Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              17    2528
NP_444254                                                              17    2528
NP_444253                                                              17    2528
NP_444255                                                              17    2528
XP_005247551                                                           17    2528
XP_005247550                                                           17    2528
XP_006713712                                                           17    2528
XP_005247549                                                           17    2528
NP_000171                                                              16    5631
NP_444260                                                              16    7355
NP_444259                                                              16    7355
NP_005493                                                              16    7355
NP_001243624                                                           16    7355
XP_006726338                                                           16    7355
XP_005251835                                                           16    7355
XP_006717058                                                           16    7355
XP_005251833                                                           16    7355
XP_005251831                                                           16    7355
XP_005251830                                                           16    7355
NP_004377                                                              15    9605
NP_001191790                                                           15    9605
NP_001191791                                                           15    9605
NP_001191788                                                           15    9605
NP_005639                                                              15    9605
NP_001191792                                                           15    9605
NP_001191789                                                           15    9605
NP_001191787                                                           15    9605
NP_001191793                                                           15    9605
NP_001191786                                                           15    9605
XP_005259803                                                           15    9605
XP_005259802                                                           15    9605
XP_005251347                                                           15    9605
XP_005270977                                                           15    9605
XP_001718723                                                           15    9605
NP_057575                                                              15    9605
>NP_444256
          Length = 1794

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMAMISGPS 9
            SMAMISG S
Sbjct: 1640 SMAMISGLS 1648
>NP_444254
          Length = 1845

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMAMISGPS 9
            SMAMISG S
Sbjct: 1691 SMAMISGLS 1699
>NP_444253
          Length = 1914

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMAMISGPS 9
            SMAMISG S
Sbjct: 1760 SMAMISGLS 1768
>NP_444255
          Length = 1863

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMAMISGPS 9
            SMAMISG S
Sbjct: 1709 SMAMISGLS 1717
>XP_005247551
          Length = 1855

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMAMISGPS 9
            SMAMISG S
Sbjct: 1701 SMAMISGLS 1709
>XP_005247550
          Length = 1856

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMAMISGPS 9
            SMAMISG S
Sbjct: 1702 SMAMISGLS 1710
>XP_006713712
          Length = 1923

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMAMISGPS 9
            SMAMISG S
Sbjct: 1770 SMAMISGLS 1778
>XP_005247549
          Length = 1924

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SMAMISGPS 9
            SMAMISG S
Sbjct: 1770 SMAMISGLS 1778
>NP_000171
          Length = 1103

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   MAMISGPS 9
           M M+SGP+
Sbjct: 494 MQMVSGPN 501
>NP_444260
          Length = 154

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2 MAMISGPS 9
         MAMISG S
Sbjct: 1 MAMISGLS 8
>NP_444259
          Length = 153

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2 MAMISGPS 9
         MAMISG S
Sbjct: 1 MAMISGLS 8
>NP_005493
          Length = 2261

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    SMAMISGP 8
            +MA+I GP
Sbjct: 2056 AMALIGGP 2063
>NP_001243624
          Length = 1177

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SMAMISGPS 9
           S +M+SGP+
Sbjct: 458 SESMVSGPA 466
>XP_006726338
          Length = 481

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MISGPS 9
           M+SGPS
Sbjct: 417 MVSGPS 422
>XP_005251835
          Length = 2074

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    SMAMISGP 8
            +MA+I GP
Sbjct: 2058 AMALIGGP 2065
>XP_006717058
          Length = 2100

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    SMAMISGP 8
            +MA+I GP
Sbjct: 2058 AMALIGGP 2065
>XP_005251833
          Length = 2203

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    SMAMISGP 8
            +MA+I GP
Sbjct: 1998 AMALIGGP 2005
>XP_005251831
          Length = 2263

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    SMAMISGP 8
            +MA+I GP
Sbjct: 2058 AMALIGGP 2065
>XP_005251830
          Length = 2263

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    SMAMISGP 8
            +MA+I GP
Sbjct: 2058 AMALIGGP 2065
>NP_004377
          Length = 1321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   MAMISGP 8
           MAM+ GP
Sbjct: 633 MAMLRGP 639
>NP_001191790
          Length = 112

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  AMISGP 8
          AM+SGP
Sbjct: 44 AMLSGP 49
>NP_001191791
          Length = 112

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  AMISGP 8
          AM+SGP
Sbjct: 44 AMLSGP 49
>NP_001191788
          Length = 112

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  AMISGP 8
          AM+SGP
Sbjct: 44 AMLSGP 49
>NP_005639
          Length = 112

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  AMISGP 8
          AM+SGP
Sbjct: 44 AMLSGP 49
>NP_001191792
          Length = 96

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  AMISGP 8
          AM+SGP
Sbjct: 28 AMLSGP 33
>NP_001191789
          Length = 112

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  AMISGP 8
          AM+SGP
Sbjct: 44 AMLSGP 49
>NP_001191787
          Length = 112

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  AMISGP 8
          AM+SGP
Sbjct: 44 AMLSGP 49
>NP_001191793
          Length = 96

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  AMISGP 8
          AM+SGP
Sbjct: 28 AMLSGP 33
>NP_001191786
          Length = 112

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  AMISGP 8
          AM+SGP
Sbjct: 44 AMLSGP 49
>XP_005259803
          Length = 1321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   MAMISGP 8
           MAM+ GP
Sbjct: 633 MAMLRGP 639
>XP_005259802
          Length = 1321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   MAMISGP 8
           MAM+ GP
Sbjct: 633 MAMLRGP 639
>XP_005251347
          Length = 112

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  AMISGP 8
          AM+SGP
Sbjct: 44 AMLSGP 49
>XP_005270977
          Length = 483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2 MAMISGPS 9
         MA+ SGP+
Sbjct: 1 MALASGPA 8
>XP_001718723
          Length = 1091

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   AMISGPS 9
           AMIS PS
Sbjct: 208 AMISSPS 214
>NP_057575
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2 MAMISGPS 9
         MA+ SGP+
Sbjct: 1 MALASGPA 8
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.114    0.300 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 642,356
Number of extensions: 320
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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