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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_071890                                                              17    4312
XP_006715988                                                           17    4312
XP_006715987                                                           17    4312
XP_005250031                                                           17    4312
XP_005250029                                                           17    4312
XP_005250028                                                           17    4312
XP_005250027                                                           17    4312
NP_000258                                                              16    7355
NP_001035957                                                           16    7355
NP_056501                                                              16    7355
XP_006721991                                                           16    7355
XP_006721990                                                           16    7355
XP_006721989                                                           16    7355
XP_005258041                                                           16    7355
XP_006721988                                                           16    7355
XP_006721987                                                           16    7355
XP_006721986                                                           16    7355
XP_005258040                                                           16    7355
XP_006721985                                                           16    7355
NP_001093900                                                           15    9605
NP_004715                                                              15    9605
XP_006719006                                                           15    9605
XP_006714348                                                           15    9605
XP_006714347                                                           15    9605
NP_001093896                                                           15    9605
NP_001093898                                                           15    9605
NP_066982                                                              15    9605
NP_001093897                                                           15    9605
NP_001093899                                                           15    9605

>NP_071890
          Length = 243

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SMQTLFEAT 9
           S+ TLF+AT
Sbjct: 138 SVNTLFQAT 146



>XP_006715988
          Length = 137

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SMQTLFEAT 9
          S+ TLF+AT
Sbjct: 32 SVNTLFQAT 40



>XP_006715987
          Length = 170

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SMQTLFEAT 9
           S+ TLF+AT
Sbjct: 138 SVNTLFQAT 146



>XP_005250031
          Length = 188

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SMQTLFEAT 9
          S+ TLF+AT
Sbjct: 83 SVNTLFQAT 91



>XP_005250029
          Length = 208

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SMQTLFEAT 9
           S+ TLF+AT
Sbjct: 138 SVNTLFQAT 146



>XP_005250028
          Length = 243

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SMQTLFEAT 9
           S+ TLF+AT
Sbjct: 138 SVNTLFQAT 146



>XP_005250027
          Length = 243

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SMQTLFEAT 9
           S+ TLF+AT
Sbjct: 138 SVNTLFQAT 146



>NP_000258
          Length = 2818

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    SMQTLF 6
            SMQTLF
Sbjct: 1270 SMQTLF 1275



>NP_001035957
          Length = 2839

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    SMQTLF 6
            SMQTLF
Sbjct: 1270 SMQTLF 1275



>NP_056501
          Length = 472

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SMQTLFEAT 9
           S+Q LFE T
Sbjct: 152 SLQRLFEPT 160



>XP_006721991
          Length = 1632

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    SMQTLF 6
            SMQTLF
Sbjct: 1280 SMQTLF 1285



>XP_006721990
          Length = 2823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    SMQTLF 6
            SMQTLF
Sbjct: 1280 SMQTLF 1285



>XP_006721989
          Length = 2823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    SMQTLF 6
            SMQTLF
Sbjct: 1280 SMQTLF 1285



>XP_005258041
          Length = 2836

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    SMQTLF 6
            SMQTLF
Sbjct: 1270 SMQTLF 1275



>XP_006721988
          Length = 2846

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    SMQTLF 6
            SMQTLF
Sbjct: 1280 SMQTLF 1285



>XP_006721987
          Length = 2849

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    SMQTLF 6
            SMQTLF
Sbjct: 1280 SMQTLF 1285



>XP_006721986
          Length = 2854

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    SMQTLF 6
            SMQTLF
Sbjct: 1267 SMQTLF 1272



>XP_005258040
          Length = 2857

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    SMQTLF 6
            SMQTLF
Sbjct: 1270 SMQTLF 1275



>XP_006721985
          Length = 2867

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    SMQTLF 6
            SMQTLF
Sbjct: 1280 SMQTLF 1285



>NP_001093900
          Length = 516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SMQTLFE 7
           SMQ LFE
Sbjct: 214 SMQKLFE 220



>NP_004715
          Length = 779

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   QTLFEAT 9
           QTL EAT
Sbjct: 492 QTLLEAT 498



>XP_006719006
          Length = 715

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   QTLFEAT 9
           QTL EAT
Sbjct: 428 QTLLEAT 434



>XP_006714348
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SMQTLFE 7
           SMQ LFE
Sbjct: 176 SMQKLFE 182



>XP_006714347
          Length = 527

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SMQTLFE 7
           SMQ LFE
Sbjct: 225 SMQKLFE 231



>NP_001093896
          Length = 608

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SMQTLFE 7
           SMQ LFE
Sbjct: 306 SMQKLFE 312



>NP_001093898
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SMQTLFE 7
           SMQ LFE
Sbjct: 257 SMQKLFE 263



>NP_066982
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SMQTLFE 7
           SMQ LFE
Sbjct: 306 SMQKLFE 312



>NP_001093897
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SMQTLFE 7
           SMQ LFE
Sbjct: 305 SMQKLFE 311



>NP_001093899
          Length = 558

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SMQTLFE 7
           SMQ LFE
Sbjct: 256 SMQKLFE 262



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.115    0.281 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 304,253
Number of extensions: 348
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)