Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              20     390
NP_009297                                                              20     390
NP_060224                                                              17    3301
XP_006722198                                                           17    3301
XP_005257783                                                           17    3301
XP_005257782                                                           17    3301
XP_006722197                                                           17    3301
XP_006722196                                                           17    3301
XP_005249256                                                           17    3301
XP_006715189                                                           17    3301
XP_006715188                                                           17    3301
NP_115509                                                              17    3301
NP_787954                                                              17    4312
NP_005392                                                              17    4312
NP_059130                                                              17    4312
XP_005254520                                                           17    4312
NP_001275930                                                           16    5631
NP_705837                                                              16    5631
NP_060482                                                              16    5631
XP_006726162                                                           16    5631
XP_006724226                                                           16    5631
XP_006724225                                                           16    5631
XP_006715174                                                           16    5631
NP_000576                                                              16    7355
NP_751915                                                              16    7355
XP_006714497                                                           16    7355
NP_001129396                                                           16    7355
NP_998818                                                              15    9605
NP_000584                                                              15    9605
NP_001136254                                                           15    9605
NP_690864                                                              15    9605
NP_001135870                                                           15    9605
NP_002849                                                              15    9605
NP_004970                                                              15    9605
NP_056147                                                              15    9605
NP_036433                                                              15    9605
NP_001073143                                                           15    9605
NP_001977                                                              15    9605
NP_114130                                                              15    9605
NP_001018114                                                           15    9605
NP_004723                                                              15    9605
XP_005256906                                                           15    9605
XP_005250782                                                           15    9605
XP_005267210                                                           15    9605
XP_005272099                                                           15    9605
XP_005272989                                                           15    9605
XP_005271146                                                           15    9605
XP_006710865                                                           15    9605
XP_005271145                                                           15    9605
XP_005270794                                                           15    9605
NP_001116146                                                           15    9605
NP_112485                                                              15    9605
>NP_005148
          Length = 1130

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   NGALRESG 9
           NGALRESG
Sbjct: 670 NGALRESG 677
>NP_009297
          Length = 1149

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   NGALRESG 9
           NGALRESG
Sbjct: 689 NGALRESG 696
>NP_060224
          Length = 1440

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SNGALRESG 9
           SNG L++SG
Sbjct: 950 SNGELQDSG 958
>XP_006722198
          Length = 996

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NGALRESG 9
           N ALRESG
Sbjct: 705 NEALRESG 712
>XP_005257783
          Length = 999

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NGALRESG 9
           N ALRESG
Sbjct: 720 NEALRESG 727
>XP_005257782
          Length = 1000

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NGALRESG 9
           N ALRESG
Sbjct: 720 NEALRESG 727
>XP_006722197
          Length = 1010

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NGALRESG 9
           N ALRESG
Sbjct: 720 NEALRESG 727
>XP_006722196
          Length = 1011

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NGALRESG 9
           N ALRESG
Sbjct: 720 NEALRESG 727
>XP_005249256
          Length = 1054

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SNGALRESG 9
           SNG L++SG
Sbjct: 564 SNGELQDSG 572
>XP_006715189
          Length = 1240

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SNGALRESG 9
           SNG L++SG
Sbjct: 950 SNGELQDSG 958
>XP_006715188
          Length = 1400

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SNGALRESG 9
           SNG L++SG
Sbjct: 950 SNGELQDSG 958
>NP_115509
          Length = 984

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NGALRESG 9
           N ALRESG
Sbjct: 705 NEALRESG 712
>NP_787954
          Length = 1551

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GALRESG 9
           GAL+ESG
Sbjct: 731 GALKESG 737
>NP_005392
          Length = 1187

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SNGALRE 7
           SNGALR+
Sbjct: 760 SNGALRQ 766
>NP_059130
          Length = 1551

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GALRESG 9
           GAL+ESG
Sbjct: 731 GALKESG 737
>XP_005254520
          Length = 1235

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GALRESG 9
           GAL+ESG
Sbjct: 415 GALKESG 421
>NP_001275930
          Length = 802

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   GALRESG 9
           GALRE G
Sbjct: 699 GALREGG 705
>NP_705837
          Length = 811

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   GALRESG 9
           GALRE G
Sbjct: 708 GALREGG 714
>NP_060482
          Length = 2108

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SNGALRESG 9
           SNGA ++SG
Sbjct: 274 SNGAEKKSG 282
>XP_006726162
          Length = 334

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  GALRESG 9
          GALR+SG
Sbjct: 10 GALRKSG 16
>XP_006724226
          Length = 802

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   GALRESG 9
           GALRE G
Sbjct: 699 GALREGG 705
>XP_006724225
          Length = 802

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   GALRESG 9
           GALRE G
Sbjct: 699 GALREGG 705
>XP_006715174
          Length = 334

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  GALRESG 9
          GALR+SG
Sbjct: 10 GALRKSG 16
>NP_000576
          Length = 162

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SNGALRESG 9
           SNG + ESG
Sbjct: 124 SNGNVTESG 132
>NP_751915
          Length = 135

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SNGALRESG 9
           SNG + ESG
Sbjct: 97  SNGNVTESG 105
>XP_006714497
          Length = 274

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  SNGALRES 8
          SNGA +ES
Sbjct: 6  SNGASKES 13
>NP_001129396
          Length = 1590

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   NGALRES 8
           NG LRES
Sbjct: 496 NGQLRES 502
>NP_998818
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  SNGALRESG 9
          S+GALR  G
Sbjct: 58 SSGALRGGG 66
>NP_000584
          Length = 808

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GALRESG 9
           GA+RE G
Sbjct: 778 GAIREGG 784
>NP_001136254
          Length = 1481

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NGALRESG 9
           +GAL ESG
Sbjct: 174 SGALLESG 181
>NP_690864
          Length = 1125

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NGALRESG 9
           +GAL ESG
Sbjct: 174 SGALLESG 181
>NP_001135870
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NGALRESG 9
           NG LR+ G
Sbjct: 153 NGELRQEG 160
>NP_002849
          Length = 659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   NGALRESG 9
           NG L ESG
Sbjct: 106 NGTLIESG 113
>NP_004970
          Length = 647

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NGALRESG 9
           NG L +SG
Sbjct: 452 NGGLEDSG 459
>NP_056147
          Length = 361

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NGALRES 8
           NGA+RE+
Sbjct: 183 NGAVREN 189
>NP_036433
          Length = 1455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NGALRESG 9
           +GAL ESG
Sbjct: 169 SGALLESG 176
>NP_001073143
          Length = 484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  NGALRES 8
          NG+LRE+
Sbjct: 25 NGSLREA 31
>NP_001977
          Length = 484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  NGALRES 8
          NG+LRE+
Sbjct: 25 NGSLREA 31
>NP_114130
          Length = 560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GALRESG 9
           G+LR+SG
Sbjct: 414 GSLRDSG 420
>NP_001018114
          Length = 248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NGALRESG 9
           NG LR+ G
Sbjct: 153 NGELRQEG 160
>NP_004723
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  GALRES 8
          GALRES
Sbjct: 66 GALRES 71
>XP_005256906
          Length = 375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  GALRES 8
          GALRES
Sbjct: 66 GALRES 71
>XP_005250782
          Length = 1441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NGALRESG 9
           +GAL ESG
Sbjct: 169 SGALLESG 176
>XP_005267210
          Length = 464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GALRESG 9
           G+LR+SG
Sbjct: 318 GSLRDSG 324
>XP_005272099
          Length = 975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NGALRES 8
           NG +RES
Sbjct: 576 NGVIRES 582
>XP_005272989
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  SNGALRESG 9
          S+GALR  G
Sbjct: 58 SSGALRGGG 66
>XP_005271146
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  NGALRESG 9
          NG L ESG
Sbjct: 75 NGTLIESG 82
>XP_006710865
          Length = 644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  NGALRESG 9
          NG L ESG
Sbjct: 91 NGTLIESG 98
>XP_005271145
          Length = 659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   NGALRESG 9
           NG L ESG
Sbjct: 106 NGTLIESG 113
>XP_005270794
          Length = 1125

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NGALRESG 9
           +GAL ESG
Sbjct: 174 SGALLESG 181
>NP_001116146
          Length = 236

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   NGALRESG 9
           NG L ESG
Sbjct: 106 NGTLIESG 113
>NP_112485
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NGALRESG 9
           NG LR+ G
Sbjct: 153 NGELRQEG 160
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.295    0.119    0.306 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 665,565
Number of extensions: 1575
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)

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