Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_057323                                                              17    4312
XP_005256732                                                           17    4312
NP_001139017                                                           17    4312
NP_001273479                                                           16    5631
NP_733751                                                              16    7355
XP_006716142                                                           16    7355
XP_005250088                                                           16    7355
XP_006716141                                                           16    7355
XP_005250085                                                           16    7355
XP_006716140                                                           16    7355
XP_005250084                                                           16    7355
XP_005250083                                                           16    7355
XP_005250082                                                           16    7355
XP_006715469                                                           16    7355
XP_005266931                                                           16    7355
NP_055928                                                              15    9605
NP_003382                                                              15    9605
NP_113621                                                              15    9605
NP_853514                                                              15    9605
NP_055616                                                              15    9605
XP_005256618                                                           15    9605
XP_005256617                                                           15    9605
XP_006716172                                                           15    9605
XP_006711879                                                           15    9605
XP_005273329                                                           15    9605
>NP_057323
          Length = 3530

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SLVSSHRQ 8
            +LVS HRQ
Sbjct: 3383 NLVSQHRQ 3390
>XP_005256732
          Length = 3531

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SLVSSHRQ 8
            +LVS HRQ
Sbjct: 3384 NLVSQHRQ 3391
>NP_001139017
          Length = 154

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLVSSHRQM 9
           SLV SHR++
Sbjct: 101 SLVGSHRRV 109
>NP_001273479
          Length = 153

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  SLVSSHRQ 8
          SLVS+H Q
Sbjct: 90 SLVSAHNQ 97
>NP_733751
          Length = 4911

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    LVSSHR 7
            LVSSHR
Sbjct: 4126 LVSSHR 4131
>XP_006716142
          Length = 4927

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    LVSSHR 7
            LVSSHR
Sbjct: 4142 LVSSHR 4147
>XP_005250088
          Length = 4928

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    LVSSHR 7
            LVSSHR
Sbjct: 4143 LVSSHR 4148
>XP_006716141
          Length = 4959

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    LVSSHR 7
            LVSSHR
Sbjct: 4174 LVSSHR 4179
>XP_005250085
          Length = 4979

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    LVSSHR 7
            LVSSHR
Sbjct: 4198 LVSSHR 4203
>XP_006716140
          Length = 4982

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    LVSSHR 7
            LVSSHR
Sbjct: 4197 LVSSHR 4202
>XP_005250084
          Length = 4982

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    LVSSHR 7
            LVSSHR
Sbjct: 4197 LVSSHR 4202
>XP_005250083
          Length = 4982

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    LVSSHR 7
            LVSSHR
Sbjct: 4197 LVSSHR 4202
>XP_005250082
          Length = 4983

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    LVSSHR 7
            LVSSHR
Sbjct: 4198 LVSSHR 4203
>XP_006715469
          Length = 1235

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SLVSSHRQ 8
          SL+SSH++
Sbjct: 71 SLISSHQR 78
>XP_005266931
          Length = 1283

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SLVSSHRQ 8
           SL+SSH++
Sbjct: 119 SLISSHQR 126
>NP_055928
          Length = 2961

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  SLVSSHR 7
          SLVS HR
Sbjct: 71 SLVSRHR 77
>NP_003382
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LVSSHRQM 9
          LVSS RQ+
Sbjct: 47 LVSSQRQL 54
>NP_113621
          Length = 579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LVSSHRQM 9
           LVSSH ++
Sbjct: 385 LVSSHHEL 392
>NP_853514
          Length = 1732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    SLVSSH 6
            SLVSSH
Sbjct: 1042 SLVSSH 1047
>NP_055616
          Length = 2137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    LVSSHRQM 9
            L+S+HR++
Sbjct: 1210 LISNHRRL 1217
>XP_005256618
          Length = 2962

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  SLVSSHR 7
          SLVS HR
Sbjct: 71 SLVSRHR 77
>XP_005256617
          Length = 2966

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  SLVSSHR 7
          SLVS HR
Sbjct: 71 SLVSRHR 77
>XP_006716172
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LVSSHRQM 9
          LVSS RQ+
Sbjct: 18 LVSSQRQL 25
>XP_006711879
          Length = 1364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    LVSSHRQM 9
            L+S+HR++
Sbjct: 1210 LISNHRRL 1217
>XP_005273329
          Length = 1934

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    LVSSHRQM 9
            L+S+HR++
Sbjct: 1007 LISNHRRL 1014
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.113    0.283 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 571,138
Number of extensions: 786
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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