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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_065928                                                              18    1935
XP_005256527                                                           18    1935
NP_955452                                                              17    2528
NP_741996                                                              17    2528
XP_006713543                                                           17    2528
NP_001121364                                                           17    3301
NP_002959                                                              17    3301
XP_006721304                                                           17    3301
NP_116245                                                              17    4312
XP_005255040                                                           17    4312
XP_006720787                                                           17    4312
NP_001153101                                                           16    5631
NP_003636                                                              16    5631
NP_055497                                                              16    7355
NP_006737                                                              16    7355
NP_001032624                                                           16    7355
NP_001258349                                                           16    7355
NP_001032625                                                           16    7355
NP_060245                                                              16    7355
NP_001129631                                                           16    7355
NP_001129630                                                           16    7355
NP_001129629                                                           16    7355
NP_001032629                                                           16    7355
NP_060072                                                              16    7355
XP_005278099                                                           16    7355
XP_005272679                                                           16    7355
XP_006724600                                                           16    7355
XP_005278096                                                           16    7355
XP_005274636                                                           16    7355
XP_006724572                                                           16    7355
XP_006724571                                                           16    7355
XP_005274635                                                           16    7355
XP_006716552                                                           16    7355
XP_006716551                                                           16    7355
XP_006716550                                                           16    7355
NP_001108624                                                           15    9605
NP_620061                                                              15    9605

>NP_065928
          Length = 4427

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    MKKNEISI 9
            MKKN++SI
Sbjct: 1682 MKKNQVSI 1689



>XP_005256527
          Length = 4427

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    MKKNEISI 9
            MKKN++SI
Sbjct: 1682 MKKNQVSI 1689



>NP_955452
          Length = 2590

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    MKKNEISI 9
            +KKNE+S+
Sbjct: 1430 LKKNEVSV 1437



>NP_741996
          Length = 1300

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SMKKNEISI 9
            +MK NEIS+
Sbjct: 1224 AMKNNEISV 1232



>XP_006713543
          Length = 2591

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    MKKNEISI 9
            +KKNE+S+
Sbjct: 1431 LKKNEVSV 1438



>NP_001121364
          Length = 1227

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SMKKNEISI 9
            +MK NEIS+
Sbjct: 1149 AMKANEISV 1157



>NP_002959
          Length = 1324

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SMKKNEISI 9
            +MK NEIS+
Sbjct: 1246 AMKANEISV 1254



>XP_006721304
          Length = 1324

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SMKKNEISI 9
            +MK NEIS+
Sbjct: 1246 AMKANEISV 1254



>NP_116245
          Length = 378

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  SMKKNEI 7
          S+KKNEI
Sbjct: 45 SLKKNEI 51



>XP_005255040
          Length = 178

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  SMKKNEI 7
          S+KKNEI
Sbjct: 45 SLKKNEI 51



>XP_006720787
          Length = 256

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  SMKKNEI 7
          S+KKNEI
Sbjct: 45 SLKKNEI 51



>NP_001153101
          Length = 567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SMKKNEISI 9
           S+KK+++SI
Sbjct: 171 SLKKDDVSI 179



>NP_003636
          Length = 620

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SMKKNEISI 9
           S+KK+++SI
Sbjct: 171 SLKKDDVSI 179



>NP_055497
          Length = 1047

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SMKKNEI 7
           SMKKN I
Sbjct: 617 SMKKNRI 623



>NP_006737
          Length = 302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   MKKNEI 7
           MKKNE+
Sbjct: 95  MKKNEV 100



>NP_001032624
          Length = 208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2 MKKNEI 7
         MKKNE+
Sbjct: 1 MKKNEV 6



>NP_001258349
          Length = 273

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MKKNEISI 9
           +KK+EIS+
Sbjct: 243 LKKDEISV 250



>NP_001032625
          Length = 208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2 MKKNEI 7
         MKKNE+
Sbjct: 1 MKKNEV 6



>NP_060245
          Length = 380

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MKKNEISI 9
           +KK+EIS+
Sbjct: 350 LKKDEISV 357



>NP_001129631
          Length = 349

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SMKKNEI 7
           SMKKN++
Sbjct: 334 SMKKNKV 340



>NP_001129630
          Length = 566

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SMKKNEI 7
           SMKKN++
Sbjct: 551 SMKKNKV 557



>NP_001129629
          Length = 566

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SMKKNEI 7
           SMKKN++
Sbjct: 551 SMKKNKV 557



>NP_001032629
          Length = 329

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   MKKNEI 7
           MKKNE+
Sbjct: 122 MKKNEV 127



>NP_060072
          Length = 571

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SMKKNEI 7
           SMKKN++
Sbjct: 556 SMKKNKV 562



>XP_005278099
          Length = 355

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SMKKNEI 7
           SMKKN++
Sbjct: 340 SMKKNKV 346



>XP_005272679
          Length = 567

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SMKKNEI 7
           SMKKN++
Sbjct: 552 SMKKNKV 558



>XP_006724600
          Length = 571

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SMKKNEI 7
           SMKKN++
Sbjct: 556 SMKKNKV 562



>XP_005278096
          Length = 572

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SMKKNEI 7
           SMKKN++
Sbjct: 557 SMKKNKV 563



>XP_005274636
          Length = 301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  MKKNEI 7
          MKKNE+
Sbjct: 94 MKKNEV 99



>XP_006724572
          Length = 301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  MKKNEI 7
          MKKNE+
Sbjct: 94 MKKNEV 99



>XP_006724571
          Length = 329

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   MKKNEI 7
           MKKNE+
Sbjct: 122 MKKNEV 127



>XP_005274635
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   MKKNEI 7
           MKKNE+
Sbjct: 123 MKKNEV 128



>XP_006716552
          Length = 1038

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SMKKNEI 7
           SMKKN I
Sbjct: 617 SMKKNRI 623



>XP_006716551
          Length = 1047

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SMKKNEI 7
           SMKKN I
Sbjct: 617 SMKKNRI 623



>XP_006716550
          Length = 1047

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SMKKNEI 7
           SMKKN I
Sbjct: 617 SMKKNRI 623



>NP_001108624
          Length = 194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MKKNEISI 9
          +KKN +SI
Sbjct: 74 VKKNRVSI 81



>NP_620061
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  MKKNEIS 8
          MKKN+I+
Sbjct: 29 MKKNQIT 35



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.115    0.256 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 444,003
Number of extensions: 485
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)