Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     869
NP_671714                                                              19     869
XP_006715890                                                           19     869
XP_005250160                                                           19     869
XP_005250159                                                           19     869
NP_001138890                                                           17    3301
XP_006724234                                                           17    3301
XP_005261462                                                           17    3301
NP_005207                                                              17    4312
NP_114091                                                              15    9605
NP_073610                                                              15    9605
NP_001020278                                                           15    9605
NP_056655                                                              15    9605
NP_056656                                                              15    9605
NP_001102                                                              15    9605
NP_001180424                                                           15    9605
XP_005256137                                                           15    9605
XP_006720992                                                           15    9605
XP_006720991                                                           15    9605
XP_006720990                                                           15    9605
XP_006720989                                                           15    9605
XP_006711176                                                           15    9605
XP_006711175                                                           15    9605
XP_006711174                                                           15    9605
XP_006711173                                                           15    9605
XP_006711172                                                           15    9605
>NP_005742
          Length = 3907

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMKDRAMEL 9
            SMKDRA EL
Sbjct: 3142 SMKDRATEL 3150
>NP_671714
          Length = 3899

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMKDRAMEL 9
            SMKDRA EL
Sbjct: 3134 SMKDRATEL 3142
>XP_006715890
          Length = 3860

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMKDRAMEL 9
            SMKDRA EL
Sbjct: 3095 SMKDRATEL 3103
>XP_005250160
          Length = 3939

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMKDRAMEL 9
            SMKDRA EL
Sbjct: 3174 SMKDRATEL 3182
>XP_005250159
          Length = 3952

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMKDRAMEL 9
            SMKDRA EL
Sbjct: 3187 SMKDRATEL 3195
>NP_001138890
          Length = 2481

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MKDRAME 8
           MKDRA+E
Sbjct: 990 MKDRALE 996
>XP_006724234
          Length = 2363

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MKDRAME 8
           MKDRA+E
Sbjct: 872 MKDRALE 878
>XP_005261462
          Length = 2473

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MKDRAME 8
           MKDRA+E
Sbjct: 990 MKDRALE 996
>NP_005207
          Length = 456

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  SMKDRAMEL 9
          S+KDR  EL
Sbjct: 66 SLKDRGFEL 74
>NP_114091
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SMKDRAMEL 9
           +MK R MEL
Sbjct: 639 TMKSRYMEL 647
>NP_073610
          Length = 567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   SMKDRAMEL 9
           S+KDR +++
Sbjct: 338 SLKDRVLQM 346
>NP_001020278
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   SMKDRAME 8
           S  DRAME
Sbjct: 680 SCSDRAME 687
>NP_056655
          Length = 1200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   SMKDRAME 8
           S  DRAME
Sbjct: 949 SCSDRAME 956
>NP_056656
          Length = 1181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   SMKDRAME 8
           S  DRAME
Sbjct: 930 SCSDRAME 937
>NP_001102
          Length = 1226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   SMKDRAME 8
           S  DRAME
Sbjct: 975 SCSDRAME 982
>NP_001180424
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   SMKDRAME 8
           S  DRAME
Sbjct: 680 SCSDRAME 687
>XP_005256137
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SMKDRAMEL 9
           +MK R MEL
Sbjct: 639 TMKSRYMEL 647
>XP_006720992
          Length = 434

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   SMKDRAMEL 9
           S+KDR +++
Sbjct: 205 SLKDRVLQM 213
>XP_006720991
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   SMKDRAMEL 9
           S+KDR +++
Sbjct: 229 SLKDRVLQM 237
>XP_006720990
          Length = 489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   SMKDRAMEL 9
           S+KDR +++
Sbjct: 260 SLKDRVLQM 268
>XP_006720989
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   SMKDRAMEL 9
           S+KDR +++
Sbjct: 280 SLKDRVLQM 288
>XP_006711176
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   SMKDRAME 8
           S  DRAME
Sbjct: 680 SCSDRAME 687
>XP_006711175
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   SMKDRAME 8
           S  DRAME
Sbjct: 680 SCSDRAME 687
>XP_006711174
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   SMKDRAME 8
           S  DRAME
Sbjct: 680 SCSDRAME 687
>XP_006711173
          Length = 1210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   SMKDRAME 8
           S  DRAME
Sbjct: 959 SCSDRAME 966
>XP_006711172
          Length = 1236

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   SMKDRAME 8
           S  DRAME
Sbjct: 985 SCSDRAME 992
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.121    0.292 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 406,513
Number of extensions: 688
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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