Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_859075                                                              18    1935
XP_006719432                                                           18    1935
XP_006719431                                                           18    1935
NP_001191675                                                           17    2528
NP_001191674                                                           17    2528
NP_001191673                                                           17    2528
NP_004053                                                              17    2528
XP_005255827                                                           17    2528
XP_005255826                                                           17    2528
XP_005255825                                                           17    2528
NP_001258463                                                           16    7355
NP_001380                                                              16    7355
NP_116098                                                              16    7355
XP_006724038                                                           16    7355
XP_006718108                                                           16    7355
NP_114414                                                              16    7355
NP_116250                                                              15    9605
NP_776188                                                              15    9605
NP_001161302                                                           15    9605
NP_057124                                                              15    9605
XP_006715649                                                           15    9605
XP_006715648                                                           15    9605
XP_005265737                                                           15    9605
XP_005265736                                                           15    9605
>NP_859075
          Length = 283

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SMPLKATIL 9
          S+PLKAT L
Sbjct: 27 SVPLKATFL 35
>XP_006719432
          Length = 283

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SMPLKATIL 9
          S+PLKAT L
Sbjct: 27 SVPLKATFL 35
>XP_006719431
          Length = 283

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SMPLKATIL 9
          S+PLKAT L
Sbjct: 27 SVPLKATFL 35
>NP_001191675
          Length = 732

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SMPLKATI 8
           S+PLKAT+
Sbjct: 725 SVPLKATV 732
>NP_001191674
          Length = 790

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SMPLKATI 8
           S+PLKAT+
Sbjct: 783 SVPLKATV 790
>NP_001191673
          Length = 807

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SMPLKATI 8
           S+PLKAT+
Sbjct: 800 SVPLKATV 807
>NP_004053
          Length = 829

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SMPLKATI 8
           S+PLKAT+
Sbjct: 822 SVPLKATV 829
>XP_005255827
          Length = 651

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SMPLKATI 8
           S+PLKAT+
Sbjct: 644 SVPLKATV 651
>XP_005255826
          Length = 766

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SMPLKATI 8
           S+PLKAT+
Sbjct: 759 SVPLKATV 766
>XP_005255825
          Length = 829

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SMPLKATI 8
           S+PLKAT+
Sbjct: 822 SVPLKATV 829
>NP_001258463
          Length = 1994

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   PLKATI 8
           PLKATI
Sbjct: 313 PLKATI 318
>NP_001380
          Length = 2012

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   PLKATI 8
           PLKATI
Sbjct: 313 PLKATI 318
>NP_116098
          Length = 581

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SMPLKATI 8
           S PLKAT+
Sbjct: 237 SEPLKATL 244
>XP_006724038
          Length = 1907

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   PLKATI 8
           PLKATI
Sbjct: 208 PLKATI 213
>XP_006718108
          Length = 560

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SMPLKATI 8
           S PLKAT+
Sbjct: 237 SEPLKATL 244
>NP_114414
          Length = 585

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   MPLKATI 8
           MP KATI
Sbjct: 295 MPAKATI 301
>NP_116250
          Length = 654

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SMPLKATIL 9
           S P+KA +L
Sbjct: 389 SQPIKADVL 397
>NP_776188
          Length = 333

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   PLKATIL 9
           P KAT+L
Sbjct: 167 PFKATVL 173
>NP_001161302
          Length = 193

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  MPLKATI 8
          MPLKA++
Sbjct: 52 MPLKASL 58
>NP_057124
          Length = 229

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  MPLKATI 8
          MPLKA++
Sbjct: 52 MPLKASL 58
>XP_006715649
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SMPLKATIL 9
           S P+KA +L
Sbjct: 319 SQPIKADVL 327
>XP_006715648
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SMPLKATIL 9
           S P+KA +L
Sbjct: 351 SQPIKADVL 359
>XP_005265737
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   PLKATIL 9
           P KAT+L
Sbjct: 167 PFKATVL 173
>XP_005265736
          Length = 334

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   PLKATIL 9
           P KAT+L
Sbjct: 167 PFKATVL 173
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.130    0.339 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 496,650
Number of extensions: 806
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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