Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              18    1935
NP_001035957                                                           18    1935
XP_006721991                                                           18    1935
XP_006721990                                                           18    1935
XP_006721989                                                           18    1935
XP_005258041                                                           18    1935
XP_006721988                                                           18    1935
XP_006721987                                                           18    1935
XP_006721986                                                           18    1935
XP_005258040                                                           18    1935
XP_006721985                                                           18    1935
NP_059116                                                              17    4312
NP_064578                                                              17    4312
XP_005267816                                                           17    4312
XP_006718702                                                           17    4312
XP_006718701                                                           17    4312
XP_006718700                                                           17    4312
XP_005274169                                                           17    4312
XP_005274166                                                           17    4312
XP_005274165                                                           17    4312
XP_005274163                                                           17    4312
NP_001273762                                                           16    5631
NP_057013                                                              16    5631
NP_660199                                                              16    5631
NP_660198                                                              16    5631
NP_005246                                                              16    5631
NP_001191759                                                           16    5631
XP_005272330                                                           16    5631
XP_005272329                                                           16    5631
XP_005272327                                                           16    5631
XP_006713937                                                           16    5631
XP_005272326                                                           16    5631
XP_005272325                                                           16    5631
NP_660200                                                              16    5631
>NP_000258
          Length = 2818

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMILQSIAN 9
            S ILQSIAN
Sbjct: 1422 SKILQSIAN 1430
>NP_001035957
          Length = 2839

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMILQSIAN 9
            S ILQSIAN
Sbjct: 1443 SKILQSIAN 1451
>XP_006721991
          Length = 1632

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMILQSIAN 9
            S ILQSIAN
Sbjct: 1453 SKILQSIAN 1461
>XP_006721990
          Length = 2823

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMILQSIAN 9
            S ILQSIAN
Sbjct: 1453 SKILQSIAN 1461
>XP_006721989
          Length = 2823

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMILQSIAN 9
            S ILQSIAN
Sbjct: 1453 SKILQSIAN 1461
>XP_005258041
          Length = 2836

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMILQSIAN 9
            S ILQSIAN
Sbjct: 1422 SKILQSIAN 1430
>XP_006721988
          Length = 2846

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMILQSIAN 9
            S ILQSIAN
Sbjct: 1432 SKILQSIAN 1440
>XP_006721987
          Length = 2849

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMILQSIAN 9
            S ILQSIAN
Sbjct: 1453 SKILQSIAN 1461
>XP_006721986
          Length = 2854

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMILQSIAN 9
            S ILQSIAN
Sbjct: 1440 SKILQSIAN 1448
>XP_005258040
          Length = 2857

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMILQSIAN 9
            S ILQSIAN
Sbjct: 1443 SKILQSIAN 1451
>XP_006721985
          Length = 2867

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMILQSIAN 9
            S ILQSIAN
Sbjct: 1453 SKILQSIAN 1461
>NP_059116
          Length = 781

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MILQSIAN 9
           ++LQS+AN
Sbjct: 713 LVLQSVAN 720
>NP_064578
          Length = 1322

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SMILQSIAN 9
           S I+QSIAN
Sbjct: 242 SNIMQSIAN 250
>XP_005267816
          Length = 773

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MILQSIAN 9
           ++LQS+AN
Sbjct: 705 LVLQSVAN 712
>XP_006718702
          Length = 1151

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SMILQSIAN 9
           S I+QSIAN
Sbjct: 192 SNIMQSIAN 200
>XP_006718701
          Length = 1309

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SMILQSIAN 9
           S I+QSIAN
Sbjct: 243 SNIMQSIAN 251
>XP_006718700
          Length = 1216

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SMILQSIAN 9
           S I+QSIAN
Sbjct: 257 SNIMQSIAN 265
>XP_005274169
          Length = 1272

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SMILQSIAN 9
           S I+QSIAN
Sbjct: 206 SNIMQSIAN 214
>XP_005274166
          Length = 1323

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SMILQSIAN 9
           S I+QSIAN
Sbjct: 257 SNIMQSIAN 265
>XP_005274165
          Length = 1323

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SMILQSIAN 9
           S I+QSIAN
Sbjct: 243 SNIMQSIAN 251
>XP_005274163
          Length = 1337

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SMILQSIAN 9
           S I+QSIAN
Sbjct: 257 SNIMQSIAN 265
>NP_001273762
          Length = 1213

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SMILQSIAN 9
           S ++QS+AN
Sbjct: 296 SKVIQSVAN 304
>NP_057013
          Length = 373

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  MILQSIAN 9
          +ILQSI+N
Sbjct: 32 LILQSISN 39
>NP_660199
          Length = 373

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  MILQSIAN 9
          +ILQSI+N
Sbjct: 32 LILQSISN 39
>NP_660198
          Length = 373

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  MILQSIAN 9
          +ILQSI+N
Sbjct: 32 LILQSISN 39
>NP_005246
          Length = 1311

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SMILQSIAN 9
           S ++QS+AN
Sbjct: 394 SKVIQSVAN 402
>NP_001191759
          Length = 373

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  MILQSIAN 9
          +ILQSI+N
Sbjct: 32 LILQSISN 39
>XP_005272330
          Length = 1140

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SMILQSIAN 9
           S ++QS+AN
Sbjct: 394 SKVIQSVAN 402
>XP_005272329
          Length = 1175

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SMILQSIAN 9
           S ++QS+AN
Sbjct: 258 SKVIQSVAN 266
>XP_005272327
          Length = 1236

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SMILQSIAN 9
           S ++QS+AN
Sbjct: 394 SKVIQSVAN 402
>XP_006713937
          Length = 1249

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SMILQSIAN 9
           S ++QS+AN
Sbjct: 332 SKVIQSVAN 340
>XP_005272326
          Length = 1262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SMILQSIAN 9
           S ++QS+AN
Sbjct: 394 SKVIQSVAN 402
>XP_005272325
          Length = 1300

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SMILQSIAN 9
           S ++QS+AN
Sbjct: 394 SKVIQSVAN 402
>NP_660200
          Length = 373

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  MILQSIAN 9
          +ILQSI+N
Sbjct: 32 LILQSISN 39
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.122    0.275 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 217,088
Number of extensions: 202
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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