Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001243398                                                           21     175
NP_036462                                                              21     175
NP_001243397                                                           21     175
XP_005269721                                                           21     175
NP_073150                                                              20     509
NP_001092882                                                           17    3301
NP_006757                                                              17    3301
NP_001092883                                                           17    3301
XP_005273706                                                           17    3301
NP_942133                                                              17    4312
NP_942131                                                              17    4312
NP_942134                                                              17    4312
NP_942135                                                              17    4312
NP_942136                                                              17    4312
XP_006725385                                                           17    4312
XP_006725384                                                           17    4312
XP_006725383                                                           17    4312
XP_005257325                                                           17    4312
XP_005257324                                                           17    4312
XP_005257323                                                           17    4312
XP_006721916                                                           17    4312
NP_055692                                                              16    5631
XP_005257947                                                           16    5631
XP_005257946                                                           16    5631
XP_005257945                                                           16    5631
XP_006722277                                                           16    5631
NP_001277182                                                           16    7355
NP_001005463                                                           16    7355
NP_001010986                                                           16    7355
NP_775965                                                              16    7355
XP_006724816                                                           16    7355
XP_006724815                                                           16    7355
XP_006724814                                                           16    7355
XP_005262462                                                           16    7355
XP_006717806                                                           16    7355
XP_005252726                                                           16    7355
XP_006717805                                                           16    7355
XP_006717804                                                           16    7355
XP_005252725                                                           16    7355
XP_005252724                                                           16    7355
XP_006717803                                                           16    7355
XP_006717802                                                           16    7355
NP_000123                                                              15    9605
NP_079291                                                              15    9605
XP_006717810                                                           15    9605
XP_006717809                                                           15    9605
XP_005269730                                                           15    9605
XP_005269729                                                           15    9605
>NP_001243398
          Length = 1781

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    NAMNGYSM 9
            NAMNGYSM
Sbjct: 1698 NAMNGYSM 1705
>NP_036462
          Length = 2073

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    NAMNGYSM 9
            NAMNGYSM
Sbjct: 1990 NAMNGYSM 1997
>NP_001243397
          Length = 1890

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    NAMNGYSM 9
            NAMNGYSM
Sbjct: 1807 NAMNGYSM 1814
>XP_005269721
          Length = 2073

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    NAMNGYSM 9
            NAMNGYSM
Sbjct: 1990 NAMNGYSM 1997
>NP_073150
          Length = 575

 Score = 19.6 bits (39), Expect =   509
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   SNAMNGYS 8
           SN+MNGYS
Sbjct: 476 SNSMNGYS 483
>NP_001092882
          Length = 2004

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    NAMNGYSM 9
            NAMN Y M
Sbjct: 1920 NAMNSYRM 1927
>NP_006757
          Length = 2004

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    NAMNGYSM 9
            NAMN Y M
Sbjct: 1920 NAMNSYRM 1927
>NP_001092883
          Length = 2004

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    NAMNGYSM 9
            NAMN Y M
Sbjct: 1920 NAMNSYRM 1927
>XP_005273706
          Length = 1997

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    NAMNGYSM 9
            NAMN Y M
Sbjct: 1913 NAMNSYRM 1920
>NP_942133
          Length = 2346

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NAMNGYSM 9
           N MNGY +
Sbjct: 862 NVMNGYCL 869
>NP_942131
          Length = 2383

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NAMNGYSM 9
           N MNGY +
Sbjct: 899 NVMNGYCL 906
>NP_942134
          Length = 2288

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NAMNGYSM 9
           N MNGY +
Sbjct: 804 NVMNGYCL 811
>NP_942135
          Length = 2268

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NAMNGYSM 9
           N MNGY +
Sbjct: 784 NVMNGYCL 791
>NP_942136
          Length = 2346

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NAMNGYSM 9
           N MNGY +
Sbjct: 862 NVMNGYCL 869
>XP_006725385
          Length = 2038

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NAMNGYSM 9
           N MNGY +
Sbjct: 554 NVMNGYCL 561
>XP_006725384
          Length = 2268

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NAMNGYSM 9
           N MNGY +
Sbjct: 784 NVMNGYCL 791
>XP_006725383
          Length = 2338

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NAMNGYSM 9
           N MNGY +
Sbjct: 862 NVMNGYCL 869
>XP_005257325
          Length = 2038

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NAMNGYSM 9
           N MNGY +
Sbjct: 554 NVMNGYCL 561
>XP_005257324
          Length = 2268

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NAMNGYSM 9
           N MNGY +
Sbjct: 784 NVMNGYCL 791
>XP_005257323
          Length = 2268

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NAMNGYSM 9
           N MNGY +
Sbjct: 784 NVMNGYCL 791
>XP_006721916
          Length = 2375

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NAMNGYSM 9
           N MNGY +
Sbjct: 899 NVMNGYCL 906
>NP_055692
          Length = 1942

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SNAMNGY 7
           SNA++GY
Sbjct: 147 SNALSGY 153
>XP_005257947
          Length = 1935

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SNAMNGY 7
           SNA++GY
Sbjct: 147 SNALSGY 153
>XP_005257946
          Length = 1943

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SNAMNGY 7
           SNA++GY
Sbjct: 147 SNALSGY 153
>XP_005257945
          Length = 1969

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SNAMNGY 7
           SNA++GY
Sbjct: 147 SNALSGY 153
>XP_006722277
          Length = 1970

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SNAMNGY 7
           SNA++GY
Sbjct: 147 SNALSGY 153
>NP_001277182
          Length = 353

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NAMNGYS 8
           +A+NGYS
Sbjct: 262 SAVNGYS 268
>NP_001005463
          Length = 551

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SNAMNGY 7
           S +MNGY
Sbjct: 478 STSMNGY 484
>NP_001010986
          Length = 1119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NAMNGY 7
           NAM+GY
Sbjct: 591 NAMDGY 596
>NP_775965
          Length = 1132

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NAMNGY 7
           NAM+GY
Sbjct: 591 NAMDGY 596
>XP_006724816
          Length = 1105

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NAMNGY 7
           NAM+GY
Sbjct: 588 NAMDGY 593
>XP_006724815
          Length = 1105

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NAMNGY 7
           NAM+GY
Sbjct: 588 NAMDGY 593
>XP_006724814
          Length = 1116

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NAMNGY 7
           NAM+GY
Sbjct: 588 NAMDGY 593
>XP_005262462
          Length = 1129

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NAMNGY 7
           NAM+GY
Sbjct: 588 NAMDGY 593
>XP_006717806
          Length = 560

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SNAMNGY 7
           S +MNGY
Sbjct: 487 STSMNGY 493
>XP_005252726
          Length = 587

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SNAMNGY 7
           S +MNGY
Sbjct: 478 STSMNGY 484
>XP_006717805
          Length = 593

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SNAMNGY 7
           S +MNGY
Sbjct: 487 STSMNGY 493
>XP_006717804
          Length = 596

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SNAMNGY 7
           S +MNGY
Sbjct: 487 STSMNGY 493
>XP_005252725
          Length = 596

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SNAMNGY 7
           S +MNGY
Sbjct: 487 STSMNGY 493
>XP_005252724
          Length = 620

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SNAMNGY 7
           S +MNGY
Sbjct: 478 STSMNGY 484
>XP_006717803
          Length = 628

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SNAMNGY 7
           S +MNGY
Sbjct: 487 STSMNGY 493
>XP_006717802
          Length = 629

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SNAMNGY 7
           S +MNGY
Sbjct: 487 STSMNGY 493
>NP_000123
          Length = 2351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    NAMNGYSM 9
            +A+NGY M
Sbjct: 1938 HAINGYIM 1945
>NP_079291
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   AMNGYS 8
           A+NGYS
Sbjct: 262 AVNGYS 267
>XP_006717810
          Length = 691

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   AMNGYS 8
           A+NGYS
Sbjct: 278 AVNGYS 283
>XP_006717809
          Length = 691

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   AMNGYS 8
           A+NGYS
Sbjct: 278 AVNGYS 283
>XP_005269730
          Length = 691

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   AMNGYS 8
           A+NGYS
Sbjct: 278 AVNGYS 283
>XP_005269729
          Length = 692

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   AMNGYS 8
           A+NGYS
Sbjct: 279 AVNGYS 284
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.111    0.299 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 504,172
Number of extensions: 701
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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