKELSIG 9
          N+K LS+G
Sbjct: 85 NDKHLSVG 92
>XP_006710854
          Length = 1046

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  NNKELSIG 9
          N+K LS+G
Sbjct: 37 NDKHLSVG 44
>XP_006710537
          Length = 878

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NNKELSIG 9
           NNK +S+G
Sbjct: 422 NNKPVSLG 429
>XP_006710536
          Length = 828

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NNKELSIG 9
           NNK +S+G
Sbjct: 422 NNKPVSLG 429
>XP_006710535
          Length = 858

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NNKELSIG 9
           NNK +S+G
Sbjct: 402 NNKPVSLG 409
>XP_005245843
          Length = 872

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NNKELSIG 9
           NNK +S+G
Sbjct: 422 NNKPVSLG 429
>NP_001073973
          Length = 1052

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  SNNKELS 7
          +NNKE+S
Sbjct: 64 ANNKEIS 70
>NP_000670
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SNNKELSI 8
           SN+KEL++
Sbjct: 246 SNSKELTL 253
>NP_008832
          Length = 2548

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   NNKELSIG 9
           N K LSIG
Sbjct: 527 NTKTLSIG 534
>NP_004438
          Length = 822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NNKELSI 8
           NN ELS+
Sbjct: 546 NNSELSV 552
>NP_001018082
          Length = 1104

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NNKELS 7
           NNKE+S
Sbjct: 918 NNKEMS 923
>NP_001017523
          Length = 641

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NNKELS 7
           NNKE+S
Sbjct: 455 NNKEMS 460
>NP_060382
          Length = 1022

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NNKELSIG 9
           +NK LS+G
Sbjct: 224 SNKPLSVG 231
>NP_005133
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  SNNKELSIG 9
          S+N +L+IG
Sbjct: 82 SDNNDLTIG 90
>NP_055953
          Length = 710

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   NNKELSI 8
           N KELSI
Sbjct: 473 NKKELSI 479
>NP_665803
          Length = 1025

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NNKELS 7
           NNKE+S
Sbjct: 840 NNKEMS 845
>NP_001158608
          Length = 986

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NNKELSIG 9
           +NK LS+G
Sbjct: 224 SNKPLSVG 231
>XP_005254461
          Length = 2600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   NNKELSIG 9
           N K LSIG
Sbjct: 508 NTKTLSIG 515
>XP_006720603
          Length = 2619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   NNKELSIG 9
           N K LSIG
Sbjct: 527 NTKTLSIG 534
>XP_006720602
          Length = 2619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   NNKELSIG 9
           N K LSIG
Sbjct: 527 NTKTLSIG 534
>XP_005268702
          Length = 727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NNKELS 7
           NNKE+S
Sbjct: 541 NNKEMS 546
>XP_006719120
          Length = 822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NNKELSI 8
           NN ELS+
Sbjct: 546 NNSELSV 552
>XP_005253397
          Length = 822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NNKELSI 8
           NN ELS+
Sbjct: 546 NNSELSV 552
>XP_005253396
          Length = 833

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NNKELSI 8
           NN ELS+
Sbjct: 557 NNSELSV 563
>XP_005273967
          Length = 392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  SNNKELSIG 9
          S+N +L+IG
Sbjct: 57 SDNNDLTIG 65
>XP_005265876
          Length = 328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SNNKELSI 8
           SN+KEL++
Sbjct: 246 SNSKELTL 253
>XP_006714884
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SNNKELSI 8
           SN+KEL++
Sbjct: 246 SNSKELTL 253
>XP_005265875
          Length = 366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SNNKELSI 8
           SN+KEL++
Sbjct: 246 SNSKELTL 253
>XP_005263140
          Length = 1034

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NNKELSIG 9
           +NK LS+G
Sbjct: 236 SNKPLSVG 243
>XP_006711599
          Length = 185

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  NNKELSI 8
          +NKELS+
Sbjct: 54 DNKELSV 60
>XP_005245543
          Length = 202

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  NNKELSI 8
          +NKELS+
Sbjct: 54 DNKELSV 60
>XP_006711598
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  NNKELSI 8
          +NKELS+
Sbjct: 54 DNKELSV 60
>XP_006711597
          Length = 319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  NNKELSI 8
          +NKELS+
Sbjct: 54 DNKELSV 60
>XP_006711596
          Length = 413

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  NNKELSI 8
          +NKELS+
Sbjct: 54 DNKELSV 60
>XP_006711595
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  NNKELSI 8
          +NKELS+
Sbjct: 54 DNKELSV 60
>XP_006711594
          Length = 442

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  NNKELSI 8
          +NKELS+
Sbjct: 54 DNKELSV 60
>NP_110391
          Length = 508

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  NNKELSI 8
          +NKELS+
Sbjct: 54 DNKELSV 60
>NP_001005168
          Length = 317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NNKELSIG 9
           N KE+S G
Sbjct: 94  NTKEISFG 101
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.290    0.117    0.285 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 590,150
Number of extensions: 1029
Number of successful extensions: 61
Number of sequences better than 10000.0: 61
Number of HSP's gapped: 61
Number of HSP's successfully gapped: 61
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.8 bits)
S2: 28 (15.4 bits)

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