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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              18    1135
XP_005262424                                                           18    1135
NP_060127                                                              17    3301
XP_005264437                                                           17    3301
NP_982261                                                              17    3301
NP_001002243                                                           17    3301
NP_067047                                                              16    5631
XP_006717281                                                           16    5631
XP_006717279                                                           16    5631
XP_006717278                                                           16    5631
XP_006717277                                                           16    5631
XP_006717276                                                           16    5631
XP_006717275                                                           16    5631
XP_006717274                                                           16    5631
XP_006717273                                                           16    5631
XP_006717272                                                           16    5631
NP_056099                                                              16    7355
NP_078836                                                              16    7355
XP_005269208                                                           16    7355
XP_006719664                                                           16    7355
XP_005269207                                                           16    7355
XP_006714237                                                           16    7355
XP_006710565                                                           16    7355
XP_006710564                                                           16    7355
XP_005270743                                                           16    7355
NP_115536                                                              16    7355
NP_001135786                                                           15    9605
NP_001129522                                                           15    9605
NP_612461                                                              15    9605
NP_005738                                                              15    9605
NP_001003811                                                           15    9605
NP_543152                                                              15    9605
NP_112566                                                              15    9605
NP_031378                                                              15    9605

>NP_722576
          Length = 3080

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    SLTSFKSA 8
            SLTSFKSA
Sbjct: 1770 SLTSFKSA 1777



>XP_005262424
          Length = 2875

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    SLTSFKSA 8
            SLTSFKSA
Sbjct: 1565 SLTSFKSA 1572



>NP_060127
          Length = 909

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  SLTSFKS 7
          SLTSFKS
Sbjct: 77 SLTSFKS 83



>XP_005264437
          Length = 937

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  SLTSFKS 7
          SLTSFKS
Sbjct: 77 SLTSFKS 83



>NP_982261
          Length = 936

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  SLTSFKS 7
          SLTSFKS
Sbjct: 77 SLTSFKS 83



>NP_001002243
          Length = 908

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  SLTSFKS 7
          SLTSFKS
Sbjct: 77 SLTSFKS 83



>NP_067047
          Length = 2506

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    TSFKSAF 9
            T FKSAF
Sbjct: 2229 TGFKSAF 2235



>XP_006717281
          Length = 1702

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    TSFKSAF 9
            T FKSAF
Sbjct: 1425 TGFKSAF 1431



>XP_006717279
          Length = 2506

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    TSFKSAF 9
            T FKSAF
Sbjct: 2229 TGFKSAF 2235



>XP_006717278
          Length = 2566

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    TSFKSAF 9
            T FKSAF
Sbjct: 2289 TGFKSAF 2295



>XP_006717277
          Length = 2567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    TSFKSAF 9
            T FKSAF
Sbjct: 2290 TGFKSAF 2296



>XP_006717276
          Length = 2567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    TSFKSAF 9
            T FKSAF
Sbjct: 2290 TGFKSAF 2296



>XP_006717275
          Length = 2567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    TSFKSAF 9
            T FKSAF
Sbjct: 2290 TGFKSAF 2296



>XP_006717274
          Length = 2567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    TSFKSAF 9
            T FKSAF
Sbjct: 2290 TGFKSAF 2296



>XP_006717273
          Length = 2567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    TSFKSAF 9
            T FKSAF
Sbjct: 2290 TGFKSAF 2296



>XP_006717272
          Length = 2567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    TSFKSAF 9
            T FKSAF
Sbjct: 2290 TGFKSAF 2296



>NP_056099
          Length = 3375

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    SLTSFKSAF 9
            SLT FK  F
Sbjct: 1695 SLTQFKEEF 1703



>NP_078836
          Length = 686

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SLTSFKSAF 9
           SLT FK+ F
Sbjct: 414 SLTRFKTLF 422



>XP_005269208
          Length = 455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SLTSFKSAF 9
           SLT FK+ F
Sbjct: 414 SLTRFKTLF 422



>XP_006719664
          Length = 578

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SLTSFKSAF 9
           SLT FK+ F
Sbjct: 306 SLTRFKTLF 314



>XP_005269207
          Length = 685

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SLTSFKSAF 9
           SLT FK+ F
Sbjct: 413 SLTRFKTLF 421



>XP_006714237
          Length = 241

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  SLTSFKSA 8
          SLTSF+ A
Sbjct: 4  SLTSFRQA 11



>XP_006710565
          Length = 3140

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    SLTSFKSAF 9
            SLT FK  F
Sbjct: 1460 SLTQFKEEF 1468



>XP_006710564
          Length = 3432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    SLTSFKSAF 9
            SLT FK  F
Sbjct: 1752 SLTQFKEEF 1760



>XP_005270743
          Length = 3453

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    SLTSFKSAF 9
            SLT FK  F
Sbjct: 1773 SLTQFKEEF 1781



>NP_115536
          Length = 1222

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SLTSFKSAF 9
           SLT F +AF
Sbjct: 183 SLTGFATAF 191



>NP_001135786
          Length = 166

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TSFKSAF 9
           TSF SAF
Sbjct: 120 TSFTSAF 126



>NP_001129522
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LTSFKSAF 9
           L  FKSAF
Sbjct: 216 LKQFKSAF 223



>NP_612461
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LTSFKSAF 9
           L  FKSAF
Sbjct: 216 LKQFKSAF 223



>NP_005738
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTSFKSAF 9
           +TSF SAF
Sbjct: 235 VTSFVSAF 242



>NP_001003811
          Length = 940

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LTSFKSAF 9
           LTSFK ++
Sbjct: 900 LTSFKESY 907



>NP_543152
          Length = 217

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TSFKSAF 9
           TSF SAF
Sbjct: 171 TSFTSAF 177



>NP_112566
          Length = 925

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LTSFKSAF 9
           LTSFK ++
Sbjct: 885 LTSFKESY 892



>NP_031378
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTSFKSAF 9
           +TSF SAF
Sbjct: 235 VTSFVSAF 242



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.120    0.300 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 322,482
Number of extensions: 321
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)