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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_005249952                                                           17    3301
XP_005249951                                                           17    3301
NP_000522                                                              16    5631
NP_000512                                                              16    5631
NP_996880                                                              16    5631
NP_001104026                                                           16    7355
NP_689957                                                              16    7355
NP_001447                                                              16    7355
NP_849172                                                              16    7355
NP_054790                                                              16    7355
NP_001085                                                              16    7355
XP_006723818                                                           16    7355
XP_006723817                                                           16    7355
XP_006723816                                                           16    7355
XP_006723815                                                           16    7355
XP_006723814                                                           16    7355
XP_006723813                                                           16    7355
XP_005260405                                                           16    7355
NP_057629                                                              16    7355
NP_742057                                                              15    9605
NP_001273548                                                           15    9605
NP_064537                                                              15    9605
NP_835258                                                              15    9605
NP_055379                                                              15    9605
NP_001273563                                                           15    9605
NP_001273547                                                           15    9605
NP_001273546                                                           15    9605
NP_001273551                                                           15    9605
NP_001273549                                                           15    9605
XP_005263467                                                           15    9605
XP_006716499                                                           15    9605
XP_005251241                                                           15    9605

>XP_005249952
          Length = 406

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LVFPEISV 9
           LV PEISV
Sbjct: 345 LVIPEISV 352



>XP_005249951
          Length = 419

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LVFPEISV 9
           LV PEISV
Sbjct: 345 LVIPEISV 352



>NP_000522
          Length = 354

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SLVFPE 6
           SLVFPE
Sbjct: 321 SLVFPE 326



>NP_000512
          Length = 556

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SLVFPEIS 8
           S+ FPE+S
Sbjct: 248 SITFPELS 255



>NP_996880
          Length = 470

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LVFPEISV 9
           LVFPE +V
Sbjct: 167 LVFPEAAV 174



>NP_001104026
          Length = 2647

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    SLVFPEISV 9
            SL  PEIS+
Sbjct: 2163 SLKIPEISI 2171



>NP_689957
          Length = 2213

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    LVFPEI 7
            LVFPE+
Sbjct: 1384 LVFPEV 1389



>NP_001447
          Length = 2639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    SLVFPEISV 9
            SL  PEIS+
Sbjct: 2155 SLKIPEISI 2163



>NP_849172
          Length = 1065

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VFPEISV 9
           +FPE+S+
Sbjct: 257 LFPEVSI 263



>NP_054790
          Length = 2063

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SLVFPEIS 8
            SLV PE+S
Sbjct: 1558 SLVHPELS 1565



>NP_001085
          Length = 512

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  SLVFPEISV 9
          SLVFP +++
Sbjct: 83 SLVFPAVTL 91



>XP_006723818
          Length = 1975

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SLVFPEIS 8
            SLV PE+S
Sbjct: 1558 SLVHPELS 1565



>XP_006723817
          Length = 2020

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SLVFPEIS 8
            SLV PE+S
Sbjct: 1515 SLVHPELS 1522



>XP_006723816
          Length = 2034

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SLVFPEIS 8
            SLV PE+S
Sbjct: 1558 SLVHPELS 1565



>XP_006723815
          Length = 2051

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SLVFPEIS 8
            SLV PE+S
Sbjct: 1546 SLVHPELS 1553



>XP_006723814
          Length = 2052

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SLVFPEIS 8
            SLV PE+S
Sbjct: 1547 SLVHPELS 1554



>XP_006723813
          Length = 2062

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SLVFPEIS 8
            SLV PE+S
Sbjct: 1558 SLVHPELS 1565



>XP_005260405
          Length = 2063

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SLVFPEIS 8
            SLV PE+S
Sbjct: 1558 SLVHPELS 1565



>NP_057629
          Length = 327

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VFPEIS 8
           VFPE+S
Sbjct: 164 VFPEVS 169



>NP_742057
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2 LVFPEISV 9
         L+FP+++V
Sbjct: 2 LLFPQVAV 9



>NP_001273548
          Length = 142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  LVFPEI 7
          LVFPE+
Sbjct: 34 LVFPEL 39



>NP_064537
          Length = 242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LVFPEI 7
           LVFPE+
Sbjct: 134 LVFPEL 139



>NP_835258
          Length = 397

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SLVFPEISV 9
           SLV+P+ S+
Sbjct: 336 SLVYPDTSL 344



>NP_055379
          Length = 402

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SLVFPEISV 9
           SLV+P+ S+
Sbjct: 341 SLVYPDTSL 349



>NP_001273563
          Length = 242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LVFPEI 7
           LVFPE+
Sbjct: 134 LVFPEL 139



>NP_001273547
          Length = 142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  LVFPEI 7
          LVFPE+
Sbjct: 34 LVFPEL 39



>NP_001273546
          Length = 142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  LVFPEI 7
          LVFPE+
Sbjct: 34 LVFPEL 39



>NP_001273551
          Length = 136

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  LVFPEI 7
          LVFPE+
Sbjct: 28 LVFPEL 33



>NP_001273549
          Length = 142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  LVFPEI 7
          LVFPE+
Sbjct: 34 LVFPEL 39



>XP_005263467
          Length = 386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SLVFPEISV 9
           SLV+P+ S+
Sbjct: 325 SLVYPDTSL 333



>XP_006716499
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2 LVFPEISV 9
         L+FP+++V
Sbjct: 2 LLFPQVAV 9



>XP_005251241
          Length = 571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  LVFPEISV 9
          L+FP+++V
Sbjct: 14 LLFPQVAV 21



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.140    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 586,551
Number of extensions: 789
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)