Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              18    1935
NP_001075109                                                           18    1935
NP_001269504                                                           17    4312
NP_001269507                                                           17    4312
NP_001269506                                                           17    4312
NP_954595                                                              17    4312
NP_954673                                                              17    4312
NP_071887                                                              17    4312
NP_001027459                                                           17    4312
NP_068823                                                              17    4312
NP_001244328                                                           17    4312
NP_001244327                                                           17    4312
NP_001244324                                                           17    4312
NP_001244322                                                           17    4312
NP_001269508                                                           17    4312
NP_001269505                                                           17    4312
NP_001244326                                                           17    4312
NP_001244323                                                           17    4312
XP_006726463                                                           17    4312
XP_006712963                                                           17    4312
XP_006710204                                                           17    4312
NP_003341                                                              16    5631
XP_005251357                                                           16    5631
NP_001138775                                                           16    7355
NP_653287                                                              16    7355
NP_006718                                                              16    7355
NP_001008781                                                           16    7355
NP_001273384                                                           16    7355
NP_068764                                                              16    7355
NP_001273385                                                           16    7355
XP_006726250                                                           16    7355
XP_006726210                                                           16    7355
XP_006726112                                                           16    7355
XP_006726013                                                           16    7355
XP_006725925                                                           16    7355
XP_006725825                                                           16    7355
XP_006725698                                                           16    7355
XP_005249342                                                           16    7355
NP_056297                                                              16    7355
NP_443187                                                              16    7355
NP_071410                                                              15    9605
NP_001186562                                                           15    9605
NP_001017403                                                           15    9605
NP_067649                                                              15    9605
NP_001017404                                                           15    9605
NP_001091742                                                           15    9605
NP_001137450                                                           15    9605
NP_001788                                                              15    9605
NP_002121                                                              15    9605
XP_005255824                                                           15    9605
XP_005255823                                                           15    9605
XP_005255821                                                           15    9605
XP_005255820                                                           15    9605
XP_005255819                                                           15    9605
XP_005255818                                                           15    9605
XP_006718827                                                           15    9605
XP_005248352                                                           15    9605
XP_006711543                                                           15    9605
XP_005245461                                                           15    9605
NP_115536                                                              15    9605
NP_776159                                                              15    9605
>NP_008835
          Length = 4128

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SLYSLKIEV 9
            SLYS KIEV
Sbjct: 2544 SLYSPKIEV 2552
>NP_001075109
          Length = 4097

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SLYSLKIEV 9
            SLYS KIEV
Sbjct: 2544 SLYSPKIEV 2552
>NP_001269504
          Length = 91

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 8  IYSLKIE 14
>NP_001269507
          Length = 91

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 8  IYSLKIE 14
>NP_001269506
          Length = 91

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 8  IYSLKIE 14
>NP_954595
          Length = 170

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 87 IYSLKIE 93
>NP_954673
          Length = 370

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LYSLKIE 8
           +YSLKIE
Sbjct: 287 IYSLKIE 293
>NP_071887
          Length = 103

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 20 IYSLKIE 26
>NP_001027459
          Length = 147

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 64 IYSLKIE 70
>NP_068823
          Length = 170

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 87 IYSLKIE 93
>NP_001244328
          Length = 105

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 22 IYSLKIE 28
>NP_001244327
          Length = 105

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 22 IYSLKIE 28
>NP_001244324
          Length = 144

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 61 IYSLKIE 67
>NP_001244322
          Length = 170

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 87 IYSLKIE 93
>NP_001269508
          Length = 105

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 22 IYSLKIE 28
>NP_001269505
          Length = 105

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 22 IYSLKIE 28
>NP_001244326
          Length = 105

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 22 IYSLKIE 28
>NP_001244323
          Length = 103

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 20 IYSLKIE 26
>XP_006726463
          Length = 119

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 36 IYSLKIE 42
>XP_006712963
          Length = 119

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 36 IYSLKIE 42
>XP_006710204
          Length = 119

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLKIE
Sbjct: 36 IYSLKIE 42
>NP_003341
          Length = 145

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLK+E
Sbjct: 62 IYSLKVE 68
>XP_005251357
          Length = 105

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  LYSLKIE 8
          +YSLK+E
Sbjct: 22 IYSLKVE 28
>NP_001138775
          Length = 712

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LYSLKIEV 9
           LY LK+E+
Sbjct: 317 LYELKVEL 324
>NP_653287
          Length = 706

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LYSLKIEV 9
           LY LK+E+
Sbjct: 311 LYELKVEL 318
>NP_006718
          Length = 788

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LYSLKIE 8
           LY+LK+E
Sbjct: 343 LYTLKVE 349
>NP_001008781
          Length = 4557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   YSLKIE 8
           YSLKIE
Sbjct: 900 YSLKIE 905

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    SLYSLKIEV 9
            S+Y++ +EV
Sbjct: 1427 SIYNMSVEV 1435
>NP_001273384
          Length = 405

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  SLYSLKIEV 9
          +LY+L+IE+
Sbjct: 19 TLYALRIEL 27
>NP_068764
          Length = 405

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  SLYSLKIEV 9
          +LY+L+IE+
Sbjct: 19 TLYALRIEL 27
>NP_001273385
          Length = 361

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SLYSLKIEV 9
           +LY+L+IE+
Sbjct: 300 TLYALRIEL 308
>XP_006726250
          Length = 616

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LYSLKIEV 9
           LY LK+E+
Sbjct: 221 LYELKVEL 228
>XP_006726210
          Length = 616

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LYSLKIEV 9
           LY LK+E+
Sbjct: 221 LYELKVEL 228
>XP_006726112
          Length = 616

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LYSLKIEV 9
           LY LK+E+
Sbjct: 221 LYELKVEL 228
>XP_006726013
          Length = 616

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LYSLKIEV 9
           LY LK+E+
Sbjct: 221 LYELKVEL 228
>XP_006725925
          Length = 616

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LYSLKIEV 9
           LY LK+E+
Sbjct: 221 LYELKVEL 228
>XP_006725825
          Length = 616

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LYSLKIEV 9
           LY LK+E+
Sbjct: 221 LYELKVEL 228
>XP_006725698
          Length = 616

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LYSLKIEV 9
           LY LK+E+
Sbjct: 221 LYELKVEL 228
>XP_005249342
          Length = 694

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SLYSLKIEV 9
           +LY+L+IE+
Sbjct: 300 TLYALRIEL 308
>NP_056297
          Length = 686

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SLYSLKIEV 9
           +LY+L+IE+
Sbjct: 300 TLYALRIEL 308
>NP_443187
          Length = 710

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   YSLKIEV 9
           Y+LKIE+
Sbjct: 116 YTLKIEI 122
>NP_071410
          Length = 691

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SLYSLK 6
           SLYSLK
Sbjct: 666 SLYSLK 671
>NP_001186562
          Length = 691

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SLYSLK 6
           SLYSLK
Sbjct: 666 SLYSLK 671
>NP_001017403
          Length = 967

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LYSLKI 7
           LYSLKI
Sbjct: 113 LYSLKI 118
>NP_067649
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  LYSLKI 7
          LYSLKI
Sbjct: 61 LYSLKI 66
>NP_001017404
          Length = 828

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  LYSLKI 7
          LYSLKI
Sbjct: 70 LYSLKI 75
>NP_001091742
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SLYSLKI 7
           +LYSLK+
Sbjct: 382 TLYSLKV 388
>NP_001137450
          Length = 403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SLYSLKIEV 9
           SLY +++EV
Sbjct: 146 SLYVIRLEV 154
>NP_001788
          Length = 796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YSLKIE 8
           YSLK+E
Sbjct: 343 YSLKVE 348
>NP_002121
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SLYSLKI 7
           +LYSLK+
Sbjct: 382 TLYSLKV 388
>XP_005255824
          Length = 560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YSLKIE 8
           YSLK+E
Sbjct: 343 YSLKVE 348
>XP_005255823
          Length = 565

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YSLKIE 8
           YSLK+E
Sbjct: 343 YSLKVE 348
>XP_005255821
          Length = 693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YSLKIE 8
           YSLK+E
Sbjct: 343 YSLKVE 348
>XP_005255820
          Length = 796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YSLKIE 8
           YSLK+E
Sbjct: 343 YSLKVE 348
>XP_005255819
          Length = 796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YSLKIE 8
           YSLK+E
Sbjct: 343 YSLKVE 348
>XP_005255818
          Length = 796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YSLKIE 8
           YSLK+E
Sbjct: 343 YSLKVE 348
>XP_006718827
          Length = 3479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   SLYSLKIEV 9
           S+Y++ +EV
Sbjct: 317 SIYNMSVEV 325
>XP_005248352
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SLYSLKI 7
           +LYSLK+
Sbjct: 340 TLYSLKV 346
>XP_006711543
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LYSLKI 7
           LYSLKI
Sbjct: 113 LYSLKI 118
>XP_005245461
          Length = 924

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  LYSLKI 7
          LYSLKI
Sbjct: 70 LYSLKI 75
>NP_115536
          Length = 1222

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    SLYSLK 6
            SLYSLK
Sbjct: 1057 SLYSLK 1062
>NP_776159
          Length = 402

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SLYSLKIEV 9
           SLY +++EV
Sbjct: 146 SLYVIRLEV 154
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.133    0.316 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 456,022
Number of extensions: 766
Number of successful extensions: 62
Number of sequences better than 10000.0: 61
Number of HSP's gapped: 62
Number of HSP's successfully gapped: 62
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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